FastQCFastQC Report
Fri 17 Jun 2016
SRR1527652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239253
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC92060.7428668722206039No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT78100.6302183654185223No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT59570.4806928044555874No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT55520.4480118264793388No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG53460.43138890928648144No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC51480.41541154227587096No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA39110.3155933453459463No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38400.3098640874785052No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33300.2687102633602662No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG32530.26249684285613994No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG27230.21972914328228377No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC24110.1945526861746552No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG22290.17986641952853857No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA21580.17413716166109747No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG17520.141375489912068No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT17260.1392774518197656No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16750.13516206940794173No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT14770.1191847023973313No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG14600.11781290826005665No Hit
CTGAAAGGGGACTTGCACTGCTCCATCCGTCCAACATGTTCAAGTTCTTCGT14510.11708666430502893No Hit
CTGAAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAA14240.11490793243994568No Hit
CTGAAAGGGGATCACGCTCAAACATTCTCACAATCTAACCAACCAAACCAAC13970.11272920057486245No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT13550.1093400621180663No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT13280.10716133025298305No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC13210.10659647384351702No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG13060.1053860672518041No Hit
CTGAAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG12850.10369149802340602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGAC453.110472E-1046.00000414
CGAACAG301.8615228E-646.021
CACGGGT301.8615228E-646.010
ACCGATA206.311646E-446.035
TAAACGT206.311646E-446.013
ACGGGTA253.4173114E-546.031
TAGTTAG206.311646E-446.020
GTTACGC253.4173114E-546.020
CTAACGG206.311646E-446.012
TATCTCG206.311646E-446.018
CGTAGAT253.4173114E-546.043
TTGCGTA253.4173114E-546.013
GTACTAT206.311646E-446.040
ACGTATT206.311646E-446.044
ATACGAG206.311646E-446.040
CGATCAT405.6115823E-946.034
TATCGGG253.4173114E-546.046
ACGGTAC206.311646E-446.029
TACGCAT351.0196527E-745.99999646
TGTTACG351.0196527E-745.99999629
Produced by FastQC (version 0.11.5)