##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527651_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	674169
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41491525122039	33.0	31.0	34.0	31.0	34.0
2	31.637079130010427	31.0	31.0	33.0	30.0	34.0
3	32.336660392275526	33.0	31.0	34.0	31.0	34.0
4	36.454469131627235	37.0	37.0	37.0	35.0	37.0
5	36.46083400453002	37.0	37.0	37.0	35.0	37.0
6	36.45519150242743	37.0	37.0	37.0	35.0	37.0
7	36.54291283046239	37.0	37.0	37.0	35.0	37.0
8	36.682041149919385	37.0	37.0	37.0	35.0	37.0
9	38.753495043527664	39.0	39.0	39.0	39.0	39.0
10	38.297306758394406	39.0	39.0	39.0	37.0	39.0
11	38.03484882870615	39.0	38.0	39.0	37.0	39.0
12	37.572359452896826	39.0	37.0	39.0	35.0	39.0
13	37.46559690522703	39.0	37.0	39.0	35.0	39.0
14	38.24207579998487	40.0	38.0	40.0	35.0	40.0
15	38.26594963577382	40.0	38.0	40.0	35.0	40.0
16	38.32595387803355	40.0	38.0	40.0	35.0	40.0
17	38.28165193000568	40.0	38.0	40.0	35.0	40.0
18	38.195596356403215	40.0	38.0	40.0	34.0	40.0
19	38.17875488193613	40.0	38.0	40.0	34.0	40.0
20	38.053689801815274	40.0	38.0	40.0	34.0	40.0
21	37.99580372280541	40.0	38.0	40.0	34.0	40.0
22	38.02913957776166	40.0	38.0	40.0	34.0	40.0
23	37.93200517971013	40.0	38.0	40.0	34.0	40.0
24	37.83007524819445	40.0	37.0	40.0	34.0	40.0
25	37.80458164050854	40.0	37.0	40.0	34.0	40.0
26	37.694538016432084	40.0	37.0	40.0	34.0	40.0
27	37.556151350774066	40.0	36.0	40.0	33.0	40.0
28	37.366645455368015	39.0	36.0	40.0	33.0	40.0
29	37.45597172222395	40.0	37.0	40.0	33.0	40.0
30	37.64089716376754	40.0	37.0	40.0	34.0	40.0
31	37.65697473482168	40.0	37.0	40.0	34.0	40.0
32	37.63607493076662	40.0	36.0	40.0	34.0	40.0
33	37.5171952433292	40.0	36.0	40.0	33.0	40.0
34	37.14657897352148	40.0	35.0	40.0	33.0	40.0
35	37.13725193534559	40.0	35.0	40.0	33.0	40.0
36	37.0420384206334	40.0	35.0	40.0	33.0	40.0
37	36.90791033108909	39.0	35.0	40.0	32.0	40.0
38	36.75301148525073	39.0	35.0	40.0	32.0	40.0
39	36.609164764324674	39.0	35.0	40.0	32.0	40.0
40	36.38582313930187	39.0	35.0	40.0	31.0	40.0
41	36.22792504550046	39.0	35.0	40.0	31.0	40.0
42	36.13143588625404	39.0	35.0	40.0	31.0	40.0
43	36.03725772024522	38.0	35.0	40.0	31.0	40.0
44	35.74201869264235	38.0	35.0	40.0	30.0	40.0
45	35.66997444261009	37.0	35.0	40.0	30.0	40.0
46	35.527338100683956	37.0	35.0	40.0	30.0	40.0
47	35.421023511908736	37.0	35.0	40.0	30.0	40.0
48	35.28503980455939	37.0	35.0	40.0	29.0	40.0
49	35.18256253254006	36.0	34.0	40.0	29.0	40.0
50	34.65273544170675	35.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	6.0
16	21.0
17	53.0
18	99.0
19	169.0
20	266.0
21	418.0
22	669.0
23	986.0
24	1537.0
25	2336.0
26	3157.0
27	4266.0
28	5887.0
29	7836.0
30	9952.0
31	12280.0
32	14902.0
33	20270.0
34	40842.0
35	48141.0
36	67541.0
37	110929.0
38	226550.0
39	95052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.47401912576817	0.447810563820051	0.05384406580545828	0.02432624460632275
8	99.39199221560172	0.5055112293801702	0.03990097438476109	0.06259558063334268
9	97.20070190115536	1.6521080025928219	0.5443738884463688	0.6028162078054612
10	55.36267612423591	33.226831847800774	5.02247952664688	6.388012501316435
11	36.154287723108006	23.812426854394076	22.0647938424935	17.968491580004418
12	29.354064040322235	17.752077001464027	26.81968467847083	26.074174279742913
13	25.37939300086477	17.67094007585635	29.36949043934088	27.580176483938
14	25.703940703295462	18.856547838894997	29.4742119557559	25.96529950205364
15	26.528659727753723	19.834640868980923	27.54220380943057	26.094495593834782
16	29.889538083180923	20.095258013940125	24.94819548214172	25.067008420737235
17	31.233859759199845	20.530757124697217	22.93490207944892	25.300481036654016
18	29.19475680430278	21.276712515704517	23.51279871960888	26.01573196038382
19	29.221456341065817	21.688033712615088	21.909046544709117	27.181463401609985
20	28.20094071367862	22.58780810152944	23.64199481139002	25.56925637340192
