##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1227169
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1964342319599	33.0	31.0	34.0	31.0	34.0
2	32.58527472581201	34.0	31.0	34.0	31.0	34.0
3	32.88232753597915	34.0	31.0	34.0	31.0	34.0
4	36.06011559939992	37.0	35.0	37.0	35.0	37.0
5	36.26956841315255	37.0	37.0	37.0	35.0	37.0
6	36.38565266886631	37.0	37.0	37.0	35.0	37.0
7	36.3947842554693	37.0	36.0	37.0	35.0	37.0
8	36.56169199189354	37.0	37.0	37.0	35.0	37.0
9	38.692754624668645	39.0	39.0	39.0	38.0	39.0
10	38.10240235859934	39.0	38.0	39.0	37.0	39.0
11	37.77060861217974	39.0	38.0	39.0	35.0	39.0
12	37.165745712285755	39.0	37.0	39.0	34.0	39.0
13	37.023200553469	39.0	37.0	39.0	33.0	39.0
14	37.788809854225455	40.0	37.0	40.0	33.0	40.0
15	37.85463208408948	40.0	37.0	40.0	33.0	40.0
16	37.95345710330036	40.0	37.0	40.0	34.0	40.0
17	37.825003728092874	40.0	37.0	40.0	33.0	40.0
18	37.81239014349287	40.0	37.0	40.0	33.0	40.0
19	37.81614594240891	40.0	37.0	40.0	34.0	40.0
20	37.783689125132724	40.0	37.0	40.0	33.0	40.0
21	37.73371719787576	40.0	37.0	40.0	33.0	40.0
22	37.76262519669255	40.0	37.0	40.0	34.0	40.0
23	37.72298436482669	40.0	37.0	40.0	34.0	40.0
24	37.672620478516	40.0	36.0	40.0	34.0	40.0
25	37.67227741248353	40.0	36.0	40.0	34.0	40.0
26	37.54936117193312	40.0	36.0	40.0	33.0	40.0
27	37.44870836861101	40.0	36.0	40.0	33.0	40.0
28	37.38578060560526	40.0	35.0	40.0	33.0	40.0
29	37.312053189088054	40.0	35.0	40.0	33.0	40.0
30	37.13645471813581	40.0	35.0	40.0	32.0	40.0
31	37.0772631968376	39.0	35.0	40.0	32.0	40.0
32	36.96885025615869	39.0	35.0	40.0	32.0	40.0
33	36.86787720354735	39.0	35.0	40.0	32.0	40.0
34	36.59867222851946	39.0	35.0	40.0	31.0	40.0
35	36.519729556401764	39.0	35.0	40.0	31.0	40.0
36	36.57250957284612	39.0	35.0	40.0	31.0	40.0
37	36.53855336958479	39.0	35.0	40.0	31.0	40.0
38	36.43100339073102	38.0	35.0	40.0	31.0	40.0
39	36.323289620256055	38.0	35.0	40.0	31.0	40.0
40	36.143534427613474	38.0	35.0	40.0	30.0	40.0
41	35.97980473757078	38.0	35.0	40.0	30.0	40.0
42	35.93398790223677	38.0	35.0	40.0	30.0	40.0
43	35.84341439524629	38.0	35.0	40.0	30.0	40.0
44	35.64257979137348	37.0	34.0	40.0	30.0	40.0
45	35.513749939902326	37.0	34.0	40.0	29.0	40.0
46	35.385985956294526	37.0	34.0	40.0	29.0	40.0
47	35.20464174046118	37.0	34.0	40.0	29.0	40.0
48	35.009343456361755	36.0	34.0	40.0	28.0	40.0
49	35.307651187407764	36.0	34.0	40.0	29.0	40.0
50	35.353786642263614	36.0	34.0	40.0	30.0	40.0
51	35.32069014129268	36.0	34.0	40.0	30.0	40.0
52	34.8560410179853	35.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	3.0
16	21.0
17	77.0
18	158.0
19	306.0
20	548.0
21	1008.0
22	1542.0
23	2380.0
24	3616.0
25	5045.0
26	6898.0
27	9247.0
28	12320.0
29	15398.0
30	19909.0
31	25705.0
32	34207.0
33	49678.0
34	97167.0
35	98865.0
36	116805.0
37	186584.0
38	365686.0
39	173991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.75553489372695	0.17283683013505066	0.043270323810330934	0.028357952327674508
8	99.77150661400344	0.1549093890083599	0.058345672030502733	0.015238324957687165
9	99.62898345704626	0.18416371339236892	0.10674976307256784	0.08010306648880472
10	68.66853709635755	22.47856652180751	2.4225677147972284	6.43032866703771
