##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794328
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.838868830004735	33.0	31.0	34.0	3.0	34.0
2	30.345544913436264	34.0	31.0	34.0	16.0	34.0
3	32.41712365672619	34.0	31.0	34.0	28.0	34.0
4	36.10612366679759	37.0	35.0	37.0	35.0	37.0
5	36.37575787332185	37.0	37.0	37.0	35.0	37.0
6	36.34309252600941	37.0	37.0	37.0	35.0	37.0
7	36.54951355107713	37.0	37.0	37.0	35.0	37.0
8	36.67813673948294	37.0	37.0	37.0	35.0	37.0
9	38.74116863562659	39.0	39.0	39.0	39.0	39.0
10	38.31782336767683	39.0	39.0	39.0	37.0	39.0
11	38.12418421609209	39.0	39.0	39.0	37.0	39.0
12	37.73848712370708	39.0	37.0	39.0	35.0	39.0
13	37.62420939460777	39.0	37.0	39.0	35.0	39.0
14	38.345835725292325	40.0	38.0	40.0	35.0	40.0
15	38.452699640450795	40.0	38.0	40.0	35.0	40.0
16	38.49711076532616	40.0	38.0	40.0	35.0	40.0
17	38.40784537369953	40.0	38.0	40.0	35.0	40.0
18	38.379714677060356	40.0	38.0	40.0	35.0	40.0
19	38.38405545316292	40.0	38.0	40.0	35.0	40.0
20	38.30518878851054	40.0	38.0	40.0	34.0	40.0
21	38.30507422626421	40.0	38.0	40.0	35.0	40.0
22	38.29949970289351	40.0	38.0	40.0	35.0	40.0
23	38.224491645768495	40.0	38.0	40.0	35.0	40.0
24	38.18958666948666	40.0	38.0	40.0	35.0	40.0
25	38.12635586306916	40.0	38.0	40.0	35.0	40.0
26	38.03056671803084	40.0	38.0	40.0	34.0	40.0
27	37.930581573355084	40.0	37.0	40.0	34.0	40.0
28	37.81709570857379	40.0	37.0	40.0	34.0	40.0
29	37.77610130827568	40.0	37.0	40.0	34.0	40.0
30	37.905969574281656	40.0	38.0	40.0	34.0	40.0
31	37.93671883655115	40.0	37.0	40.0	34.0	40.0
32	37.94221404759747	40.0	37.0	40.0	34.0	40.0
33	37.86789084609884	40.0	37.0	40.0	34.0	40.0
34	37.549477042229405	40.0	37.0	40.0	33.0	40.0
35	37.54365702833086	40.0	36.0	40.0	33.0	40.0
36	37.54567886313966	40.0	36.0	40.0	33.0	40.0
37	37.451136054627305	40.0	36.0	40.0	33.0	40.0
38	37.35164692671038	40.0	36.0	40.0	33.0	40.0
39	37.22948706327865	40.0	35.0	40.0	33.0	40.0
40	37.04566627388182	40.0	35.0	40.0	33.0	40.0
41	36.88906345993091	40.0	35.0	40.0	33.0	40.0
42	36.85853702752515	40.0	35.0	40.0	32.0	40.0
43	36.769495724688035	40.0	35.0	40.0	32.0	40.0
44	36.401904502925746	39.0	35.0	40.0	31.0	40.0
45	36.37552748990341	39.0	35.0	40.0	31.0	40.0
46	36.222979675902145	39.0	35.0	40.0	31.0	40.0
47	36.16930033940639	39.0	35.0	40.0	31.0	40.0
48	36.09159062754932	39.0	35.0	40.0	31.0	40.0
49	36.02323725211751	38.0	35.0	40.0	31.0	40.0
50	35.5355533230605	37.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	4.0
15	5.0
16	20.0
17	54.0
18	88.0
19	142.0
20	261.0
21	473.0
22	784.0
23	1238.0
24	1763.0
25	2327.0
26	3223.0
27	4118.0
28	5710.0
29	7422.0
30	10034.0
31	13529.0
32	17045.0
33	24305.0
34	41618.0
35	56663.0
36	63214.0
37	134830.0
38	227284.0
39	178172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.25320522504558	0.5662648175564754	0.07515786929328942	0.10537208810466206
8	98.7163992708302	1.0102879415052723	0.21036649847418196	0.06294628919035965
9	97.61836420219355	1.5875054133808704	0.36773222145008105	0.42639816297549626
10	58.02162330926267	26.297323020213316	6.665886132680707	9.01516753784331
11	33.175715825200676	23.94464251543443	21.938292493780907	20.941349165583993
12	29.834400902398002	17.029992647873424	28.891717275483174	24.2438891742454
13	23.28131451994642	19.29114924816952	28.39368623540905	29.03384999647501
14	23.163101388846925	19.41477576013939	33.4008117553454	24.02131109566829
15	24.82324681995347	20.497703719370335	30.813089806729714	23.86595965394648
16	27.9937255138935	19.527575510368514	26.68973018702601	25.78896878871197
17	27.505262309776313	19.75833660654037	24.937934958858303	27.798466124825012
18	27.952684533341394	22.266872123354585	24.008847730408597	25.771595612895425
19	29.753829652234344	22.210346355661642	22.226712390851134	25.809111601252884
20	29.500659677110715	23.12709611143004	22.75495764973663	24.61728656172261
21	28.954789457251916	21.119361271414327	25.996691543040157	23.9291577282936
22	27.511556938695346	20.371937033567995	26.347805944143982	25.768700083592673