21	30.3339370395257	20.984204257389468	24.94211392099014	23.739744782094697
22	30.433911971627293	19.990684828284895	24.46256057457403	25.11284262551378
23	28.02591041712093	22.32659763353106	23.996208665779648	25.651283283568365
24	27.705960968243872	22.764766697964454	22.861775014870158	26.66749731892152
25	29.05473256705663	23.041848557260867	22.795026172962565	25.10839270271994
26	27.12109278237356	22.35433548561266	23.084271154562135	27.440300577451648
27	26.794468449305736	21.257429516931214	23.612476990190885	28.33562504357216
28	28.58823232750245	22.55146706537975	23.147460058234657	25.712840548883143
29	29.169837236657276	22.930897148934466	21.596365303061994	26.302900311346267
30	28.602620411202533	20.932140160701547	22.618957561086315	27.84628186700961
31	29.38165356164404	22.098761586486475	22.313247865149542	26.206336986719947
32	32.53397886879996	21.9261045820855	21.711914964942025	23.828001584172515
33	31.622634680621626	21.92699456664427	22.358637077646705	24.091733675087404
34	29.27900867586614	23.642143142149816	24.543994161701292	22.53485402028275
35	28.868132471234958	24.7212494196559	24.864240272097945	21.546377837011192
36	29.625805992266034	24.04916274702634	21.611346709801253	24.713684550906375
37	29.43698093504744	24.970148434591326	21.61194003284043	23.9809305975208
38	30.321180594183357	23.896382064437848	22.288328297504037	23.494109043874754
39	29.1081316405827	23.351859845231683	21.86543730132949	25.674571212856122
40	28.65201455421415	23.909286840539984	22.123236161852592	25.315462443393272
41	27.384676542528652	23.43299677083936	23.44441823934355	25.737908447288437
42	25.445400188973387	22.974803053833686	24.15729587091664	27.42250088627629
43	27.298644701847756	21.149593054560505	24.86527858741651	26.68648365617523
44	26.77770707344894	21.604820156370288	24.9875031334873	26.629969636693467
45	28.156886478019604	21.377132440085497	23.92322993196068	26.54275114993421
46	27.73889039691828	22.94854850935003	23.062021540592937	26.25053955313875
47	26.852762437905035	23.64955968013955	23.367137913490534	26.130539968464884
48	28.285934239040955	22.7690682899985	24.627504379465684	24.317493091494864
49	27.84806183612714	23.02953710419791	24.244514357675897	24.877886701999053
50	25.92584351994826	22.89500110506416	23.410895487629958	27.768259887357623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	5.0
6	6.0
7	20.5
8	35.0
9	306.0
10	577.0
11	1154.5
12	1732.0
13	1676.5
14	1621.0
15	1645.0
16	1669.0
17	1705.0
18	1741.0
19	1724.5
20	1708.0
21	1939.0
22	2170.0
23	2414.0
24	2658.0
25	3259.0
26	3860.0
27	4686.5
28	5513.0
29	5187.0
30	4861.0
31	5522.5
32	6184.0
33	8200.5
34	10217.0
35	9808.0
36	9399.0
37	10503.5
38	11608.0
39	13060.0
40	14512.0
41	15454.5
42	16397.0
43	18893.5
44	21390.0
45	22749.5
46	24109.0
47	26495.5
48	28882.0
49	31461.5
50	34041.0
51	37826.0
52	41611.0
53	43836.5
54	46062.0
55	53550.0
56	61038.0
57	63655.0
58	66272.0
59	63228.0
60	60184.0
61	59116.0
62	58048.0
63	49905.5
64	41763.0
65	36908.5
66	32054.0
67	25800.0
68	19546.0
69	16706.5
70	13867.0
71	12238.5
72	10610.0
73	10954.0
74	11298.0
75	7781.5
76	4265.0
77	2953.5
78	1642.0
79	1166.0
80	690.0
81	479.5
82	269.0
83	150.5
84	32.0
85	23.0
86	14.0
87	10.0
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	674169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.416972598858742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.84718345958876	7.171792236071371
2	8.527937574756507	1.7767058408203287
3	3.280742723699949	1.0252622117006271
4	1.610468758899584	0.6710483573110007
5	0.8985020219855329	0.46798354715212354
6	0.568149456057413	0.35510383894839426
7	0.42433217520077465	0.30941796493164175
8	0.3645269692999943	0.30378139605944504
9	0.32608076550663556	0.3057096959367755
>10	9.71122629150766	29.586943333199834
>50	3.994133394087828	28.37226274124144
>100	1.3840633365609158	23.341476692046058
