FastQCFastQC Report
Fri 17 Jun 2016
SRR1527656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009152
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC95140.9427717529173008No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA54160.5366882293252156No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT37140.3680317732115677No Hit
ATTCCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA31950.3166024543378995No Hit
ATTCCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT26590.26348855276509386No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT22200.2199866818873668No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC19380.19204242770167426No Hit
ATTCCAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA18780.1860968417047184No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG16150.1600353564180619No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC15480.15339611872146117No Hit
ATTCCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA14490.143585901826484No Hit
ATTCCAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG13740.1361539193302892No Hit
ATTCCAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA12780.1266409817351598No Hit
ATTCCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC12550.12436184043632674No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12210.12099267503805175No Hit
ATTCCAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG12110.12000174403855911No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT11870.11762350963977677No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT11680.11574074074074073No Hit
ATTCCAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT11390.11286704084221208No Hit
ATTCCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG11270.1116779236428209No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC11070.10969606164383562No Hit
ATTCCAGGGGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATCAA10540.1044441273465246No Hit
ATTCCAGGGGGTCTGGTAATTAATTAAATGACTTGTTCATGGAAAAAAAATA10480.10384956874682902No Hit
ATTCCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT10340.10246226534753931No Hit
ATTCCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT10310.10216498604769153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA206.3110865E-446.040
AACCGTA206.3110865E-446.045
AAACTCG253.416857E-546.020
CGAACTA301.8611754E-646.022
CGTATAT206.3110865E-446.043
ATCTACG253.416857E-546.041
CGACGTC206.3110865E-446.016
GCGAATC206.3110865E-446.046
ACGTTAT206.3110865E-446.041
ATGTGCG206.3110865E-446.037
CGCATAC301.8611754E-646.014
AACCACG206.3110865E-446.021
CCGGATA405.6097633E-946.014
TTCGTAA206.3110865E-446.022
CGTCTAA206.3110865E-446.010
CGATTAT253.416857E-546.046
CGACAGT253.416857E-546.034
TAACGGC501.6370905E-1146.013
CGACACA253.416857E-546.041
TAACGAC206.3110865E-446.030
Produced by FastQC (version 0.11.5)