23	28.203084871740643	22.282356910495412	24.947251009658476	24.56730720810547
24	28.769097904140356	21.507991660875607	23.693738606721656	26.02917182826238
25	26.73568097813498	23.23536372883746	23.80125086865879	26.227704424368774
26	27.0822632464171	21.997713790776608	23.605487909276775	27.314535053529525
27	25.041922228600775	21.64106011622403	24.239860611737214	29.077157043437975
28	25.834290116929026	23.71224481574362	22.560831293873562	27.892633773453788
29	28.471613741426715	22.3575147797887	22.48315557301266	26.687715905771924
30	27.80199111701967	21.293093029579722	24.94951707606933	25.95539877733128
31	28.783323765497375	22.05801633582097	23.57363708694645	25.585022811735204
32	32.17361090129015	21.760783958264092	22.588527661117322	23.47707747932844
33	31.514815038623844	20.898671581512925	23.10078456254847	24.48572881731476
34	27.06690435185465	23.398772295575633	26.117296633128884	23.417026719440837
35	27.640596831535586	25.339909961627942	25.0489722129901	21.97052099384637
36	28.35868809861921	25.594212969957	22.481393076915328	23.565705854508465
37	29.16591131119638	25.327572488946632	21.703376942522485	23.8031392573345
38	28.853320039077058	25.092153367374685	22.739472862595804	23.315053730952453
39	28.054783414408153	23.332301014190612	21.942824626602615	26.670090944798623
40	28.18155724083754	23.471664098458067	22.74941837628788	25.597360284416514
41	25.179396924192528	25.738989435094823	23.284335941827557	25.797277698885097
42	25.33059391082777	23.659747610558863	23.2076673615937	27.80199111701967
43	26.519271635898523	22.19108479116939	23.811196382350868	27.478447190581225
44	26.221283902871356	21.473622986977674	23.893530128611857	28.411562981539113
45	27.181844275916244	22.446898510439013	22.863854730035953	27.507402483608782
46	26.093125258079787	25.026941011773474	22.394904875567775	26.485028854578964
47	25.291818996686505	24.027731617165703	24.928870693214893	25.751578692932892
48	26.897578833932585	22.525707264505343	24.948635828020667	25.62807807354141
49	27.650542345227663	24.35895499088538	23.005609773292647	24.984892890594313
50	24.843137847337623	24.16835362721697	24.39042813548056	26.59808038996485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	109.5
4	219.0
5	166.5
6	114.0
7	95.5
8	77.0
9	184.0
10	291.0
11	766.0
12	1241.0
13	1510.0
14	1779.0
15	1672.0
16	1565.0
17	1653.0
18	1741.0
19	1864.5
20	1988.0
21	1848.5
22	1709.0
23	2463.0
24	3217.0
25	3645.0
26	4073.0
27	4598.5
28	5124.0
29	6069.5
30	7015.0
31	7620.5
32	8226.0
33	11435.5
34	14645.0
35	13362.5
36	12080.0
37	15295.5
38	18511.0
39	17742.5
40	16974.0
41	21339.5
42	25705.0
43	26619.5
44	27534.0
45	29555.0
46	31576.0
47	35347.0
48	39118.0
49	43109.0
50	47100.0
51	47696.5
52	48293.0
53	53597.0
54	58901.0
55	60702.0
56	62503.0
57	67286.0
58	72069.0
59	72869.5
60	73670.0
61	69396.0
62	65122.0
63	54821.5
64	44521.0
65	38205.0
66	31889.0
67	26230.0
68	20571.0
69	19511.0
70	18451.0
71	15130.0
72	11809.0
73	9919.0
74	8029.0
75	5776.0
76	3523.0
77	2565.0
78	1607.0
79	1392.5
80	1178.0
81	798.5
82	419.0
83	256.5
84	94.0
85	71.5
86	49.0
87	28.5
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	794328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.524906587706841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4510897579931	6.615151423593277
2	9.343237420531596	1.7798692731466095
3	3.6902417425554135	1.054476236516905
4	1.6904796521233427	0.6440664309957599
5	0.9093432374205316	0.4330704696296744
6	0.6198865964392868	0.3542617155633441
7	0.3832987483313287	0.25556193411286016
8	0.3264647959925455	0.24876373488030135
9	0.26698740401009796	0.22887270749614771
>10	6.933742185331554	19.403319535506743
>50	4.294267701132714	28.49339315748658
>100	2.0103358490067276	32.628209001822924
>500	0.06344255144794407	4.145894391233848
>1k	0.015860637861986018	2.802998257646715
>5k	0.0013217198218321681	0.9120917303683113
