FastQCFastQC Report
Fri 17 Jun 2016
SRR1527653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227169
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC96470.7861182934053909No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT81830.6668193215441394No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT63000.5133767231734179No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT57900.4718176551069983No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC55500.4522604466051538No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG54470.4438671446231122No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA40440.3295389632560796No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38830.3164193358860923No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG34270.27926063973258775No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33460.2726600818632153No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG29720.24218343194784092No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC25310.20624706132570167No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG23080.18807515509273784No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA21820.1778076206292695No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG18470.1505090170954449No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17520.14276762206346477No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT17500.1426046453259494No Hit
CTGAAAGGGGACTTGCACTGCTCCATCCGTCCAACATGTTCAAGTTCTTCGT16230.13225562249372336No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT15550.12671441341820075No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG15290.12459571583050093No Hit
CTGAAAGGGGATCACGCTCAAACATTCTCACAATCTAACCAACCAAACCAAC14840.12092873923640508No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC14800.12060278576137434No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT14650.11938046023000908No Hit
CTGAAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAA14500.1181581346986438No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT14320.11669134406100547No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG13590.11074269314169441No Hit
CTGAAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG13380.10903143739778302No Hit
CTGAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC12540.10218641442213745No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12300.100230693571953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC351.0196527E-746.00000443
CTCGATT351.0196527E-746.00000446
GTATACC351.0196527E-746.00000444
CGTATCT301.8615083E-646.014
CTCGTAC253.4172932E-546.044
CTACTAG206.3116214E-446.026
TAACGCG206.3116214E-446.020
AAACGAC301.8615083E-646.014
CTACGTA301.8615083E-646.046
CGTAGAT253.4172932E-546.043
TTGCGTA301.8615083E-646.013
TTGCGAT206.3116214E-446.011
AACGAAT301.8615083E-646.030
TCGTATA206.3116214E-446.033
CGTACGT301.8615083E-646.039
CGTTTAT206.3116214E-446.040
TCGGTTA206.3116214E-446.034
ACGAATT253.4172932E-546.015
TAATCGG301.8615083E-646.044
TACGCAT453.092282E-1045.99999646
Produced by FastQC (version 0.11.5)