11	39.398566945547024	30.225502762863144	14.881650367634775	15.494279923955053
12	33.03954060117229	16.641962109538294	24.158204778640922	26.160292510648492
13	25.37433719398062	17.987824007940226	26.90819275910653	29.72964603897263
14	22.897416737222013	19.955849601807085	29.61132492753647	27.535408733434437
15	23.58257094173663	21.21704508506978	27.994514203015232	27.205869770178353
16	29.66722594850424	20.82777514751432	24.10189631582936	25.40310258815208
17	30.38717568647839	20.251652380397484	23.73682842379493	25.624343509329194
18	28.281434749411044	21.275146291994012	23.858898000193943	26.584520958401004
19	29.107237878401428	22.465365406068766	21.215822759538415	27.21157395599139
20	29.95439096000632	22.34484410867615	22.53593433341292	25.164830597904608
21	28.939616303866867	23.016878685820778	23.678645728501944	24.36485928181041
22	29.37704586735812	20.297122890164275	24.657321037281744	25.66851020519586
23	28.36667158313158	23.12770286733123	23.169506400503927	25.336119149033266
24	28.66711919874117	21.14427597176917	23.081743427351896	27.106861402137767
25	28.306777632094683	23.33126081248793	21.31857959254186	27.04338196287553
26	26.123378279601262	22.98134975704243	23.205442771126066	27.68982919223025
27	24.951657025234503	20.87813495940657	23.22768909579691	30.94251891956202
28	25.87206815035256	22.39447052524958	21.415061821150957	30.31839950324691
29	29.083687739830456	22.419813407933216	21.284924896244934	27.21157395599139
30	26.268753529464973	21.658549066998923	23.60685447562642	28.465842927909684
31	29.645631530783454	21.509425352172357	22.030869423852785	26.814073693191403
32	32.95462972092678	21.645021997785147	20.650945387310145	24.74940289397793
33	32.41925113818879	21.502254375721684	20.569457018552455	25.50903746753707
34	29.100555832163295	22.447682430048346	24.655283828062803	23.796477909725557
35	27.505013571887815	25.517675234625386	24.273918262276833	22.703392931209965
36	27.953525553530117	26.079537537209625	21.116325461285285	24.850611447974973
37	28.749015009342642	25.82586424526695	20.701142222464878	24.72397852292553
38	28.736465800553958	26.553392401535568	21.644451579203842	23.06569021870663
39	30.174165090545802	23.559102291534415	21.300163221202624	24.96656939671716
40	28.43006953402506	24.177436033667735	20.819381845532277	26.573112586774926
41	25.73867169069623	25.27329161672109	22.012208587407276	26.97582810517541
42	25.022388929316175	22.665745304843913	21.51920395642328	30.792661809416632
43	25.840858105118365	21.02701420912686	24.411796582214837	28.720331103539937
44	25.6678582982458	21.35402703295145	23.439721831304407	29.53839283749834
45	27.664893751390395	21.776788690066322	22.69988893135338	27.858428627189895
46	26.788567833770248	23.888315301315465	21.41147633292562	27.911640531988667
47	25.115937576649998	23.48755550376517	23.69820293700379	27.698303982581045
48	26.987888383751546	22.039018260728554	25.552470768084916	25.420622587434984
49	27.824285000680426	22.39585582751846	23.12982156491893	26.650037606882183
50	25.208752828665	23.469791039375995	24.706051081798837	26.615405050160167
51	25.72701885396388	21.764157992908885	24.19935640486355	28.309466748263684