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7245	0.9120917303683113	No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4292	0.5403309464100473	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2988	0.37616702420158926	No Hit
ATTCCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	2538	0.3195153639302656	No Hit
ATTCCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	2013	0.25342176028038793	No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1802	0.2268584262420562	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1511	0.19022368593326686	No Hit
ATTCCAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	1426	0.17952281677090573	No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1249	0.1572398303975184	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1219	0.15346305304609684	No Hit
ATTCCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1120	0.14099968778640562	No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1100	0.13848183621879123	No Hit
ATTCCAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA	1007	0.12677382642938434	No Hit
ATTCCAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	1000	0.1258925783807193	No Hit
ATTCCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	941	0.11846491625625685	No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	925	0.11645063500216535	No Hit
ATTCCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	920	0.11582117211026176	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	910	0.11456224632645456	No Hit
ATTCCAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT	905	0.11393278343455096	No Hit
ATTCCAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	877	0.11040779123989083	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	858	0.10801583225065717	No Hit
ATTCCAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	798	0.10046227754781399	No Hit
ATTCCAGGGGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATC	796	0.10021049239105255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.03638295515202788	0.0	0.0	0.0	0.0
7	0.04192222860077953	0.0	0.0	0.0	0.0
8	0.04733560947115046	0.0	0.0	0.0	0.0
9	0.05577041222265865	0.0	0.0	0.0	0.0
10	0.06898913295263417	0.0	0.0	0.0	0.0
11	0.08170428336908682	0.0	0.0	0.0	0.0
12	0.0906426564341179	0.0	0.0	0.0	0.0
13	0.10033638496943328	0.0	0.0	0.0	0.0
14	0.11519170921835815	0.0	0.0	0.0	0.0
15	0.13092828151594807	0.0	0.0	0.0	0.0
16	0.14515414287296935	0.0	0.0	0.0	0.0
17	0.15824697102456417	0.0	0.0	0.0	0.0
18	0.17008087339235178	0.0	0.0	0.0	0.0
19	0.18770583436565247	0.0	0.0	0.0	0.0
20	0.20105044767400873	0.0	0.0	0.0	0.0
21	0.21162542425798914	0.0	0.0	0.0	0.0
22	0.2263548559285333	0.0	0.0	0.0	0.0
23	0.24158785791260035	0.0	0.0	0.0	0.0
24	0.2553101489560987	0.0	0.0	0.0	0.0
25	0.2681511919509321	0.0	0.0	0.0	0.0
26	0.2840136568269027	0.0	0.0	0.0	0.0
27	0.29458863341088315	0.0	0.0	0.0	0.0
28	0.31284305727608747	0.0	0.0	0.0	0.0
29	0.3265653483195859	0.0	0.0	0.0	0.0
30	0.33940639131441924	0.0	0.0	0.0	0.0
31	0.3521215417308719	0.0	0.0	0.0	0.0
32	0.36546615503922814	0.0	0.0	0.0	0.0
33	0.37843309061244224	0.0	0.0	0.0	0.0
34	0.39366609259650925	0.0	0.0	0.0	0.0
35	0.4101580203643835	0.0	0.0	0.0	0.0
36	0.4285383368079685	0.0	0.0	0.0	0.0
37	0.4415052723811826	0.0	0.0	0.0	0.0
38	0.45384274506249306	0.0	0.0	2.517851567614386E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTTA	30	2.5267254E-6	44.0	12
CTTAAGG	25	4.4415938E-5	44.0	20
AACCGTT	35	1.4453326E-7	44.0	26
CTATGCG	20	7.855011E-4	44.0	31
ACACGTT	25	4.4415938E-5	44.0	20
ACTGCGT	35	1.4453326E-7	44.0	33
ACGCCGT	20	7.855011E-4	44.0	18
GCGACGT	30	2.5267254E-6	44.0	43
CGACGTT	30	2.5267254E-6	44.0	44
CGAAAAT	20	7.855011E-4	44.0	27
TAGACGG	20	7.855011E-4	44.0	30
TAGCGTT	20	7.855011E-4	44.0	42
ACCGATA	20	7.855011E-4	44.0	10
ATAGCGT	45	4.802132E-10	44.0	25
ATTCGTT	35	1.4453326E-7	44.0	14
TAACGTC	20	7.855011E-4	44.0	22
TAACGGC	25	4.4415938E-5	44.0	13
ATCGTTT	25	4.4415938E-5	44.0	17
AACGGTA	25	4.4415938E-5	44.0	44
TACATAC	20	7.855011E-4	44.0	28
>>END_MODULE