FastQCFastQC Report
Fri 17 Jun 2016
SRR1527658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10605088
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1516421.4298985543542873No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA730310.6886411503610343Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA693960.6543651500110136Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG684180.6451431614711731No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA611140.5762705599425483No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA532320.5019477443280056Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524350.4944324837285651No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA494840.46660621769475175Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA483630.45603581978763397Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469930.44311749228294944No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA414520.3908689866599881No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA308290.2907000865999415No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA298560.281525245240775No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA286650.27029478680422075No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA282890.2667493188175336Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA275380.2596678122802941Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA265280.2501440817841398No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA249640.2353964436693029No Hit
AATAAAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA230590.217433367832497Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG203430.19182301929036327No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA178580.16839087049536977Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG158550.14950370991735287No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA150540.14195073157337307Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA136610.128815527037588Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA136120.1283534846669825No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA130470.12302585325081697No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127790.1204987643666889No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124320.11722675002791114No Hit
AATAAAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA121720.1147750966328615Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG121460.11452993129335656No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118030.11129563469911802No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117630.11091845725372576No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA116310.10967377168393133No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA112220.10581713230479559No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA111420.10506277741401109No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106530.10045178314409084No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT253.4190725E-546.039
AGGGATT60550.040.4921576
AAGCAGT328350.040.361210
TACGTAC402.9136027E-740.2538
GGAAGCA396200.040.212278
GAAGCAG360800.040.033269
AAGGGAT284550.039.590235
AAGGGAC403750.038.759635
GGGAAGC437450.038.723287
TACGCGT601.2369128E-1038.33333243
AGGGGTG418450.038.2609636
AGGGACG226350.038.155516
AGGGCGC222550.037.628856
AAGGGGT861650.037.565025
AGGGACT41700.037.285376
AGGGTAT88150.037.154856
AGGGGTC80750.037.084836
AGGGATC141500.036.946296
AAGGGTG176700.036.888515
AGGGATG63850.036.6703226
Produced by FastQC (version 0.11.5)