##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	805830
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.520761202735066	33.0	31.0	34.0	31.0	34.0
2	32.907269523348596	34.0	31.0	34.0	31.0	34.0
3	33.20597892855813	34.0	34.0	34.0	31.0	34.0
4	36.22075747986548	37.0	35.0	37.0	35.0	37.0
5	36.47483091967288	37.0	37.0	37.0	35.0	37.0
6	36.56095206184927	37.0	37.0	37.0	35.0	37.0
7	36.5158271595746	37.0	37.0	37.0	35.0	37.0
8	36.667633371802985	37.0	37.0	37.0	35.0	37.0
9	38.78201109415137	39.0	39.0	39.0	39.0	39.0
10	38.34757579142003	39.0	39.0	39.0	37.0	39.0
11	38.12057009542956	39.0	39.0	39.0	37.0	39.0
12	37.55238077510145	39.0	37.0	39.0	35.0	39.0
13	37.427682017298935	39.0	37.0	39.0	35.0	39.0
14	38.156278619559956	40.0	38.0	40.0	35.0	40.0
15	38.20090962113597	40.0	38.0	40.0	35.0	40.0
16	38.29905190921162	40.0	38.0	40.0	35.0	40.0
17	38.1659096831838	40.0	38.0	40.0	34.0	40.0
18	38.09181216881973	40.0	38.0	40.0	34.0	40.0
19	38.08445950138367	40.0	38.0	40.0	34.0	40.0
20	37.984409863122494	40.0	38.0	40.0	34.0	40.0
21	37.94833649777248	40.0	37.0	40.0	34.0	40.0
22	38.037625801968154	40.0	38.0	40.0	34.0	40.0
23	37.975973840636364	40.0	37.0	40.0	34.0	40.0
24	37.92172294404527	40.0	37.0	40.0	34.0	40.0
25	37.93400841368527	40.0	37.0	40.0	34.0	40.0
26	37.82223049526575	40.0	37.0	40.0	34.0	40.0
27	37.71522901852748	40.0	37.0	40.0	34.0	40.0
28	37.60526537855379	40.0	36.0	40.0	34.0	40.0
29	37.66231587307497	40.0	37.0	40.0	34.0	40.0
30	37.72039884342851	40.0	37.0	40.0	34.0	40.0
31	37.77911842448159	40.0	37.0	40.0	34.0	40.0
32	37.7672908678009	40.0	36.0	40.0	34.0	40.0
33	37.691855602298254	40.0	36.0	40.0	34.0	40.0
34	37.293692217961606	40.0	35.0	40.0	33.0	40.0
35	37.32323070622836	40.0	35.0	40.0	33.0	40.0
36	37.333526922551904	40.0	35.0	40.0	33.0	40.0
37	37.28137944727796	40.0	35.0	40.0	33.0	40.0
38	37.17407021332043	40.0	35.0	40.0	33.0	40.0
39	37.067401312932006	40.0	35.0	40.0	33.0	40.0
40	36.919245994812805	39.0	35.0	40.0	33.0	40.0
41	36.771139073998235	39.0	35.0	40.0	33.0	40.0
42	36.72017919412283	39.0	35.0	40.0	33.0	40.0
43	36.64216025712619	39.0	35.0	40.0	32.0	40.0
44	36.31818373602373	39.0	35.0	40.0	31.0	40.0
45	36.2577801769604	38.0	35.0	40.0	31.0	40.0
46	36.13206135289081	38.0	35.0	40.0	31.0	40.0
47	36.04363947730911	38.0	35.0	40.0	31.0	40.0
48	35.94622563071616	37.0	35.0	40.0	31.0	40.0
49	35.83398979933733	37.0	35.0	40.0	31.0	40.0
50	35.345766476800314	36.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	10.0
15	7.0
16	34.0
17	55.0
18	107.0
19	182.0
20	297.0
21	537.0
22	760.0
23	1156.0
24	1588.0
25	2208.0
26	3050.0
27	4077.0
28	5420.0
29	7113.0
30	9430.0
31	12724.0
32	16366.0
33	23414.0
34	52640.0
35	64236.0
36	59939.0
37	110420.0
38	248329.0
39	181726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.70576920690468	0.17758087934179664	0.06676346127595151	0.04988645247756971
8	99.73480758969013	0.18651576635270464	0.06155144385292183	0.01712520010424035
9	99.60413486715561	0.19706389685169326	0.12173783552362162	0.07706340046908157
10	68.51792561706563	22.550413859002518	2.520382710993634	6.411277812938213
11	39.407691448568556	30.265936984227444	14.876214586203046	15.450156981000957
12	33.01577255748731	16.67299554496606	24.004566720027796	26.306665177518834
13	25.35534790216299	18.06311504907983	26.892024372386235	29.68951267637095
14	22.86847101746026	20.091954878820594	29.596316841021057	27.44325726269809
15	23.565144013005224	21.25981906853803	28.042515170693573	27.132521747763178
16	29.63354553689984	20.88157551840959	24.101113138006774	25.383765806683794
17	30.323269175880768	20.307384932305823	23.73130809227753	25.638037799535883
18	28.2507476763089	21.284886390429744	23.837782162490846	26.626583770770512
19	29.179355447178683	22.45262648449425	21.18225928545723	27.185758782869833
20	29.894270503704256	22.44245064095405	22.46280232803445	25.20047652730725
21	29.017410620105977	23.03686881848529	23.666157874489656	24.279562686919075
22	29.2268840822506	20.373403819664198	24.71005050693074	25.68966159115446
