##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527659_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10544139 Sequences flagged as poor quality 0 Sequence length 52 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.879118247587595 33.0 31.0 34.0 30.0 34.0 2 32.29698660080259 34.0 31.0 34.0 30.0 34.0 3 32.424480557397814 34.0 31.0 34.0 30.0 34.0 4 35.94151129836206 37.0 35.0 37.0 35.0 37.0 5 36.06705573589271 37.0 35.0 37.0 35.0 37.0 6 36.2065931604278 37.0 35.0 37.0 35.0 37.0 7 36.36717810719301 37.0 36.0 37.0 35.0 37.0 8 36.48440237747245 37.0 37.0 37.0 35.0 37.0 9 38.481951252729125 39.0 39.0 39.0 37.0 39.0 10 37.675226493125706 39.0 38.0 39.0 35.0 39.0 11 37.2486896274793 39.0 37.0 39.0 34.0 39.0 12 36.21185266999989 38.0 35.0 39.0 32.0 39.0 13 35.8904880711455 39.0 35.0 39.0 31.0 39.0 14 36.79030919452029 40.0 35.0 40.0 31.0 40.0 15 37.15198566710853 40.0 36.0 40.0 32.0 40.0 16 37.10940921776543 40.0 35.0 40.0 32.0 40.0 17 37.169392114424895 40.0 35.0 40.0 32.0 40.0 18 37.17223729694762 40.0 35.0 40.0 32.0 40.0 19 36.99780437264721 40.0 35.0 40.0 32.0 40.0 20 37.11361525108878 40.0 35.0 40.0 32.0 40.0 21 37.10697829381802 40.0 35.0 40.0 32.0 40.0 22 37.14977695191613 40.0 35.0 40.0 32.0 40.0 23 37.13459012632516 40.0 35.0 40.0 32.0 40.0 24 36.775514624759786 39.0 35.0 40.0 32.0 40.0 25 36.29599420113866 37.0 35.0 40.0 31.0 40.0 26 35.62974008593779 37.0 35.0 40.0 30.0 40.0 27 35.36028223831268 36.0 35.0 40.0 30.0 40.0 28 35.2030215079676 36.0 35.0 40.0 29.0 40.0 29 35.09282095010318 36.0 35.0 40.0 28.0 40.0 30 34.758869074089404 36.0 35.0 40.0 25.0 40.0 31 34.51169346306986 35.0 34.0 40.0 24.0 40.0 32 34.07231951323859 35.0 34.0 40.0 22.0 40.0 33 33.823217808490575 35.0 33.0 40.0 21.0 40.0 34 33.49289629053638 35.0 33.0 40.0 19.0 40.0 35 33.1536897417608 35.0 33.0 40.0 17.0 40.0 36 32.7116126788541 35.0 33.0 40.0 15.0 40.0 37 32.053392505542654 35.0 31.0 40.0 11.0 40.0 38 31.443857103932338 35.0 30.0 40.0 10.0 40.0 39 30.703650909761336 35.0 25.0 40.0 9.0 40.0 40 29.97682722126482 35.0 23.0 40.0 8.0 40.0 41 29.35974203299103 35.0 20.0 39.0 8.0 40.0 42 28.916956614475588 35.0 18.0 39.0 8.0 40.0 43 28.4728657313793 35.0 17.0 39.0 8.0 40.0 44 28.171557488003526 35.0 15.0 38.0 8.0 40.0 45 28.023631422157845 35.0 15.0 38.0 8.0 40.0 46 27.815479291386428 34.0 15.0 38.0 8.0 40.0 47 27.53537344300943 34.0 13.0 37.0 8.0 40.0 48 27.30691202003312 34.0 12.0 37.0 8.0 40.0 49 27.1104464764738 34.0 12.0 37.0 8.0 40.0 50 26.719520579157766 33.0 10.0 36.0 8.0 40.0 51 26.402221746128347 33.0 10.0 36.0 8.0 40.0 52 25.815643268739155 33.0 10.0 35.0 8.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 13.0 11 24.0 12 47.0 13 96.0 14 245.0 15 1053.0 16 4157.0 17 9573.0 18 17531.0 19 29141.0 20 45466.0 21 67167.0 22 98873.0 23 147992.0 24 208668.0 25 276008.0 26 363952.0 27 471293.0 28 572590.0 29 594026.0 30 482972.0 31 378973.0 32 380799.0 33 435106.0 34 648316.0 35 726222.0 36 1075671.0 37 1283588.0 38 1755634.0 39 468942.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.21344929159223 0.5392095077654041 0.16665182429783976 0.08068937634452657 8 97.80169817564051 1.4904868002973026 0.4642768840585277 0.24353814000365512 9 95.39807849649934 2.643165079671275 1.1723384906060135 0.7864179332233765 10 64.01882600371638 23.61678843573667 4.8171690452866756 7.547216515260279 11 47.20787538935137 30.730418102416895 10.920351106904034 11.141355401327695 12 34.12605808781542 36.969438661610965 14.125923415842678 14.778579834730934 13 22.60399829706342 44.17119311496178 15.280413128089453 17.944395459885346 14 21.638523543743116 50.60005373601392 13.032244738048313 14.729177982194658 15 24.607063696713407 49.88246076801529 12.035710075521576 13.474765459749724 16 22.418795882717404 51.14724872272644 