##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239253
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14275131873798	33.0	31.0	34.0	31.0	34.0
2	32.550516319105135	34.0	31.0	34.0	31.0	34.0
3	32.87075197719917	34.0	31.0	34.0	31.0	34.0
4	36.02594788957541	37.0	35.0	37.0	35.0	37.0
5	36.27685468584704	37.0	37.0	37.0	35.0	37.0
6	36.31874766492395	37.0	37.0	37.0	35.0	37.0
7	36.3499196693492	37.0	36.0	37.0	35.0	37.0
8	36.52770015485135	37.0	37.0	37.0	35.0	37.0
9	38.64718665195888	39.0	39.0	39.0	38.0	39.0
10	38.011259202116115	39.0	38.0	39.0	37.0	39.0
11	37.73712631722498	39.0	38.0	39.0	35.0	39.0
12	37.117415894897974	39.0	35.0	39.0	34.0	39.0
13	36.83024128244999	39.0	35.0	39.0	33.0	39.0
14	37.72155483989145	40.0	37.0	40.0	33.0	40.0
15	37.806477773303754	40.0	37.0	40.0	33.0	40.0
16	37.937213789274665	40.0	37.0	40.0	34.0	40.0
17	37.846557563306284	40.0	37.0	40.0	33.0	40.0
18	37.84192654768639	40.0	37.0	40.0	33.0	40.0
19	37.798100952751376	40.0	37.0	40.0	34.0	40.0
20	37.77825431933592	40.0	37.0	40.0	33.0	40.0
21	37.78317018397373	40.0	37.0	40.0	33.0	40.0
22	37.70691053400718	40.0	37.0	40.0	33.0	40.0
23	37.69617342060096	40.0	36.0	40.0	34.0	40.0
24	37.69356539786468	40.0	36.0	40.0	34.0	40.0
25	37.66139601840786	40.0	36.0	40.0	34.0	40.0
26	37.49579141628062	40.0	36.0	40.0	33.0	40.0
27	37.38945396944772	40.0	36.0	40.0	33.0	40.0
28	37.34630216751543	40.0	35.0	40.0	33.0	40.0
29	37.26652830374427	40.0	35.0	40.0	33.0	40.0
30	37.078651413391775	40.0	35.0	40.0	32.0	40.0
31	37.03880321451713	39.0	35.0	40.0	32.0	40.0
32	36.93158217087229	39.0	35.0	40.0	32.0	40.0
33	36.67652125917791	39.0	35.0	40.0	31.0	40.0
34	36.508369154643965	39.0	35.0	40.0	30.0	40.0
35	36.516441759672965	39.0	35.0	40.0	31.0	40.0
36	36.40785941208131	39.0	35.0	40.0	31.0	40.0
37	36.447592218860876	39.0	35.0	40.0	31.0	40.0
38	36.071905413987295	38.0	35.0	40.0	30.0	40.0
39	36.13695347116368	38.0	35.0	40.0	30.0	40.0
40	36.032392094269696	38.0	35.0	40.0	30.0	40.0
41	35.669487989942326	38.0	35.0	40.0	29.0	40.0
42	35.7712807634922	38.0	34.0	40.0	30.0	40.0
43	35.79527505682859	38.0	35.0	40.0	30.0	40.0
44	35.59442018699975	37.0	34.0	40.0	30.0	40.0
45	35.45827042581297	37.0	34.0	40.0	29.0	40.0
46	35.15520236787807	37.0	34.0	40.0	28.0	40.0
47	34.90556569159001	36.0	34.0	40.0	27.0	40.0
48	34.863362041487896	36.0	33.0	40.0	28.0	40.0
49	34.798974866310594	36.0	33.0	40.0	28.0	40.0
50	34.6166868266609	35.0	33.0	39.0	27.0	40.0
51	34.92029876062434	36.0	34.0	40.0	28.0	40.0
52	34.64728671223713	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	13.0
16	31.0
17	100.0
18	180.0
19	312.0
20	613.0
21	1017.0
22	1721.0
23	2572.0
24	3720.0
25	5399.0
26	7380.0
27	9939.0
28	12547.0
29	16045.0
30	21071.0
31	27230.0
32	36835.0
33	53967.0
34	100031.0
35	100375.0
36	124619.0
37	198453.0
38	363719.0
39	151362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.74097298937343	0.18172237630249838	0.05035291421525709	0.026951720108807482
8	99.7714752354846	0.1549320437392526	0.05906784167559005	0.014524879100554932
9	99.60274455659982	0.1876130217155012	0.12547881667423844	0.08416360501043774
10	68.55993086157548	22.52832956627904	2.4875469335155933	6.424192638629885
11	39.40583561225996	30.295992827937475	14.865406821690163	15.432764738112395
12	33.108251503123256	16.709824386142298	24.044726944376976	26.137197166357474
13	25.674095604368112	18.001408913272755	26.785168161787787	29.539327320571346
14	22.914126493944337	20.04110540785457	29.59823377470137	27.446534323499723
15	23.576138205838518	21.21875032781845	27.92448353968076	27.28062792666227
16	29.74832419207377	20.830855362060856	24.077972778762692	25.34284766710268
17	30.323267323137404	20.303440863165147	23.697017477464247	25.676274336233202
18	28.281150015372162	21.284999915271538	23.844041531470975	26.58980853788532
19	29.17241273573677	22.4344827085349	21.246186210563945	27.146918345164384
20	29.843381456409627	22.401721036785872	22.50226547767082	25.252632029133682
21	28.976488255424844	23.053807414628004	23.693789726553014	24.275914603394142
22	29.519718733785595	20.392284706996875	24.530422762744976	25.557573796472553
23	28.27445243223135	23.216647448099785	23.172346566843093	25.336553552825773
