FastQCFastQC Report
Fri 17 Jun 2016
SRR1527654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805830
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC64010.7943362744003077No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG58430.7250909000657707No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC41020.5090403683158979No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT37690.4677165158904484No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT36050.4473648288100468No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT35690.4428973853045927No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC26500.3288534802625864No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT25730.319298114987032No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA23930.296960897459762No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA21670.26891527989774516No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG19130.2373949840537086No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC15360.19061092289937084No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA14660.1819242271943214No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG14620.18142784458260428No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12570.1559882357321023No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC11330.14060037476887186No Hit
CTGAAAGGGGACTTGCACTGCTCCATCCGTCCAACATGTTCAAGTTCTTC10730.1331546355931152No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG10550.13092091384038818No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC10200.12657756598786343No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC9990.1239715572763486No Hit
CTGAAAGGGGATCACGCTCAAACATTCTCACAATCTAACCAACCAAACCA9830.12198602682948016No Hit
CTGAAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCT9480.11764267897695543No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG9320.11565714853008699No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9180.11391980938907709No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG8830.10957646153655237No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT8780.10895598327190599No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA8760.10870779196604743No Hit
CTGAAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC8170.10138614844322003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG254.441652E-544.040
TAATACC207.85508E-444.026
CGTATCT254.441652E-544.014
TTTACGC302.526771E-644.044
CGCATTC254.441652E-544.032
ATATGTC254.441652E-544.041
CACGATA207.85508E-444.021
TATGCGG207.85508E-444.023
CCGCTAT302.526771E-644.021
TTATACG207.85508E-444.016
AATGCCG408.307325E-944.019
ATATCGT351.445369E-744.014
ACGATAC454.802132E-1044.043
CTAGTTT207.85508E-444.038
TAACAGG207.85508E-444.019
GTGTCGA254.441652E-544.021
TGTTACG302.526771E-644.029
GAACGTT207.85508E-444.039
AGATTCG254.441652E-544.044
TTAACTA351.445369E-744.023
Produced by FastQC (version 0.11.5)