15.740450690189117 10.693504704367042 17 26.010924173135425 51.06774483909972 11.588115444987968 11.333215542776893 18 23.63954041197674 50.36899646334329 13.655880295204758 12.335582829475218 19 21.109035076263694 50.55285215796188 16.538780454240975 11.799332311533451 20 21.664594899593034 51.40278404903425 12.143229523055416 14.789391528317294 21 19.653762151656004 47.79203878097586 17.596202022754063 14.957997044614075 22 18.64501217216503 49.83455737827432 15.10278838319563 16.41764206636502 23 19.18702892668619 51.08526167949796 14.895346125463634 14.83236326835221 24 18.439542574315457 49.08967910988275 19.04335669323024 13.427421622571552 25 19.71016315319819 50.18482779864719 15.38245085729617 14.722558190858448 26 19.49465006104339 50.68627225039427 16.17197952341106 13.647098165151275 27 17.391329913234262 50.15391963250864 18.931076306941705 13.523674147315395 28 20.985696413903497 51.85259792193558 15.36787403883807 11.793831625322845 29 22.657155790529696 49.4713982810735 15.07505733754079 12.79638859085602 30 21.455692114832704 49.514626087535454 14.937075469130292 14.09260632850155 31 24.82711011302108 47.56351372075046 14.227259333360456 13.382116832868004 32 23.566466640851377 47.502626814764106 16.529220640964613 12.4016859034199 33 25.693629418200953 46.26955316123962 14.752223960628744 13.284593459930678 34 24.069713041529518 44.607169916860926 17.11827774652819 14.204839295081372 35 24.785257478111774 45.53958364926714 16.981367563534587 12.6937913090865 36 25.859019878247054 43.77383492383778 16.55793801656067 13.809207181354493 37 24.429989020440644 43.782579118124296 18.029855258926307 13.75757660250875 38 26.398447516672537 43.13403872995225 16.34499507261807 14.122518680757148 39 24.859346031003575 42.04163090035137 16.311810760461334 16.787212308183722 40 24.493341751280024 41.59550628078784 18.757539140938867 15.153612826993271 41 24.17525982918093 41.91302865032413 18.323791065349194 15.587920455145746 42 25.492949210931304 41.72438356512561 18.04449846497661 14.738168758966474 43 25.862576356400464 41.191300683725814 18.41844080393857 14.527682155935159 44 25.32103379896642 44.52250676892632 15.768475738037976 14.387983694069284 45 24.370486769948688 45.40613510500952 15.879143854230298 14.34423427081149 46 23.43221196154565 46.6289471335687 15.592207196813321 14.346633708072323 47 24.4325496847111 44.80719573215035 15.722108746859274 15.038145836279282 48 24.05750720850702 44.95796195402963 16.023138541705492 14.961392295757861 49 23.29688559682303 45.274754060051755 16.21005754950689 15.218302793618331 50 20.841739662195273 46.2661958458628 16.71746740060995 16.17459709133197 51 20.073028248205 45.67587737604749 17.625042689592767 16.626051686154746 52 21.53626768387632 43.60760039297661 18.117003199597427 16.739128723549644 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2922.0 1 2668.5 2 2415.0 3 4336.5 4 6258.0 5 42852.0 6 79446.0 7 93299.5 8 107153.0 9 175218.5 10 243284.0 11 190133.0 12 136982.0 13 113508.0 14 94793.5 15 99553.0 16 115624.0 17 131695.0 18 157843.0 19 183991.0 20 214143.0 21 244295.0 22 281479.0 23 318663.0 24 357358.5 25 396054.0 26 421401.5 27 446749.0 28 456082.0 29 465415.0 30 451980.5 31 438546.0 32 449435.5 33 460325.0 34 500050.0 35 539775.0 36 525092.5 37 510410.0 38 515342.0 39 544043.0 40 567812.0 41 608050.5 42 648289.0 43 672282.0 44 696275.0 45 652352.5 46 608430.0 47 593236.0 48 578042.0 49 540130.0 50 502218.0 51 428122.5 52 354027.0 53 317404.0 54 280781.0 55 247178.0 56 213575.0 57 203854.0 58 194133.0 59 169270.5 60 144408.0 61 128945.5 62 113483.0 63 92572.0 64 60058.5 65 48456.0 66 42180.5 67 35905.0 68 34522.5 69 33140.0 70 25604.5 71 18069.0 72 12328.0 73 6587.0 74 4620.5 75 2654.0 76 1842.0 77 1030.0 78 787.0 79 544.0 80 356.5 81 169.0 82 136.0 83 103.0 84 75.0 85 47.0 86 41.0 87 35.0 88 31.0 89 13.