FastQCFastQC Report
Fri 17 Jun 2016
SRR1527655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015742
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC89840.8844765698376161No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA50530.4974688454351597No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT34660.34122838279799395No Hit
ATTCCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA31080.30598321227240777No Hit
ATTCCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT24420.24041538107117752No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT22120.21777183576144338No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC18970.18676002370680744No Hit
ATTCCAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA17630.17356769730896232No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG15130.14895514805925125No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC14690.14462333939130212No Hit
ATTCCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA13870.1365504232373969No Hit
ATTCCAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG12750.12552400117352636No Hit
ATTCCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC12270.12079839171758183No Hit
ATTCCAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA12090.11902628817160263No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT11660.11479292970065233No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11590.11410377832166041No Hit
ATTCCAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT11420.11243012497268007No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT11400.11223322457868239No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC10700.1053417107887633No Hit
ATTCCAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG10500.1033727068487864No Hit
ATTCCAGGGGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATCAA10490.10327425665178758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT351.0194162E-746.00000442
GCGATTA351.0194162E-746.00000445
TCGTAAC351.0194162E-746.00000428
ATCGCCC351.0194162E-746.00000422
GATACGA405.6097633E-946.010
ATCTAGT253.416874E-546.019
ACGCATC206.311107E-446.031
ACGTTAT206.311107E-446.041
TCATTCG206.311107E-446.011
GACGTTA301.8611881E-646.035
CGTGATA206.311107E-446.044
CGGTTAC206.311107E-446.033
CGTTGCG206.311107E-446.021
TTATGCG405.6097633E-946.025
CCGGATA206.311107E-446.014
ATAGCGT750.046.025
ACCGAAT206.311107E-446.035
CGATTGA206.311107E-446.025
TCGATCT206.311107E-446.032
CGTTATA206.311107E-446.042
Produced by FastQC (version 0.11.5)