##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1015742
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06823976954778	33.0	31.0	34.0	31.0	34.0
2	32.60850688462228	34.0	31.0	34.0	31.0	34.0
3	32.99766476132719	34.0	31.0	34.0	31.0	34.0
4	36.106658974424604	37.0	37.0	37.0	35.0	37.0
5	36.25483636592757	37.0	37.0	37.0	35.0	37.0
6	36.09202139913482	37.0	36.0	37.0	35.0	37.0
7	36.41100495992092	37.0	36.0	37.0	35.0	37.0
8	36.56246468099182	37.0	37.0	37.0	35.0	37.0
9	38.61248821058891	39.0	39.0	39.0	38.0	39.0
10	37.972523534519596	39.0	38.0	39.0	35.0	39.0
11	37.714439296593035	39.0	38.0	39.0	35.0	39.0
12	37.31278808988897	39.0	37.0	39.0	35.0	39.0
13	37.04256494267245	39.0	37.0	39.0	34.0	39.0
14	37.912969041351054	40.0	38.0	40.0	33.0	40.0
15	38.04242908140059	40.0	38.0	40.0	34.0	40.0
16	38.109541596192734	40.0	38.0	40.0	34.0	40.0
17	38.048075200198475	40.0	38.0	40.0	34.0	40.0
18	38.07913229934373	40.0	38.0	40.0	34.0	40.0
19	38.04921525347972	40.0	38.0	40.0	34.0	40.0
20	38.036970017977005	40.0	38.0	40.0	34.0	40.0
21	38.05715723087162	40.0	38.0	40.0	34.0	40.0
22	37.921023251967526	40.0	38.0	40.0	34.0	40.0
23	37.909566602542775	40.0	37.0	40.0	34.0	40.0
24	37.91742785077313	40.0	37.0	40.0	34.0	40.0
25	37.82446428325303	40.0	37.0	40.0	34.0	40.0
26	37.6539396815333	40.0	37.0	40.0	34.0	40.0
27	37.563318244199806	40.0	37.0	40.0	33.0	40.0
28	37.47849847697545	40.0	36.0	40.0	33.0	40.0
29	37.44057349208756	40.0	36.0	40.0	33.0	40.0
30	37.29507689944887	40.0	36.0	40.0	33.0	40.0
31	37.20383424137232	40.0	36.0	40.0	32.0	40.0
32	37.13594889253373	40.0	35.0	40.0	32.0	40.0
33	36.87801626791055	39.0	35.0	40.0	32.0	40.0
34	36.75825258776343	39.0	35.0	40.0	31.0	40.0
35	36.760898929058754	39.0	35.0	40.0	31.0	40.0
36	36.65838273892386	39.0	35.0	40.0	31.0	40.0
37	36.64756700028157	39.0	35.0	40.0	31.0	40.0
38	36.27434230345895	39.0	35.0	40.0	30.0	40.0
39	36.32070348572768	39.0	35.0	40.0	30.0	40.0
40	36.18786168141123	39.0	35.0	40.0	30.0	40.0
41	35.82285363803013	38.0	35.0	40.0	29.0	40.0
42	35.96318947134213	38.0	35.0	40.0	30.0	40.0
43	35.94111299916711	38.0	35.0	40.0	30.0	40.0
44	35.79796542822882	38.0	35.0	40.0	30.0	40.0
45	35.666761835190435	38.0	35.0	40.0	29.0	40.0
46	35.381319272019866	38.0	34.0	40.0	28.0	40.0
47	35.11009291729593	37.0	34.0	40.0	27.0	40.0
48	35.10533285027103	37.0	34.0	40.0	27.0	40.0
49	35.05872061999996	37.0	34.0	40.0	28.0	40.0
50	34.877961135800234	36.0	34.0	40.0	27.0	40.0
51	35.18409891488193	37.0	34.0	40.0	28.0	40.0
52	34.939990666921325	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	6.0
15	9.0
16	18.0
17	57.0
18	132.0
19	234.0
20	484.0
21	796.0
22	1413.0
23	2187.0
24	3143.0
25	4363.0
26	5910.0
27	7792.0
28	10079.0
29	13042.0
30	16767.0
31	21752.0
32	28692.0
33	39529.0
34	68027.0
35	76097.0
36	94256.0
37	156075.0
38	315544.0
39	149336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.37336449610235	0.527594605716806	0.025203250431704117	0.07383764774913314
8	98.81889298660487	0.9565421140407702	0.17002349021700394	0.054541409137359685
9	97.64388988542366	1.5768768053304874	0.36387192810772817	0.4153613811381237