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1.0544139E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.020248939326066 #Duplication Level Percentage of deduplicated Percentage of total 1 79.27976595370038 24.59278075735293 2 9.792143275698253 6.075094441234151 3 3.878394328489729 3.6092627266386526 4 1.8517594146259482 2.2976815206975054 5 1.029041199399635 1.5960557087099672 6 0.6521233143285351 1.2137416529765723 7 0.46643330300947144 1.0128214021062139 8 0.3287144853269736 0.8157444131844135 9 0.2662444703673362 0.7433072774562411 >10 1.8572369452365463 11.880468224470343 >50 0.34031495392963906 7.406452206564082 >100 0.22431735799799965 12.926688568549485 >500 0.01853674354015558 3.933990090376241 >1k 0.01266115816900155 7.498490662815745 >5k 0.0011261126141645032 2.4764352655271593 >10k+ 0.0011869835662815032 11.920985081340548 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 161210 1.5289062482958542 No Hit AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 76733 0.727731301721269 Illumina Single End Adapter 2 (100% over 34bp) AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG 75575 0.7167488971835443 No Hit AATAAAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 72928 0.6916449033913532 Illumina Single End Adapter 2 (100% over 34bp) AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64638 0.6130230263466747 No Hit AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55920 0.5303420222362395 No Hit AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 55917 0.5303135704110122 Illumina Single End Adapter 2 (97% over 34bp) AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 52189 0.49495743559526295 Illumina Single End Adapter 2 (97% over 34bp) AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA 51559 0.48898255229753707 Illumina Single End Adapter 2 (100% over 34bp) AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50989 0.4835767055043565 No Hit AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA 43418 0.4117737825724794 No Hit AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33279 0.3156160972460625 No Hit AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA 31645 0.3001193364389449 No Hit AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA 30272 0.28709788442659945 No Hit AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 29414 0.2789606624116014 Illumina Single End Adapter 2 (97% over 34bp) AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA 29122 0.27619135142281415 Illumina Single End Adapter 2 (97% over 34bp) AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA 27839 0.2640234541672867 No Hit AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA 26057 0.24712306998229064 No Hit AATAAAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 24162 0.22915100038040087 Illumina Single End Adapter 2 (100% over 34bp) AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG 21253 0.20156221385169526 No Hit AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA 19124 0.18137090188207874 Illumina Single End Adapter 2 (100% over 34bp) AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG 16032 0.15204655401450987 No Hit AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA 15501 0.14701058094928376 Illumina Single End Adapter 2 (100% over 34bp) AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA 14531 0.13781115745913441 Illumina Single End Adapter 2 (97% over 34bp) AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA 14511 0.13762147862428598 No Hit AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13532 0.12833669965845482 No Hit AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA 13390 0.1269899799310309 No Hit AATAAAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA 12996 0.12325330688451659 Illumina Single End Adapter 2 (100% over 34bp) AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12950 0.1228170455643652 No Hit AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG 12746 0.12088232144891109 No Hit AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12692 0.1203701885948203 No Hit AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12556 0.1190803725178509 No Hit AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA 12075 0.11451859653974591 No Hit AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA 11786 0.11177773737618595 No Hit AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA 11522 0.10927397675618652 No Hit AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11115 0.10541401246702078 No Hit AATAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 10895 0.10332754528368793 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.4299924346596721 0.0 0.0 0.0 0.0 7 0.43610957708353426 0.0 0.0 0.0 0.0 8 0.8719725716817656 0.0 0.0 0.0 0.0 9 1.9711709035702203 0.0 0.0 0.0 0.0 10 3.038427319670198 0.0 0.0 0.0 0.0 11 3.985417870534522 0.0 0.0 0.0 0.0 12 4.465485517594182 0.0 0.0 0.0 0.0 13 4.8853775542981746 0.0 0.0 0.0 0.0 14 5.2449896572873325 0.0 0.0 0.0 9.483941742422023E-6 15 5.567851486024606 0.0 0.0 0.0 9.483941742422023E-6 16 5.838011050499239 0.0 0.0 0.0 1.8967883484844046E-5 17 6.095528520631224 0.0 0.0 0.0 1.8967883484844046E-5 18 6.327799737844883 0.0 0.0 0.0 1.8967883484844046E-5 19 6.559653661621874 0.0 0.0 0.0 1.8967883484844046E-5 20 6.788415820390835 0.0 0.0 0.0 1.8967883484844046E-5 21 7.028549225308961 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 22 7.290543115943369 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 23 7.601303435017312 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 24 7.902153034970423 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 25 8.214127298587396 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 26 8.53084353307558 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 27 8.833115724290053 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 28 9.116306224718775 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 29 9.330377757728725 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 30 9.56247826399102 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 31 9.718318394702498 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 32 9.880114440828217 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 33 10.03443714086091 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 34 10.186113821147464 9.483941742422023E-6 0.0 0.0 1.8967883484844046E-5 35 10.362448749964317 9.483941742422023E-6 0.0 0.0 2.8451825227266068E-5 36 10.502204115480648 9.483941742422023E-6 0.0 0.0 2.8451825227266068E-5 37 10.641276637191524 1.8967883484844046E-5 0.0 0.0 3.793576696968809E-5 38 10.776271063953159 1.8967883484844046E-5 0.0 0.0 3.793576696968809E-5 39 10.908828117686992 1.8967883484844046E-5 0.0 0.0 3.793576696968809E-5 40 11.046553919670444 1.8967883484844046E-5 0.0 0.0 3.793576696968809E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTAA 35 1.02063495E-7 46.000004 41 AGGGATT 6275 0.0 41.528286 6 AAGCAGT 33260 0.0 40.65454 10 GGAAGCA 40185 0.0 40.316536 8 GAAGCAG 36565 0.0 40.269653 9 TACGCGT 80 0.0 40.25 43 AAGGGAT 29175 0.0 39.44096 5 GGGAAGC 44225 0.0 38.88547 7 AAGGGAC 39035 0.0 38.59357 5 AGGGATA 3185 0.0 37.912086 6 AGGGGTG 41780 0.0 37.86357 6 AGGGCGC 22400 0.0 37.785713 6 AAGGGGT 85585 0.0 37.40036 5 AGGGACG 22555 0.0 37.311905 6 AGGGATC 14710 0.0 36.93134 6 CGATCTA 100 0.0 36.800003 15 AGGGAAC 45555 0.0 36.54857 6 AGGGGCT 18505 0.0 36.541477 6 AGGGACT 4050 0.0 36.45926 6 CGATACG 95 0.0 36.315792 36 >>END_MODULE