52	25.323081010032034	22.271830530269263	23.896382649822478	28.50870580987623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	34.0
4	68.0
5	53.5
6	39.0
7	28.0
8	17.0
9	182.5
10	348.0
11	1563.0
12	2778.0
13	3266.5
14	3230.5
15	2706.0
16	2416.5
17	2127.0
18	2304.5
19	2482.0
20	2419.5
21	2357.0
22	2675.5
23	2994.0
24	3020.0
25	3046.0
26	3629.5
27	4213.0
28	5098.5
29	5984.0
30	6835.5
31	7687.0
32	8250.0
33	8813.0
34	12991.5
35	17170.0
36	16903.5
37	16637.0
38	16613.5
39	19671.0
40	22752.0
41	26627.0
42	30502.0
43	35381.5
44	40261.0
45	43106.0
46	45951.0
47	48929.5
48	51908.0
49	57399.5
50	62891.0
51	68200.0
52	73509.0
53	75701.0
54	77893.0
55	89233.0
56	100573.0
57	108333.5
58	116094.0
59	111354.0
60	106614.0
61	108306.0
62	109998.0
63	103101.0
64	82169.5
65	68135.0
66	52578.5
67	37022.0
68	31847.0
69	26672.0
70	24467.5
71	22263.0
72	20896.5
73	19530.0
74	15421.5
75	11313.0
76	7657.0
77	4001.0
78	2975.0
79	1949.0
80	1533.0
81	1117.0
82	642.0
83	167.0
84	94.0
85	21.0
86	16.5
87	12.0
88	7.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1227169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.172932171526497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.79428337726074	4.791108641108111
2	9.915477597018993	1.422460965034156
3	4.411296919022085	0.9492580076582768
4	2.286876306461874	0.6561443452368827
5	1.30305371262383	0.46733579482532556
6	0.7770608015995637	0.33442826538154075
7	0.508952103971644	0.25554752442410134
8	0.3351358720348996	0.19231255026813748
9	0.24197946014723257	0.15621320290848287
>10	5.157684267926929	10.639039936634646
>50	4.777106243751704	24.901297213342254
>100	3.325229482868309	40.93584502216076
>500	0.11474143415432154	5.654070466252
>1k	0.04430609833681723	5.310678480307113
>5k	0.006816322821048806	3.3342595844582124
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9647	0.7861182934053909	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8183	0.6668193215441394	No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6300	0.5133767231734179	No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	5790	0.4718176551069983	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	5550	0.4522604466051538	No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5447	0.4438671446231122	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4044	0.3295389632560796	No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3883	0.3164193358860923	No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	3427	0.27926063973258775	No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3346	0.2726600818632153	No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2972	0.24218343194784092	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2531	0.20624706132570167	No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2308	0.18807515509273784	No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2182	0.1778076206292695	No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1847	0.1505090170954449	No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1752	0.14276762206346477	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1750	0.1426046453259494	No Hit