23	28.168348162763856	23.2140774108683	23.21482198478587	25.40275244158197
24	28.636933348224815	21.35450405172307	23.135648958216994	26.872913641835126
25	28.317014754973137	23.47480237767271	21.335641512477817	26.87254135487634
26	26.080066515269966	23.06801682737054	23.113435836342653	27.738480821016843
27	24.851767742575976	21.201742302967126	23.15736569747962	30.789124256977278
28	25.82691138329424	22.588883511410597	21.402032686794982	30.18217241850018
29	29.071268133477286	22.588883511410597	21.153965476589356	27.185882878522765
30	26.229849968355605	21.79901468051574	23.547894717248056	28.423240633880596
31	29.51590285792289	21.67702865368626	22.013327873124606	26.79374061526625
32	32.872193887048134	21.692292418996562	20.759465395927183	24.67604829802812
33	32.36489085787325	21.531836739759004	20.64790340394376	25.45536899842398
34	29.016293759229615	22.60849062457342	24.694166263355793	23.681049352841168
35	27.453681297544147	25.64722087785265	24.28067954779544	22.618418276807763
36	27.92760259608106	26.05326185423725	21.193800181179654	24.825335368502042
37	28.68247645284986	25.830137870270402	20.648275690902548	24.839109985977192
38	28.70903292257672	26.50087487435315	21.682364766762223	23.107727436307908
39	30.082399513545038	23.584502934862194	21.387389399749328	24.94570815184344
40	28.374098755320603	24.224712408324336	20.860106970452826	26.54108186590224
41	25.511956616159736	25.42856433739126	22.13270789124257	26.92677115520643
42	24.987404291227676	22.798108782249358	21.544494496357792	30.669992430165173
43	25.80345730489061	21.126292145986124	24.477743444647135	28.592507104476127
44	25.75208170457789	21.47611779159376	23.41622922949009	29.35557127433826
45	27.66241018577119	21.983793107727436	22.56282342429545	27.790973282205922
46	26.89289304195674	24.035962920218903	21.360212451757814	27.71093158606654
47	25.185833240261594	23.545660995495325	23.770025935991463	27.498479828251615
48	26.907412233349465	22.143256021741557	25.56271173820781	25.386620006701165
49	27.754489160244717	22.551158432920097	23.074842088281645	26.61951031855354
50	25.320228832384	23.52282739535634	24.61226313242247	26.544680639837186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	25.0
4	50.0
5	39.0
6	28.0
7	18.0
8	8.0
9	124.0
10	240.0
11	1092.5
12	1945.0
13	2313.5
14	2682.0
15	2312.0
16	1942.0
17	1822.5
18	1703.0
19	1743.5
20	1784.0
21	1831.0
22	1878.0
23	2104.0
24	2330.0
25	2475.5
26	2621.0
27	2963.0
28	3305.0
29	3958.5
30	4612.0
31	5392.5
32	6173.0
33	8601.5
34	11030.0
35	10713.5
36	10397.0
37	11305.5
38	12214.0
39	12766.5
40	13319.0
41	16516.0
42	19713.0
43	22734.5
44	25756.0
45	28475.0
46	31194.0
47	35431.5
48	39669.0
49	40349.0
50	41029.0
51	44660.0
52	48291.0
53	51955.5
54	55620.0
55	61966.0
56	68312.0
57	72765.0
58	77218.0
59	76327.0
60	75436.0
61	76756.0
62	78076.0
63	67639.5
64	57203.0
65	47135.0
66	37067.0
67	29360.5
68	21654.0
69	19694.0
70	17734.0
71	14987.0
72	12240.0
73	12036.0
74	11832.0
75	9025.0
76	6218.0
77	4035.0
78	1852.0
79	1435.5
80	1019.0
81	686.0
82	353.0
83	210.0
84	67.0
85	38.5
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	805830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.79192881873348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.6589317976513	5.948525123164935
2	9.249378952122854	1.6263976272911158
3	3.443992773261066	0.9083801794423141
4	1.6965898825654924	0.5966518992839681
5	0.9386291779584462	0.412618045989849
6	0.5349480578139115	0.2821935147611779
7	0.4149728997289973	0.2553888537284539
8	0.3571025293586269	0.2511696015288585
9	0.28935185185185186	0.2289564796545177
>10	8.856989611562781	22.92145986126106
>50	4.436257904245709	26.692850849434745
>100	2.022640018066847	29.359045952620278
>500	0.07339656729900633	4.437288261792189
>1k	0.023995031616982836	4.559646575580458
