##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009152
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.112725337709286	33.0	31.0	34.0	31.0	34.0
2	32.633327784119736	34.0	31.0	34.0	31.0	34.0
3	33.01138381532217	34.0	31.0	34.0	31.0	34.0
4	36.12552122970573	37.0	37.0	37.0	35.0	37.0
5	36.25908782819635	37.0	37.0	37.0	35.0	37.0
6	36.17232983732877	37.0	37.0	37.0	35.0	37.0
7	36.461811501141554	37.0	37.0	37.0	35.0	37.0
8	36.597457072869105	37.0	37.0	37.0	35.0	37.0
9	38.66785082921106	39.0	39.0	39.0	38.0	39.0
10	38.070006302321154	39.0	38.0	39.0	37.0	39.0
11	37.759025399543376	39.0	38.0	39.0	35.0	39.0
12	37.37073998763318	39.0	37.0	39.0	35.0	39.0
13	37.23971413622527	39.0	37.0	39.0	34.0	39.0
14	37.971371012493655	40.0	38.0	40.0	34.0	40.0
15	38.091055658612376	40.0	38.0	40.0	34.0	40.0
16	38.12873481893709	40.0	38.0	40.0	34.0	40.0
17	38.03699938165906	40.0	38.0	40.0	34.0	40.0
18	38.05346568207762	40.0	38.0	40.0	34.0	40.0
19	38.065062547564686	40.0	38.0	40.0	34.0	40.0
20	38.04205511161847	40.0	38.0	40.0	34.0	40.0
21	38.00972301496702	40.0	38.0	40.0	34.0	40.0
22	37.984106457699134	40.0	38.0	40.0	34.0	40.0
23	37.94636982337646	40.0	37.0	40.0	34.0	40.0
24	37.90867480815576	40.0	37.0	40.0	34.0	40.0
25	37.83922045440132	40.0	37.0	40.0	34.0	40.0
26	37.714930951927954	40.0	37.0	40.0	34.0	40.0
27	37.62593246607052	40.0	37.0	40.0	33.0	40.0
28	37.525533319063925	40.0	36.0	40.0	33.0	40.0
29	37.487952260908166	40.0	36.0	40.0	33.0	40.0
30	37.3550694048072	40.0	36.0	40.0	33.0	40.0
31	37.25033097095383	40.0	36.0	40.0	33.0	40.0
32	37.177389531012174	40.0	35.0	40.0	33.0	40.0
33	37.070435375443935	39.0	35.0	40.0	32.0	40.0
34	36.851592227929984	39.0	35.0	40.0	31.0	40.0
35	36.78799427638255	39.0	35.0	40.0	31.0	40.0
36	36.82720541603247	39.0	35.0	40.0	31.0	40.0
37	36.743509401953325	39.0	35.0	40.0	31.0	40.0
38	36.62761605783866	39.0	35.0	40.0	31.0	40.0
39	36.515129534500254	39.0	35.0	40.0	31.0	40.0
40	36.30972440227042	39.0	35.0	40.0	31.0	40.0
41	36.141215594875696	38.0	35.0	40.0	30.0	40.0
42	36.131468797564686	38.0	35.0	40.0	30.0	40.0
43	36.00138829433029	38.0	35.0	40.0	30.0	40.0
44	35.84861844400051	38.0	35.0	40.0	30.0	40.0
45	35.71807121226535	38.0	35.0	40.0	29.0	40.0
46	35.603467069381026	38.0	34.0	40.0	29.0	40.0
47	35.418259092782854	38.0	34.0	40.0	29.0	40.0
48	35.24290988869863	37.0	34.0	40.0	28.0	40.0
49	35.530583103437344	37.0	34.0	40.0	29.0	40.0
50	35.563838747780316	37.0	34.0	40.0	30.0	40.0
51	35.57097246004566	37.0	35.0	40.0	30.0	40.0
52	35.133518042871636	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	17.0
17	36.0
18	118.0
19	241.0
20	462.0
21	779.0
22	1248.0
23	2141.0
24	3038.0
25	4184.0
26	5666.0
27	7433.0
28	9683.0
29	12370.0
30	15720.0
31	20216.0
32	27207.0
33	37040.0
34	65723.0
35	74950.0
36	87628.0
37	145537.0
38	315946.0
39	171760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.39008196981229	0.5254907090309487	0.022295947488584475	0.06213137366818873
8	98.79671248731609	0.978445268899036	0.16657550101471336	0.058266742770167426
9	97.73225440766109	1.5398076801116183	0.3371147260273973	0.39082318619989853
10	58.00929889649924	26.322694698122778	6.586520167427702	9.081486237950278