>500	0.04414193768867119	2.941992289767106
>1k	0.018511135159765334	3.3705198548138524
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3125	0.4635336243582841	No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2684	0.39811975928884297	No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2505	0.37156855328560046	No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2401	0.35614215426695683	No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1932	0.28657502792326556	No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1583	0.23480759275493235	No Hit
GTATACGGGGGGGCTGGAGAGATGGCTTAGAGGTTAAGAGCACTGACTGC	1444	0.2141896171434759	No Hit
GTATACGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTG	1425	0.21137133270737754	No Hit
GTATACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	1409	0.20899804055066312	No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1107	0.16420215109267855	No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1092	0.16197718969575878	No Hit
GTATACGGGAGCTTTTTGATAACCACTGGAAAGAATTTTATTGAAAGTTC	1008	0.1495174058730081	No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1008	0.1495174058730081	No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	978	0.14506748307916859	No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	972	0.14417749852040068	No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	913	0.13542598369251627	No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	804	0.11925793087489932	No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	794	0.11777462327695282	No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	725	0.1075398008511219	No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	715	0.10605649325317539	No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	679	0.10071658590056795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.030704467277492736	0.0	0.0	0.0	0.0
8	0.03485772855174296	0.0	0.0	0.0	0.0
9	0.03826933602701993	0.0	0.0	0.0	0.0
10	0.054289058084842225	0.0	0.0	0.0	0.0
11	0.0667488419075929	0.0	0.0	0.0	0.0
12	0.07475870293650405	0.0	0.0	0.0	0.0
13	0.08425187156336171	0.0	0.0	0.0	0.0
14	0.09285505563145147	0.0	0.0	0.0	0.0
15	0.10709480857173795	0.0	0.0	0.0	0.0
16	0.11999958467387258	0.0	0.0	0.0	0.0
17	0.134684329893543	0.0	0.0	0.0	0.0
18	0.14669912143690975	0.0	0.0	0.0	0.0
19	0.15915890525966042	0.0	0.0	0.0	0.0
20	0.17473363503809874	0.0	0.0	0.0	0.0
21	0.18615510354228687	0.0	0.0	0.0	0.0
22	0.20158150256093058	0.0	0.0	0.0	0.0
23	0.21789788613834216	0.0	0.0	0.0	0.0
24	0.2343626004755484	0.0	0.0	0.0	0.0
25	0.24845402265604025	0.0	0.0	0.0	0.0
26	0.2681820137087288	0.0	0.0	0.0	0.0
27	0.28331175120778324	0.0	0.0	0.0	0.0
28	0.304226388338829	0.0	0.0	0.0	0.0
29	0.3208394334358299	0.0	0.0	0.0	0.0
30	0.3378974708122147	0.0	0.0	0.0	0.0
31	0.3491706085566082	0.0	0.0	0.0	0.0
32	0.3654869921340198	0.0	0.0	0.0	0.0
33	0.37735345291759187	0.0	0.0	0.0	0.0
34	0.3890715829413693	0.0	0.0	0.0	0.0
35	0.407464597155906	0.0	0.0	0.0	0.0
36	0.423187657694139	0.0	0.0	0.0	0.0
37	0.43787240291380947	0.0	0.0	0.0	0.0
38	0.4488488791386136	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAT	20	7.8541617E-4	44.000004	8
ACACCGA	20	7.8541617E-4	44.000004	41
ATAATCG	20	7.8541617E-4	44.000004	12
ACGATTC	20	7.8541617E-4	44.000004	31
GCCGATA	20	7.8541617E-4	44.000004	36
TTGACCG	20	7.8541617E-4	44.000004	29
GCGATAA	20	7.8541617E-4	44.000004	9
CATAAGG	20	7.8541617E-4	44.000004	31
TATAGCG	20	7.8541617E-4	44.000004	42
TTACGCG	20	7.8541617E-4	44.000004	17
CGATCGT	20	7.8541617E-4	44.000004	37
AGTACGT	20	7.8541617E-4	44.000004	32
GCGTAAG	20	7.8541617E-4	44.000004	19
GTACGTC	20	7.8541617E-4	44.000004	33
GTACGTA	20	7.8541617E-4	44.000004	9
TACCGTA	20	7.8541617E-4	44.000004	42
TACGTAG	20	7.8541617E-4	44.000004	10
TACGTAC	40	8.303687E-9	44.000004	25
TAATCGA	20	7.8541617E-4	44.000004	13
AACCGTT	25	4.4408764E-5	44.0	24
>>END_MODULE