24	28.646934887387804	21.171141001877743	23.11247178743969	27.069452323294758
25	28.351757066555415	23.412975397275616	21.282094939451426	26.95317259671754
26	26.250813998432925	22.938334625778594	23.1339363309994	27.67691504478908
27	25.01749037525025	20.981833410933845	23.157821687742537	30.84285452607337
28	25.987510217848982	22.33337341124048	21.444128035195394	30.234988335715148
29	29.006506338899317	22.47135976269575	21.29177819218513	27.230355706219793
30	26.27542559913109	21.6561912700635	23.6082543274053	28.460128803400114
31	29.688126637579256	21.48883238531599	22.039405996999804	26.78363498010495
32	32.95816108575085	21.640052515507328	20.714333554165293	24.687452844576534
33	32.375229271181915	21.564442450411658	20.586353230534847	25.473975047871583
34	29.088733293363017	22.509850692312224	24.684789950074762	23.716626064249997
35	27.53868661201546	25.47954291819346	24.240570730916126	22.74119973887495
36	28.05674063326859	25.990980050078555	21.19865757839602	24.753621738256836
37	28.662750866852853	25.784565379305114	20.664787577677842	24.88789617616419
38	28.762649757555558	26.498382493324605	21.59268527088496	23.146282478234873
39	30.20158111378387	23.560725695237373	21.279996901359123	24.957696289619633
40	28.34449462700514	24.20466200202864	20.871525023542407	26.579318347423815
41	25.935301346859763	25.230683322937285	22.076928601342903	26.757086728860045
42	24.994250568689363	22.573114610172418	21.591071395429342	30.841563425708873
43	25.843633220980706	21.004508361085268	24.522837548103578	28.629020869830452
44	25.6370571626617	21.27830233213073	23.462521373763064	29.622119131444506
45	27.584520674954994	21.75697779226679	22.663733717005325	27.994767815772885
46	26.875141718438446	23.87163880176203	21.41152775099193	27.841691728807593
47	25.374076157168872	23.42911415183179	23.639724898789837	27.5570847922095
48	26.91153460996261	22.160123881079976	25.54990788805837	25.378433620899045
49	27.80812311933076	22.320785182686667	23.14426513391535	26.726826564067224
50	25.294027934570263	23.43661867270041	24.689873657759957	26.579479734969375
51	25.784565379305114	21.775537360006393	24.14357681603353	28.296320444654967
52	25.420152301426747	22.30077312703701	23.81813883040832	28.460935741127923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	23.5
4	47.0
5	39.0
6	31.0
7	22.5
8	14.0
9	160.0
10	306.0
11	1484.5
12	2663.0
13	3208.5
14	3311.5
15	2869.0
16	2580.0
17	2291.0
18	2374.0
19	2457.0
20	2398.5
21	2340.0
22	2682.5
23	3025.0
24	3074.0
25	3123.0
26	3632.0
27	4141.0
28	4937.0
29	5733.0
30	6757.5
31	7782.0
32	8401.0
33	9020.0
34	13164.5
35	17309.0
36	17032.0
37	16755.0
38	16844.0
39	19816.0
40	22699.0
41	26963.0
42	31227.0
43	35968.5
44	40710.0
45	43334.5
46	45959.0
47	49430.0
48	52901.0
49	58088.5
50	63276.0
51	68961.0
52	74646.0
53	77103.0
54	79560.0
55	90997.5
56	102435.0
57	109354.0
58	116273.0
59	111954.0
60	107635.0
61	109343.0
62	111051.0
63	103517.5
64	82135.5
65	68287.0
66	53047.0
67	37807.0
68	32403.0
69	26999.0
70	24761.0
71	22523.0
72	21100.0
73	19677.0
74	15607.5
75	11538.0
76	7885.0
77	4232.0
78	3061.5
79	1891.0
80	1519.5
81	1148.0
82	658.5
83	169.0
84	96.0
85	23.0
86	12.5
87	2.0
88	3.5
89	3.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1239253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.803818512098825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04043189845379	7.891155702762085
2	10.11573043849163	2.185770315495549
3	3.771025991297926	1.2222444124317107
4	1.8292883923359007	0.7905319918834441
5	0.9898867596904842	0.5347278449612787
6	0.5533010340075895	0.358665837238477
7	0.352832636509073	0.2668357838992551
8	0.2815911124710313	0.24338074190056241
9	0.19020706923446695	0.1849466390154657
>10	3.6182761714510403	11.235072856549435
>50	3.142775587231367	24.6524318852999
>100	2.0128141001853503	37.2393499749689
>500	0.07038858832493412	5.225288122207352
>1k	0.026957331698910937	4.812937821708956
>5k	0.004492888616485156	3.156660069677634