11	33.06271007737189	23.94634306823947	21.962895579654997	21.028051274733638
12	29.726047215880264	17.100000792744797	29.03467465753425	24.13927733384069
13	23.340289668949772	19.22435866945713	28.347265823186202	29.0880858384069
14	23.143391679350582	19.42601312785388	33.33868436073059	24.09191083206494
15	24.923599219939117	20.341732464485034	30.876319920091323	23.858348395484523
16	27.98914335996956	19.507963121511924	26.75384877600203	25.749044742516492
17	27.415196125063417	19.737760020294267	25.009116565195335	27.837927289446977
18	28.004205511161846	22.2658231861999	23.942379344241502	25.787591958396757
19	29.745964928970064	22.217961218924405	22.195863457635717	25.840210394469814
20	29.495953037798074	23.047568651699645	22.713129439370878	24.743348871131403
21	28.780401763064432	21.223561960933537	26.050089580162357	23.945946695839677
22	27.595743753170982	20.41208856544901	26.24451024226281	25.747657439117198
23	28.26293759512938	22.347277714358192	24.88802479705733	24.501759893455098
24	28.75998858447489	21.55958666286149	23.594364377219684	26.086060375443935
25	26.765739947995943	23.155282851344495	23.713177004058853	26.36580019660071
26	27.140510052004057	22.010162988330798	23.632317034500254	27.21700992516489
27	25.016944920091323	21.5453172564688	24.172968987823438	29.26476883561644
28	25.736360825722983	23.679485350076103	22.50830400177575	28.075849822425162
29	28.408009893455098	22.19715166793506	22.618891901319127	26.775946537290718
30	27.809190290461693	21.17312357305936	24.901204179350582	26.11648195712836
31	28.861261732623035	22.004712867833586	23.559087233637747	25.574938165905632
32	32.14837804414003	21.75777286276002	22.634152238711312	23.459696854388636
33	31.649047913495686	20.777940290461693	23.15746289954338	24.41554889649924
34	26.927261700913242	23.270924498985288	26.177919679096906	23.623894121004565
35	27.640038368848302	25.35138413242009	24.979190449010655	22.029387049720953
36	28.39552416286149	25.5656234145104	22.477981513191274	23.560870909436833
37	29.24782391552511	25.313332382039572	21.580396213850836	23.858447488584474
38	28.886629566210047	25.0	22.792998477929984	23.32037195585997
39	28.032447044647384	23.29341863267377	21.962102834855404	26.712031487823438
40	28.29623287671233	23.37943144342973	22.75871226534754	25.5656234145104
41	25.272704211060375	25.659266393962454	23.26943810248605	25.798591292491118
42	25.28390173135464	23.56255549213597	23.31472364916286	27.838819127346525
43	26.573003868594625	22.05128662480974	23.804838121511924	27.570871385083713
44	26.11331097792998	21.378345383054288	23.953081399036023	28.555262239979704
45	27.21651445966514	22.385329464738714	22.821339104515477	27.57681697108067
46	25.90630549213597	24.944210584728566	22.55210315195332	26.597380771182138
47	25.37813926940639	23.83902524099442	24.914680840943685	25.868154648655505
48	27.057866406646376	22.312000570776256	24.982955986808726	25.64717703576865
49	27.739230561897514	24.282962328767123	22.99792300862506	24.9798841007103
50	24.729971302638255	24.139772799340438	24.464401794774226	26.66585410324708
51	24.694495972856416	23.917705162354135	25.646087011669206	25.741711853120243