>5k	0.002822944896115628	1.5194271744660786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6401	0.7943362744003077	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5843	0.7250909000657707	No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4102	0.5090403683158979	No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	3769	0.4677165158904484	No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3605	0.4473648288100468	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	3569	0.4428973853045927	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2650	0.3288534802625864	No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2573	0.319298114987032	No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	2393	0.296960897459762	No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2167	0.26891527989774516	No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1913	0.2373949840537086	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1536	0.19061092289937084	No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	1466	0.1819242271943214	No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1462	0.18142784458260428	No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1257	0.1559882357321023	No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1133	0.14060037476887186	No Hit
CTGAAAGGGGACTTGCACTGCTCCATCCGTCCAACATGTTCAAGTTCTTC	1073	0.1331546355931152	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1055	0.13092091384038818	No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1020	0.12657756598786343	No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	999	0.1239715572763486	No Hit
CTGAAAGGGGATCACGCTCAAACATTCTCACAATCTAACCAACCAAACCA	983	0.12198602682948016	No Hit
CTGAAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCT	948	0.11764267897695543	No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	932	0.11565714853008699	No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	918	0.11391980938907709	No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	883	0.10957646153655237	No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	878	0.10895598327190599	No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	876	0.10870779196604743	No Hit
CTGAAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	817	0.10138614844322003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.02407455666827991	0.0	0.0	0.0	0.0
7	0.02419865232120919	0.0	0.0	0.0	0.0
8	0.03598773934949059	0.0	0.0	0.0	0.0
9	0.06887308737574922	0.0	0.0	0.0	0.0
10	0.1016343397490786	0.0	0.0	0.0	0.0
11	0.14568829653897225	0.0	0.0	0.0	0.0
12	0.16380626186664682	0.0	0.0	0.0	0.0
13	0.176836305424221	0.0	0.0	0.0	0.0
14	0.18564709678219973	0.0	0.0	0.0	0.0
15	0.19718799250462257	0.0	0.0	0.0	0.0
16	0.21021803606219674	0.0	0.0	0.0	0.0
17	0.22126254917290247	0.0	0.0	0.0	0.0
18	0.2340444014246181	0.0	0.0	0.0	0.0
19	0.2515418884876463	0.0	0.0	0.0	0.0
20	0.2659369842274425	0.0	0.0	0.0	0.0
21	0.2752441581971384	0.0	0.0	0.0	0.0
22	0.28591638434905625	0.0	0.0	0.0	0.0
23	0.2968368018068327	0.0	0.0	0.0	0.0
24	0.30837769752925553	0.0	0.0	0.0	0.0
25	0.3200426889046077	0.0	0.0	0.0	0.0
26	0.3302185324448085	0.0	0.0	0.0	0.0
27	0.34151123686137275	0.0	0.0	0.0	0.0
28	0.3564027152128861	0.0	0.0	0.0	0.0
29	0.36620627179429904	0.0	0.0	0.0	0.0
30	0.3758857327227827	0.0	0.0	0.0	0.0
31	0.3855651936512664	0.0	0.0	0.0	0.0
32	0.39871933286176986	0.0	0.0	0.0	0.0
33	0.4098879416254049	0.0	0.0	0.0	0.0
34	0.42217341126540336	0.0	0.0	0.0	0.0
35	0.43632031569934104	0.0	0.0	0.0	0.0
36	0.45034312448034947	0.0	0.0	0.0	0.0
37	0.4627526897732772	0.0	0.0	0.0	0.0
38	0.4745417768015586	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	25	4.441652E-5	44.0	40
TAATACC	20	7.85508E-4	44.0	26
CGTATCT	25	4.441652E-5	44.0	14
TTTACGC	30	2.526771E-6	44.0	44
CGCATTC	25	4.441652E-5	44.0	32
ATATGTC	25	4.441652E-5	44.0	41
CACGATA	20	7.85508E-4	44.0	21
TATGCGG	20	7.85508E-4	44.0	23
CCGCTAT	30	2.526771E-6	44.0	21
TTATACG	20	7.85508E-4	44.0	16
AATGCCG	40	8.307325E-9	44.0	19
ATATCGT	35	1.445369E-7	44.0	14
ACGATAC	45	4.802132E-10	44.0	43
CTAGTTT	20	7.85508E-4	44.0	38
TAACAGG	20	7.85508E-4	44.0	19
GTGTCGA	25	4.441652E-5	44.0	21
TGTTACG	30	2.526771E-6	44.0	29
GAACGTT	20	7.85508E-4	44.0	39
AGATTCG	25	4.441652E-5	44.0	44
TTAACTA	35	1.445369E-7	44.0	23
>>END_MODULE