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9206	0.7428668722206039	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7810	0.6302183654185223	No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5957	0.4806928044555874	No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	5552	0.4480118264793388	No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5346	0.43138890928648144	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	5148	0.41541154227587096	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3911	0.3155933453459463	No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3840	0.3098640874785052	No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3330	0.2687102633602662	No Hit
CTGAAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	3253	0.26249684285613994	No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2723	0.21972914328228377	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2411	0.1945526861746552	No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2229	0.17986641952853857	No Hit
CTGAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2158	0.17413716166109747	No Hit
CTGAAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1752	0.141375489912068	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1726	0.1392774518197656	No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1675	0.13516206940794173	No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1477	0.1191847023973313	No Hit
CTGAAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1460	0.11781290826005665	No Hit
CTGAAAGGGGACTTGCACTGCTCCATCCGTCCAACATGTTCAAGTTCTTCGT	1451	0.11708666430502893	No Hit
CTGAAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAA	1424	0.11490793243994568	No Hit
CTGAAAGGGGATCACGCTCAAACATTCTCACAATCTAACCAACCAAACCAAC	1397	0.11272920057486245	No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1355	0.1093400621180663	No Hit
CTGAAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1328	0.10716133025298305	No Hit
CTGAAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1321	0.10659647384351702	No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1306	0.1053860672518041	No Hit
CTGAAAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	1285	0.10369149802340602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.018398180194036245	0.0	0.0	0.0	0.0
7	0.01872095528515969	0.0	0.0	0.0	0.0
8	0.028081432927739534	0.0	0.0	0.0	0.0
9	0.057292578674411114	0.0	0.0	0.0	0.0
10	0.0838408299193143	0.0	0.0	0.0	0.0
11	0.12265453462690831	0.0	0.0	0.0	0.0
12	0.13935814559254647	0.0	0.0	0.0	0.0
13	0.1520270679191416	0.0	0.0	0.0	0.0
14	0.161064770470598	0.0	0.0	0.0	0.0
15	0.17058663565873958	0.0	0.0	0.0	0.0
16	0.18067335725634717	0.0	0.0	0.0	0.0
17	0.19237395430957197	0.0	0.0	0.0	0.0
18	0.20318691986220733	0.0	0.0	0.0	0.0
19	0.21868012423613256	0.0	0.0	0.0	0.0
20	0.2286861520609593	0.0	0.0	0.0	0.0
21	0.23925703629525205	0.0	0.0	0.0	0.0
22	0.24877890148339363	0.0	0.0	0.0	0.0
23	0.25894631685378205	0.0	0.0	0.0	0.0
24	0.26822610072358105	0.0	0.0	0.0	0.0
25	0.27790935345728435	0.0	0.0	0.0	0.0
26	0.2876732999637685	0.0	0.0	0.0	0.0
27	0.2963882274241015	0.0	0.0	0.0	0.0
28	0.3074432742950794	0.0	0.0	0.0	0.0
29	0.31736860834712527	0.0	0.0	0.0	0.0
30	0.32672908598970507	0.0	0.0	0.0	0.0
31	0.33439499440388687	0.0	0.0	0.0	0.0
32	0.34730599804882456	0.0	0.0	0.0	0.0
33	0.35577884419081496	0.0	0.0	0.0	0.0
34	0.3669952786073546	0.0	0.0	0.0	0.0
35	0.37893795697892196	0.0	0.0	0.0	0.0
36	0.39007369762268074	0.0	0.0	0.0	0.0
37	0.4012901320392204	0.0	0.0	0.0	0.0
38	0.41056991590901937	0.0	0.0	0.0	0.0
39	0.4225125942805868	0.0	0.0	0.0	0.0
40	0.433406253606003	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGAC	45	3.110472E-10	46.000004	14
CGAACAG	30	1.8615228E-6	46.0	21
CACGGGT	30	1.8615228E-6	46.0	10
ACCGATA	20	6.311646E-4	46.0	35
TAAACGT	20	6.311646E-4	46.0	13
ACGGGTA	25	3.4173114E-5	46.0	31
TAGTTAG	20	6.311646E-4	46.0	20
GTTACGC	25	3.4173114E-5	46.0	20
CTAACGG	20	6.311646E-4	46.0	12
TATCTCG	20	6.311646E-4	46.0	18
CGTAGAT	25	3.4173114E-5	46.0	43
TTGCGTA	25	3.4173114E-5	46.0	13
GTACTAT	20	6.311646E-4	46.0	40
ACGTATT	20	6.311646E-4	46.0	44
ATACGAG	20	6.311646E-4	46.0	40
CGATCAT	40	5.6115823E-9	46.0	34
TATCGGG	25	3.4173114E-5	46.0	46
ACGGTAC	20	6.311646E-4	46.0	29
TACGCAT	35	1.0196527E-7	45.999996	46
TGTTACG	35	1.0196527E-7	45.999996	29
>>END_MODULE