CTGAAAGGGGACTTGCACTGCTCCATCCGTCCAACATGTTCAAGTTCTTCGT	1623	0.13225562249372336	No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1555	0.12671441341820075	No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1529	0.12459571583050093	No Hit
CTGAAAGGGGATCACGCTCAAACATTCTCACAATCTAACCAACCAAACCAAC	1484	0.12092873923640508	No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1480	0.12060278576137434	No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1465	0.11938046023000908	No Hit
CTGAAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAA	1450	0.1181581346986438	No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1432	0.11669134406100547	No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1359	0.11074269314169441	No Hit
CTGAAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	1338	0.10903143739778302	No Hit
CTGAAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1254	0.10218641442213745	No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1230	0.100230693571953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.019638696870602174	0.0	0.0	0.0	0.0
7	0.019964650345632915	0.0	0.0	0.0	0.0
8	0.030069208071585904	0.0	0.0	0.0	0.0
9	0.06038288124944486	0.0	0.0	0.0	0.0
10	0.08621469414563113	0.0	0.0	0.0	0.0
11	0.12622548320565463	0.0	0.0	0.0	0.0
12	0.14415292433234542	0.0	0.0	0.0	0.0
13	0.1559687378022098	0.0	0.0	0.0	0.0
14	0.167051155953255	0.0	0.0	0.0	0.0
15	0.1766667834666619	0.0	0.0	0.0	0.0
16	0.18815664346149552	0.0	0.0	0.0	0.0
17	0.19858715466247925	0.0	0.0	0.0	0.0
18	0.2093436193384937	0.0	0.0	0.0	0.0
19	0.22881933947158054	0.0	0.0	0.0	0.0
20	0.2389238971975335	0.0	0.0	0.0	0.0
21	0.2502507804548518	0.0	0.0	0.0	0.0
22	0.2594589661244702	0.0	0.0	0.0	0.0
23	0.2690745936378771	0.0	0.0	0.0	0.0
24	0.27869022115128395	0.0	0.0	0.0	0.0
25	0.28814287192717547	0.0	0.0	0.0	0.0
26	0.29767701107182465	0.0	0.0	0.0	0.0
27	0.30501096426001634	0.0	0.0	0.0	0.0
28	0.3163378475173346	0.0	0.0	0.0	0.0
29	0.32505710297440693	0.0	0.0	0.0	0.0
30	0.3348357072253292	0.0	0.0	0.0	0.0
31	0.3424956138885516	0.0	0.0	0.0	0.0
32	0.35227421813947385	0.0	0.0	0.0	0.0
33	0.36156389217785	0.0	0.0	0.0	0.0
34	0.3737056591227451	0.0	0.0	0.0	0.0
35	0.3852770074863364	0.0	0.0	0.0	0.0
36	0.39782621627501996	0.0	0.0	0.0	0.0
37	0.4111088203825227	0.0	0.0	0.0	0.0
38	0.4217837966897795	0.0	0.0	0.0	8.148836875768537E-5
39	0.4316438893094594	0.0	0.0	0.0	8.148836875768537E-5
40	0.44199291214168546	0.0	0.0	0.0	8.148836875768537E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	35	1.0196527E-7	46.000004	43
CTCGATT	35	1.0196527E-7	46.000004	46
GTATACC	35	1.0196527E-7	46.000004	44
CGTATCT	30	1.8615083E-6	46.0	14
CTCGTAC	25	3.4172932E-5	46.0	44
CTACTAG	20	6.3116214E-4	46.0	26
TAACGCG	20	6.3116214E-4	46.0	20
AAACGAC	30	1.8615083E-6	46.0	14
CTACGTA	30	1.8615083E-6	46.0	46
CGTAGAT	25	3.4172932E-5	46.0	43
TTGCGTA	30	1.8615083E-6	46.0	13
TTGCGAT	20	6.3116214E-4	46.0	11
AACGAAT	30	1.8615083E-6	46.0	30
TCGTATA	20	6.3116214E-4	46.0	33
CGTACGT	30	1.8615083E-6	46.0	39
CGTTTAT	20	6.3116214E-4	46.0	40
TCGGTTA	20	6.3116214E-4	46.0	34
ACGAATT	25	3.4172932E-5	46.0	15
TAATCGG	30	1.8615083E-6	46.0	44
TACGCAT	45	3.092282E-10	45.999996	46
>>END_MODULE