FastQCFastQC Report
Fri 17 Jun 2016
SRR1527657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794328
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC72450.9120917303683113No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC42920.5403309464100473No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG29880.37616702420158926No Hit
ATTCCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA25380.3195153639302656No Hit
ATTCCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA20130.25342176028038793No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT18020.2268584262420562No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT15110.19022368593326686No Hit
ATTCCAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA14260.17952281677090573No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT12490.1572398303975184No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC12190.15346305304609684No Hit
ATTCCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG11200.14099968778640562No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11000.13848183621879123No Hit
ATTCCAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA10070.12677382642938434No Hit
ATTCCAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG10000.1258925783807193No Hit
ATTCCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA9410.11846491625625685No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG9250.11645063500216535No Hit
ATTCCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA9200.11582117211026176No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG9100.11456224632645456No Hit
ATTCCAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT9050.11393278343455096No Hit
ATTCCAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC8770.11040779123989083No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC8580.10801583225065717No Hit
ATTCCAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC7980.10046227754781399No Hit
ATTCCAGGGGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATC7960.10021049239105255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA302.5267254E-644.012
CTTAAGG254.4415938E-544.020
AACCGTT351.4453326E-744.026
CTATGCG207.855011E-444.031
ACACGTT254.4415938E-544.020
ACTGCGT351.4453326E-744.033
ACGCCGT207.855011E-444.018
GCGACGT302.5267254E-644.043
CGACGTT302.5267254E-644.044
CGAAAAT207.855011E-444.027
TAGACGG207.855011E-444.030
TAGCGTT207.855011E-444.042
ACCGATA207.855011E-444.010
ATAGCGT454.802132E-1044.025
ATTCGTT351.4453326E-744.014
TAACGTC207.855011E-444.022
TAACGGC254.4415938E-544.013
ATCGTTT254.4415938E-544.017
AACGGTA254.4415938E-544.044
TACATAC207.855011E-444.028
Produced by FastQC (version 0.11.5)