10	58.05568736943043	26.25381248387878	6.667638041943722	9.022862104747071
11	33.07749408806567	23.92014901421818	21.921610015141642	21.080746882574513
12	29.733337796408932	17.013276993567263	29.003329585662502	24.250055624361305
13	23.55942749241441	19.308643336595317	28.297638573574783	28.834290597415485
14	23.10143717597579	19.46931405809743	33.29762872855508	24.131620037371697
15	24.939108553156213	20.37820627679076	30.873883328640538	23.808801841412485
16	28.00337093474524	19.48299863548027	26.705600437906472	25.808029991868015
17	27.428618684665988	19.663950097564147	24.94284966064217	27.964581557127694
18	27.963006353975718	22.27859042945945	23.966715957398634	25.791687259166206
19	29.770059719889495	22.304778181861142	22.076964426005816	25.848197672243543
20	29.387974505336985	23.084897542879983	22.745638164021965	24.781489787761064
21	28.89030875950783	21.20449878020206	26.022749871522493	23.88244258876762
22	27.817201612220426	20.32632302297237	26.227723181674083	25.62875218313312
23	28.29301141431584	22.311472795257064	24.881219837320895	24.514295953106203
24	28.68198814265827	21.603025177653382	23.638286100210486	26.07670057947786
25	26.82689108060905	23.06146639599426	23.737720799179318	26.373921724217368
26	27.270015417300854	21.882820637524095	23.640353554347463	27.20681039082759
27	25.1023389797803	21.543758159060076	24.19748321916392	29.1564196419957
28	25.843865863575594	23.6031393798819	22.46013259272532	28.092862163817188
29	28.56187890231968	22.138495799130094	22.509554591618738	26.790070706931484
30	27.83954980693916	21.158817888794594	24.905438585782612	26.09619371848363
31	28.928605886140378	22.00086242372571	23.4666874068415	25.60384428329241
32	32.169094120357336	21.757099735956572	22.505025882556794	23.5687802611293
33	31.645929773505475	20.834326039486406	23.17783452884689	24.341909658161224
34	26.8656804582266	23.292529008350545	26.229495285220068	23.61229524820279
35	27.67966668701304	25.362247499857247	24.933103091139287	22.024982721990426
36	28.55567653990876	25.45223097991419	22.45550543346637	23.53658704671068
37	29.292871615036102	25.275217525710268	21.593967759529487	23.837943099724143
38	28.982556594095744	25.0167857585883	22.672391217454827	23.328266429861127
39	28.054761937578636	23.279828932937697	21.92938758070455	26.73602154877912
40	28.251957682167323	23.44630821606274	22.684992842670678	25.616741259099257
41	25.525970177466323	25.698750273199295	23.231588336408258	25.543691212926117
42	25.3664808583282	23.4665889566445	23.270673064616805	27.8962571204105
43	26.457013690484395	22.153460229073918	23.918081560081202	27.471444520360482
44	26.114603905322415	21.396082863561812	23.867182808232798	28.622130422882975
45	27.280451138182727	22.39259575758411	22.789743852277446	27.537209251955712
46	26.136361398859158	24.95643578782801	22.511228244967718	26.39597456834511
47	25.553339332232007	23.854187382228954	24.84410411305233	25.74836917248671
48	27.02398837500074	22.35036062307161	25.0255478261212	25.600103175806453
49	27.855794089443975	24.202996430195856	23.02218476739172	24.91902471296845
