FastQCFastQC Report
Fri 17 Jun 2016
SRR1527651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674169
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC31250.4635336243582841No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC26840.39811975928884297No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC25050.37156855328560046No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC24010.35614215426695683No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT19320.28657502792326556No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG15830.23480759275493235No Hit
GTATACGGGGGGGCTGGAGAGATGGCTTAGAGGTTAAGAGCACTGACTGC14440.2141896171434759No Hit
GTATACGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTG14250.21137133270737754No Hit
GTATACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA14090.20899804055066312No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG11070.16420215109267855No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA10920.16197718969575878No Hit
GTATACGGGAGCTTTTTGATAACCACTGGAAAGAATTTTATTGAAAGTTC10080.1495174058730081No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC10080.1495174058730081No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG9780.14506748307916859No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG9720.14417749852040068No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT9130.13542598369251627No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8040.11925793087489932No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG7940.11777462327695282No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG7250.1075398008511219No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC7150.10605649325317539No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC6790.10071658590056795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAT207.8541617E-444.0000048
ACACCGA207.8541617E-444.00000441
ATAATCG207.8541617E-444.00000412
ACGATTC207.8541617E-444.00000431
GCCGATA207.8541617E-444.00000436
TTGACCG207.8541617E-444.00000429
GCGATAA207.8541617E-444.0000049
CATAAGG207.8541617E-444.00000431
TATAGCG207.8541617E-444.00000442
TTACGCG207.8541617E-444.00000417
CGATCGT207.8541617E-444.00000437
AGTACGT207.8541617E-444.00000432
GCGTAAG207.8541617E-444.00000419
GTACGTC207.8541617E-444.00000433
GTACGTA207.8541617E-444.0000049
TACCGTA207.8541617E-444.00000442
TACGTAG207.8541617E-444.00000410
TACGTAC408.303687E-944.00000425
TAATCGA207.8541617E-444.00000413
AACCGTT254.4408764E-544.024
Produced by FastQC (version 0.11.5)