52	25.297972951547436	24.720755644342972	24.24312690258752	25.73814450152207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	131.5
4	263.0
5	198.0
6	133.0
7	102.0
8	71.0
9	212.0
10	353.0
11	901.5
12	1450.0
13	1755.0
14	1918.0
15	1776.0
16	1894.5
17	2013.0
18	2143.5
19	2274.0
20	2089.5
21	1905.0
22	2259.0
23	2613.0
24	3820.0
25	5027.0
26	5056.0
27	5085.0
28	6819.0
29	8553.0
30	9047.5
31	9542.0
32	9747.5
33	9953.0
34	13599.0
35	17245.0
36	18131.0
37	19017.0
38	20413.5
39	23489.5
40	25169.0
41	27398.5
42	29628.0
43	33389.0
44	37150.0
45	37454.5
46	37759.0
47	42559.0
48	47359.0
49	50484.0
50	53609.0
51	58681.0
52	63753.0
53	65925.0
54	68097.0
55	72837.5
56	77578.0
57	84679.5
58	91781.0
59	88953.0
60	86125.0
61	85798.0
62	85471.0
63	70927.0
64	50484.0
65	44585.0
66	37383.0
67	30181.0
68	26052.0
69	21923.0
70	19608.5
71	17294.0
72	14692.0
73	12090.0
74	9253.0
75	6416.0
76	4687.0
77	2958.0
78	2257.5
79	1557.0
80	1269.0
81	981.0
82	551.5
83	122.0
84	75.0
85	28.0
86	19.0
87	10.0
88	6.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1009152.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.085600583460174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.01436344918869	5.499369767884323
2	9.533555566449335	1.5416904490106544
3	4.293102603068777	1.0413693873668188
4	2.2010882886416003	0.711884830035515
5	1.2647678807784697	0.511320395738204
6	0.7586156184126673	0.3680317732115677
7	0.4595813520270602	0.2601193873668189
8	0.3541840286288544	0.22910324708269914
9	0.23898230305407128	0.1739083904109589
>10	5.2674150693661455	12.202918886352105
>50	4.625226726800333	27.0544972412481
>100	2.873915388009216	40.65334062658549
>500	0.08333741850090691	4.561651763064434
>1k	0.02941320652973185	3.7113338723997966
>5k	0.0024511005441443206	1.4794599822425165
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9514	0.9427717529173008	No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5416	0.5366882293252156	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3714	0.3680317732115677	No Hit
ATTCCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	3195	0.3166024543378995	No Hit
ATTCCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	2659	0.26348855276509386	No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2220	0.2199866818873668	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1938	0.19204242770167426	No Hit
ATTCCAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1878	0.1860968417047184	No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1615	0.1600353564180619	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1548	0.15339611872146117	No Hit
ATTCCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1449	0.143585901826484	No Hit
ATTCCAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG	1374	0.1361539193302892	No Hit
ATTCCAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	1278	0.1266409817351598	No Hit
ATTCCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC	1255	0.12436184043632674	No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1221	0.12099267503805175	No Hit
ATTCCAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1211	0.12000174403855911	No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1187	0.11762350963977677	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	1168	0.11574074074074073	No Hit
ATTCCAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	1139	0.11286704084221208	No Hit
ATTCCAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1127	0.1116779236428209	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1107	0.10969606164383562	No Hit
ATTCCAGGGGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATCAA	1054	0.1044441273465246	No Hit
ATTCCAGGGGGTCTGGTAATTAATTAAATGACTTGTTCATGGAAAAAAAATA	1048	0.10384956874682902	No Hit
ATTCCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1034	0.10246226534753931	No Hit
ATTCCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1031	0.10216498604769153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.029926116184677828	0.0	0.0	0.0	0.0
7	0.03408802638254693	0.0	0.0	0.0	0.0
8	0.038448122780314564	0.0	0.0	0.0	0.0
9	0.04350187087772704	0.0	0.0	0.0	0.0
10	0.05351027397260274	0.0	0.0	0.0	0.0
11	0.06569872526636225	0.0	0.0	0.0	0.0
12	0.07422073186199898	0.0	0.0	0.0	0.0
13	0.08234636605783865	0.0	0.0	0.0	0.0
14	0.09691305175038052	0.0	0.0	0.0	0.0
15	0.11098427194317605	0.0	0.0	0.0	0.0
16	0.12376728183663115	0.0	0.0	0.0	0.0
17	0.13357749873160832	0.0	0.0	0.0	0.0
18	0.1461623224251649	0.0	0.0	0.0	0.0
19	0.16181903221714866	0.0	0.0	9.909309994926433E-5	0.0
20	0.17569206621004566	0.0	0.0	9.909309994926433E-5	0.0
21	0.18659230720446474	0.0	0.0	9.909309994926433E-5	0.0
22	0.1985825722983257	0.0	0.0	9.909309994926433E-5	0.0
23	0.21334744419076612	0.0	0.0	9.909309994926433E-5	0.0
24	0.22632864028411973	0.0	0.0	9.909309994926433E-5	0.0
25	0.24079623287671234	0.0	0.0	9.909309994926433E-5	0.0
26	0.25486745306950787	0.0	0.0	9.909309994926433E-5	0.0
27	0.26596588026382545	0.0	0.0	9.909309994926433E-5	0.0
28	0.28350535895484524	0.0	0.0	9.909309994926433E-5	0.0
29	0.2969820205479452	0.0	0.0	9.909309994926433E-5	0.0
30	0.31105324074074076	0.0	0.0	9.909309994926433E-5	0.0
31	0.32264713343480467	0.0	0.0	9.909309994926433E-5	0.0
32	0.3356283295281583	0.0	0.0	9.909309994926433E-5	0.0
33	0.3451412671232877	0.0	0.0	9.909309994926433E-5	0.0
34	0.3584197425164891	0.0	0.0	9.909309994926433E-5	0.0
35	0.3772474315068493	0.0	0.0	9.909309994926433E-5	0.0
36	0.39082318619989853	0.0	0.0	9.909309994926433E-5	0.0
37	0.40380438229325216	0.0	0.0	9.909309994926433E-5	0.0
38	0.4161910197869102	0.0	0.0	9.909309994926433E-5	0.0
39	0.42857765728056824	0.0	0.0	9.909309994926433E-5	0.0
40	0.4386851534753932	0.0	0.0	9.909309994926433E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	20	6.3110865E-4	46.0	40
AACCGTA	20	6.3110865E-4	46.0	45
AAACTCG	25	3.416857E-5	46.0	20
CGAACTA	30	1.8611754E-6	46.0	22
CGTATAT	20	6.3110865E-4	46.0	43
ATCTACG	25	3.416857E-5	46.0	41
CGACGTC	20	6.3110865E-4	46.0	16
GCGAATC	20	6.3110865E-4	46.0	46
ACGTTAT	20	6.3110865E-4	46.0	41
ATGTGCG	20	6.3110865E-4	46.0	37
CGCATAC	30	1.8611754E-6	46.0	14
AACCACG	20	6.3110865E-4	46.0	21
CCGGATA	40	5.6097633E-9	46.0	14
TTCGTAA	20	6.3110865E-4	46.0	22
CGTCTAA	20	6.3110865E-4	46.0	10
CGATTAT	25	3.416857E-5	46.0	46
CGACAGT	25	3.416857E-5	46.0	34
TAACGGC	50	1.6370905E-11	46.0	13
CGACACA	25	3.416857E-5	46.0	41
TAACGAC	20	6.3110865E-4	46.0	30
>>END_MODULE