50	24.81584890651366	24.181042036265115	24.377450179277808	26.625658877943415
51	24.739057752854563	23.87308982005273	25.61565830693227	25.772194120160435
52	25.28900055329011	24.666696858060412	24.199550673300898	25.844751915348585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	125.0
4	250.0
5	202.5
6	155.0
7	122.5
8	90.0
9	196.5
10	303.0
11	790.0
12	1277.0
13	1699.0
14	1938.5
15	1756.0
16	1846.0
17	1936.0
18	2110.5
19	2285.0
20	2113.5
21	1942.0
22	2293.5
23	2645.0
24	3730.5
25	4816.0
26	5012.5
27	5209.0
28	6844.5
29	8480.0
30	8970.0
31	9460.0
32	9871.0
33	10282.0
34	13793.5
35	17305.0
36	18255.5
37	19206.0
38	20622.0
39	23487.0
40	24936.0
41	27460.0
42	29984.0
43	33449.0
44	36914.0
45	37744.0
46	38574.0
47	43236.0
48	47898.0
49	51017.0
50	54136.0
51	58826.5
52	63517.0
53	66406.0
54	69295.0
55	73512.5
56	77730.0
57	84842.0
58	91954.0
59	89311.0
60	86668.0
61	86159.0
62	85650.0
63	71421.5
64	50992.0
65	44791.0
66	37819.0
67	30847.0
68	26429.0
69	22011.0
70	19632.0
71	17253.0
72	14841.0
73	12429.0
74	9478.5
75	6528.0
76	4825.5
77	3123.0
78	2365.5
79	1608.0
80	1289.5
81	971.0
82	545.0
83	119.0
84	81.0
85	43.0
86	27.5
87	12.0
88	6.5
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1015742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.473590468858408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11269875304137	9.369250432561698
2	9.442268952204245	2.35557992013225
3	3.4198195010914647	1.2797228380209182
4	1.6057319760258475	0.8011697228678875
5	0.8762102343606575	0.5464743814018643
6	0.5224530380619262	0.39101191415972175
7	0.3330079839264414	0.2907663650051029
8	0.2528756502596726	0.25234138407083395
9	0.2057510552891469	0.23098089619910359
>10	3.542498982525472	12.74066607889629
>50	2.8946241214252475	25.850916552104163
>100	1.7237384553793862	37.08698723672803
>500	0.05025934448486594	4.220211961934423
>1k	0.016491347409096635	3.2089205408400003
>5k	0.0015706045151520605	1.3749997750777219
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8984	0.8844765698376161	No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5053	0.4974688454351597	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3466	0.34122838279799395	No Hit
ATTCCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	3108	0.30598321227240777	No Hit
ATTCCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	2442	0.24041538107117752	No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2212	0.21777183576144338	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1897	0.18676002370680744	No Hit
ATTCCAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1763	0.17356769730896232	No Hit
ATTCCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1513	0.14895514805925125	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1469	0.14462333939130212	No Hit
ATTCCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1387	0.1365504232373969	No Hit
ATTCCAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG	1275	0.12552400117352636	No Hit
ATTCCAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC	1227	0.12079839171758183	No Hit
ATTCCAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	1209	0.11902628817160263	No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1166	0.11479292970065233	No Hit
ATTCCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1159	0.11410377832166041	No Hit
ATTCCAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	1142	0.11243012497268007	No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	1140	0.11223322457868239	No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1070	0.1053417107887633	No Hit
ATTCCAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1050	0.1033727068487864	No Hit
ATTCCAGGGGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATCAA	1049	0.10327425665178758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.027270704568679843	0.0	0.0	0.0	0.0
7	0.03189786382762552	0.0	0.0	0.0	0.0
8	0.03632812269257351	0.0	0.0	0.0	0.0
9	0.042432034906501846	0.0	0.0	0.0	0.0
10	0.053261556576374706	0.0	0.0	0.0	0.0
11	0.06852133711119555	0.0	0.0	0.0	0.0
12	0.07698805405309617	0.0	0.0	0.0	0.0
13	0.08456871922200716	0.0	0.0	0.0	0.0
14	0.09776104561985229	0.0	0.0	0.0	0.0
15	0.1136115273366662	0.0	0.0	0.0	0.0
16	0.12641005294651594	0.0	0.0	0.0	0.0
17	0.13625507264640035	0.0	0.0	0.0	0.0
18	0.14816754648326053	0.0	0.0	0.0	0.0
19	0.16204902426009754	0.0	0.0	9.845019699884419E-5	0.0
20	0.17504445026394497	0.0	0.0	9.845019699884419E-5	0.0
21	0.18439721897883518	0.0	0.0	9.845019699884419E-5	0.0
22	0.19571899163370227	0.0	0.0	9.845019699884419E-5	0.0
23	0.2087144176375497	0.0	0.0	9.845019699884419E-5	0.0
24	0.2231865965963798	0.0	0.0	9.845019699884419E-5	0.0
25	0.23519752063023877	0.0	0.0	9.845019699884419E-5	0.0
26	0.24829139683108506	0.0	0.0	9.845019699884419E-5	0.0
27	0.26187752401692554	0.0	0.0	1.9690039399768839E-4	0.0
28	0.27930320888572097	0.0	0.0	1.9690039399768839E-4	0.0
29	0.2902311807525927	0.0	0.0	1.9690039399768839E-4	0.0
30	0.3023405549834505	0.0	0.0	1.9690039399768839E-4	0.0
31	0.31287472606232686	0.0	0.0	1.9690039399768839E-4	0.0
32	0.32518100068718236	0.0	0.0	1.9690039399768839E-4	0.0
33	0.3362074227510529	0.0	0.0	1.9690039399768839E-4	0.0
34	0.34831679698191076	0.0	0.0	1.9690039399768839E-4	0.0
35	0.365840932047705	0.0	0.0	1.9690039399768839E-4	0.0
36	0.3824790153405097	0.0	0.0	1.9690039399768839E-4	0.0
37	0.3951790907533606	0.0	0.0	1.9690039399768839E-4	0.0
38	0.40600861242323344	0.0	0.0	2.953505909965326E-4	0.0
39	0.4168381340931063	0.0	0.0	2.953505909965326E-4	0.0
40	0.4305227114759457	0.0	0.0	2.953505909965326E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	35	1.0194162E-7	46.000004	42
GCGATTA	35	1.0194162E-7	46.000004	45
TCGTAAC	35	1.0194162E-7	46.000004	28
ATCGCCC	35	1.0194162E-7	46.000004	22
GATACGA	40	5.6097633E-9	46.0	10
ATCTAGT	25	3.416874E-5	46.0	19
ACGCATC	20	6.311107E-4	46.0	31
ACGTTAT	20	6.311107E-4	46.0	41
TCATTCG	20	6.311107E-4	46.0	11
GACGTTA	30	1.8611881E-6	46.0	35
CGTGATA	20	6.311107E-4	46.0	44
CGGTTAC	20	6.311107E-4	46.0	33
CGTTGCG	20	6.311107E-4	46.0	21
TTATGCG	40	5.6097633E-9	46.0	25
CCGGATA	20	6.311107E-4	46.0	14
ATAGCGT	75	0.0	46.0	25
ACCGAAT	20	6.311107E-4	46.0	35
CGATTGA	20	6.311107E-4	46.0	25
TCGATCT	20	6.311107E-4	46.0	32
CGTTATA	20	6.311107E-4	46.0	42
>>END_MODULE