##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527658_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10605088
Sequences flagged as poor quality	0
Sequence length	52
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.845759412840327	33.0	31.0	34.0	30.0	34.0
2	32.27832960933469	34.0	31.0	34.0	30.0	34.0
3	32.40340165022676	34.0	31.0	34.0	30.0	34.0
4	35.91351047723508	37.0	35.0	37.0	35.0	37.0
5	36.07132350056878	37.0	35.0	37.0	35.0	37.0
6	36.14323539795238	37.0	35.0	37.0	35.0	37.0
7	36.32895578047066	37.0	35.0	37.0	35.0	37.0
8	36.458198555259514	37.0	37.0	37.0	35.0	37.0
9	38.43558459863794	39.0	39.0	39.0	37.0	39.0
10	37.58589725988129	39.0	37.0	39.0	35.0	39.0
11	37.214200297064956	39.0	37.0	39.0	34.0	39.0
12	36.17385984915919	38.0	35.0	39.0	31.0	39.0
13	35.70722854916433	38.0	35.0	39.0	30.0	39.0
14	36.73657295441584	39.0	35.0	40.0	31.0	40.0
15	37.10922125304382	40.0	36.0	40.0	32.0	40.0
16	37.10554575313284	40.0	35.0	40.0	32.0	40.0
17	37.194877968009315	40.0	35.0	40.0	32.0	40.0
18	37.2107364879952	40.0	35.0	40.0	32.0	40.0
19	36.993398734645105	40.0	35.0	40.0	32.0	40.0
20	37.11028121595974	40.0	35.0	40.0	32.0	40.0
21	37.15778803532795	40.0	35.0	40.0	32.0	40.0
22	37.088992472292546	40.0	35.0	40.0	32.0	40.0
23	37.1038100768235	40.0	35.0	40.0	32.0	40.0
24	36.802674621841895	39.0	35.0	40.0	32.0	40.0
25	36.29774246097722	37.0	35.0	40.0	31.0	40.0
26	35.5897964260174	37.0	35.0	40.0	30.0	40.0
27	35.322054941929764	36.0	35.0	40.0	30.0	40.0
28	35.18418300725086	36.0	35.0	40.0	29.0	40.0
29	35.0768056804432	36.0	35.0	40.0	28.0	40.0
30	34.73477004622687	36.0	35.0	40.0	25.0	40.0
31	34.50776485777393	35.0	34.0	40.0	24.0	40.0
32	34.07117706142561	35.0	34.0	40.0	22.0	40.0
33	33.640994020983136	35.0	33.0	40.0	20.0	40.0
34	33.442404626911156	35.0	33.0	40.0	19.0	40.0
35	33.19351739466943	35.0	33.0	40.0	18.0	40.0
36	32.632886308911345	35.0	32.0	40.0	15.0	40.0
37	32.04660781692712	35.0	31.0	40.0	11.0	40.0
38	31.20664533853939	35.0	29.0	40.0	10.0	40.0
39	30.620047377258917	35.0	25.0	40.0	9.0	40.0
40	29.96147254977988	35.0	23.0	39.0	8.0	40.0
41	29.195530107812402	35.0	19.0	39.0	8.0	40.0
42	28.862215193310984	35.0	18.0	39.0	8.0	40.0
43	28.51475206994982	35.0	17.0	39.0	8.0	40.0
44	28.21041645293278	35.0	15.0	38.0	8.0	40.0
45	28.05751220546213	35.0	15.0	38.0	8.0	40.0
46	27.721330082315205	34.0	15.0	38.0	8.0	40.0
47	27.38784128901146	34.0	13.0	37.0	8.0	40.0
48	27.266505002127282	34.0	13.0	37.0	8.0	40.0
49	27.06579455069114	33.0	12.0	36.0	8.0	40.0
50	26.64008153444837	33.0	10.0	36.0	8.0	40.0
51	26.249782180025285	33.0	10.0	35.0	8.0	40.0
52	25.766485294605758	33.0	10.0	35.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	26.0
12	52.0
13	94.0
14	251.0
15	1076.0
16	4265.0
17	9661.0
18	17700.0
19	29317.0
20	45198.0
21	67571.0
22	99064.0
23	148937.0
24	211877.0
25	279789.0
26	370433.0
27	473876.0
28	577513.0
29	595786.0
30	481983.0
31	382855.0
32	389918.0
33	453779.0
34	662796.0
35	745416.0
36	1101496.0
37	1340654.0
38	1710145.0
39	403551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.19976147298354	0.5449365436665872	0.17142714893077737	0.08387483441910147
8	97.80587393522808	1.481449281703273	0.4668325241619872	0.2458442589066682
9	95.38292374377279	2.638742837400312	1.1898156809259857	0.7885177379009018
10	63.86439226152579	23.610996910162367	4.93412218738779	7.590488640924055
11	47.06855803553916	30.76650566218781	10.959965631591176	11.204970670681845
12	34.14925929893274	36.9135833667764	14.12807701359951	14.80908032069135
13	22.857217214982093	43.82174857954974	15.339052349212	17.981981856256166
14	21.702969367156594	50.399930674785544	13.060080218099085	14.837019739958782
15	24.667065468952263	49.6916762972641	12.06539728854678	13.575860945236853
16	22.446452118077662	50.98198147907872	15.769223225681861	10.802343177161756
17	26.076587011819228	50.88618783738522	11.6240053830765	11.413219767719042
18	23.70706400550377	50.20459990525302	13.707345002700592	12.38099108654261
19	21.18052202867152	50.39491421476182	16.54276701900069	11.881796737565969
20	21.73640614769062	51.20929689598049	12.220539801272748	14.83375715505614
21	19.738619802117626	47.59758712044634	17.679212091403674	14.984580986032364
22	18.92975334103781	49.528518763823556	15.09219914063891	16.449528754499728
23	19.287383565322607	50.93614499002743	14.913398172650712	14.863073271999253
24	18.541458590442623	48.90710949310369	19.094070695122944	13.457361221330743
25	19.86299406473572	49.9385577941456	15.447858612771531	14.750589528347149
26	19.700713468855703	50.38267480665885	16.23429244528664	13.682319279198815
27	17.532131746572965	49.93635130609006	18.915694051760816	13.61582289557616
28	21.062720083039384	51.619967698523574	15.460390333394688	11.85692188504235
29	22.72500709093597	49.298977999993966	15.169558234688859	12.806456674381202
30	21.504743760730697	49.40752023934172	14.98745696405348	14.1002790358741
31	24.8461493200245	47.43810706709836	14.325812289346395	13.389931323530742
32	23.608441532969834	47.38194534547945	16.6034265816559	12.406186539894813
33	25.642672649203853	46.09792016813062	14.927994939787393	13.331412242878136
34	24.09940398420079	44.56112952575217	17.136029422858158	14.203437067188881
35	24.85546560292569	45.452522411883805	17.005243143668398	12.686768841522106
36	26.149061657951357	43.51218962068019	16.582521521745033	13.756227199623424
37	24.580031773427997	43.68019388429403	18.003631841621683	13.736142500656289
38	26.571792709310856	42.783859973627756	16.2724533733242	14.371893943737197
39	24.965224239534834	41.96346131215507	16.335555159938327	16.73575928837177
40	24.562898487971058	41.559975739946715	18.73426227109101	15.142863500991222
41	24.50285183866461	41.650715203871954	18.32059290785706	15.525840049606376
42	25.466200751940953	41.73714541548358	18.057417345334617	14.739236487240841
43	25.88333071823638	41.199808997341655	18.395905814265756	14.52095447015621
44	25.365220920373314	44.52176162988935	15.758926281422653	14.354091168314682
45	24.3843521147585	45.40510177756186	15.888411298425812	14.322134809253823
46	23.624877040152803	46.438841431584535	15.636956525018933	14.299325003243727
47	24.6833312462848	44.63272723432375	15.759388323793258	14.92455319559819
48	24.083854844014496	44.93793922313516	16.02841013671928	14.949795796131065
49	23.632618607219477	45.17396743902549	16.06521322595343	15.1282007278016
50	21.06404020409826	46.2418510812923	16.571734246806816	16.122374467802626
51	20.10195483526398	45.661620158173136	17.610707237884306	16.62571776867858
52	21.58760021604724	43.629510665069446	18.065790684622325	16.717098434260986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2789.0
1	2519.5
2	2250.0
3	4250.5
4	6251.0
5	41328.5
6	76406.0
7	90947.0
8	105488.0
9	170874.0
10	236260.0
11	187954.5
12	139649.0
13	115698.0
14	95321.0
15	98895.0
16	114651.0
17	130407.0
18	155725.0
19	181043.0
20	210312.5
21	239582.0
22	278035.5
23	316489.0
24	354232.5
25	391976.0
26	419322.0
27	446668.0
28	456830.0
29	466992.0
30	455495.5
31	443999.0
32	454424.0
33	464849.0
34	504570.5
35	544292.0
36	532077.5
37	519863.0
38	523973.5
39	551107.0
40	574130.0
41	613928.5
42	653727.0
43	674830.0
44	695933.0
45	656415.5
46	616898.0
47	602102.0
48	587306.0
49	547962.0
50	508618.0
51	434861.0
52	361104.0
53	321529.0
54	281954.0
55	249078.5
56	216203.0
57	205079.5
58	193956.0
59	169953.5
60	145951.0
61	130069.5
62	114188.0
63	93786.5
64	61142.0
65	48899.0
66	42550.5
67	36202.0
68	34690.0
69	33178.0
70	25710.0
71	18242.0
72	12428.0
73	6614.0
74	4631.5
75	2649.0
76	1851.0
77	1053.0
78	797.5
79	542.0
80	364.5
81	187.0
82	138.0
83	89.0
84	73.0
85	57.0
86	40.0
87	23.0
88	22.0
89	10.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.0605088E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.21585555028576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50319406352621	24.505443661230846
2	10.298799127770295	6.42971651827773
3	3.9833423879122023	3.73030321765193
4	1.8766037905790762	2.343191714073406
5	1.023723277269363	1.597819897335279
6	0.6424689523442481	1.2033130807152883
7	0.47206968220345835	1.0315241306532723
8	0.3708344138146591	0.9260730795770635
9	0.27068588283024325	0.7604722276137404
>10	1.9621532520987048	12.491135812847267
>50	0.3470603400002356	7.5960529618838954
>100	0.216473678296358	12.531140286362735
>500	0.01797039513138723	3.8208383281489606
>1k	0.012409213287347658	7.371051868675071
>5k	0.0010443397199123804	2.3194198829130324
>10k+	0.0011672032163726606	11.342503332040389
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151642	1.4298985543542873	No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	73031	0.6886411503610343	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	69396	0.6543651500110136	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG	68418	0.6451431614711731	No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61114	0.5762705599425483	No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	53232	0.5019477443280056	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52435	0.4944324837285651	No Hit
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	49484	0.46660621769475175	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	48363	0.45603581978763397	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46993	0.44311749228294944	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA	41452	0.3908689866599881	No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30829	0.2907000865999415	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA	29856	0.281525245240775	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA	28665	0.27029478680422075	No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	28289	0.2667493188175336	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	27538	0.2596678122802941	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA	26528	0.2501440817841398	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA	24964	0.2353964436693029	No Hit
AATAAAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	23059	0.217433367832497	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG	20343	0.19182301929036327	No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	17858	0.16839087049536977	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG	15855	0.14950370991735287	No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	15054	0.14195073157337307	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	13661	0.128815527037588	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA	13612	0.1283534846669825	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA	13047	0.12302585325081697	No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12779	0.1204987643666889	No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12432	0.11722675002791114	No Hit
AATAAAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	12172	0.1147750966328615	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG	12146	0.11452993129335656	No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11803	0.11129563469911802	No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11763	0.11091845725372576	No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	11631	0.10967377168393133	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA	11222	0.10581713230479559	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA	11142	0.10506277741401109	No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10653	0.10045178314409084	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.4207791580795935	0.0	0.0	0.0	0.0
7	0.42649339637728606	0.0	0.0	0.0	0.0
8	0.8555610288193742	0.0	0.0	0.0	0.0
9	1.9330344076352786	0.0	0.0	0.0	0.0
10	2.985368909715789	0.0	0.0	0.0	0.0
11	3.917072635323724	0.0	0.0	0.0	0.0
12	4.387516633525342	0.0	0.0	0.0	9.429436134806237E-6
13	4.796942750498628	0.0	0.0	0.0	9.429436134806237E-6
14	5.153639460606079	0.0	0.0	0.0	9.429436134806237E-6
15	5.472080948314621	0.0	0.0	0.0	9.429436134806237E-6
16	5.738811408259885	0.0	0.0	0.0	1.8858872269612474E-5
17	5.99622558530396	0.0	0.0	0.0	1.8858872269612474E-5
18	6.228010554933632	0.0	0.0	0.0	1.8858872269612474E-5
19	6.455175100857249	0.0	0.0	0.0	1.8858872269612474E-5
20	6.681330697114442	0.0	0.0	0.0	1.8858872269612474E-5
21	6.917594648908147	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
22	7.173660416584945	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
23	7.4831816577099595	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
24	7.775126429879696	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
25	8.079301180716275	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
26	8.388247226237066	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
27	8.684416385795195	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
28	8.961010035937466	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
29	9.174454752284941	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
30	9.402439659152286	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
31	9.554366734156284	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
32	9.72022108633139	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
33	9.872949663406848	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
34	10.01935108883585	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
35	10.189439257835485	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
36	10.328891188833135	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
37	10.463062635595291	1.8858872269612474E-5	0.0	0.0	2.828830840441871E-5
38	10.59623456212716	1.8858872269612474E-5	0.0	0.0	5.657661680883742E-5
39	10.728274956322851	1.8858872269612474E-5	0.0	0.0	5.657661680883742E-5
40	10.863002739816963	1.8858872269612474E-5	0.0	0.0	5.657661680883742E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	25	3.4190725E-5	46.0	39
AGGGATT	6055	0.0	40.492157	6
AAGCAGT	32835	0.0	40.3612	10
TACGTAC	40	2.9136027E-7	40.25	38
GGAAGCA	39620	0.0	40.21227	8
GAAGCAG	36080	0.0	40.03326	9
AAGGGAT	28455	0.0	39.59023	5
AAGGGAC	40375	0.0	38.75963	5
GGGAAGC	43745	0.0	38.72328	7
TACGCGT	60	1.2369128E-10	38.333332	43
AGGGGTG	41845	0.0	38.260963	6
AGGGACG	22635	0.0	38.15551	6
AGGGCGC	22255	0.0	37.62885	6
AAGGGGT	86165	0.0	37.56502	5
AGGGACT	4170	0.0	37.28537	6
AGGGTAT	8815	0.0	37.15485	6
AGGGGTC	8075	0.0	37.08483	6
AGGGATC	14150	0.0	36.94629	6
AAGGGTG	17670	0.0	36.88851	5
AGGGATG	6385	0.0	36.670322	6
>>END_MODULE