FastQCFastQC Report
Fri 17 Jun 2016
SRR1527650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018217
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA45000.44194901479743515No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT39360.3865580716094899No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC35020.3439345444045817No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC34100.33489914232427864No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29100.2857936962356747No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23210.22794748074329935No Hit
GTATACGGGGGGGCTGGAGAGATGGCTTAGAGGTTAAGAGCACTGACTGCTC22740.22333156881097055No Hit
GTATACGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGT20880.2050643428660099No Hit
GTATACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG20640.20270728145375688No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17650.17334222469277177No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG16020.1573338492678869No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15530.15252151555120372No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15050.14780739272669774No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14730.1446646441770271No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC13790.13543282031236958No Hit
GTATACGGGAGCTTTTTGATAACCACTGGAAAGAATTTTATTGAAAGTTCTG13140.12904911232085106No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG12100.11883517953442144No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG11340.11137115172895366No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10890.10695166158097931No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10650.1045946001687263No Hit
GTATACGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC10530.1034160694625998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA206.311112E-446.00000445
AACCGTT351.0194162E-746.00000424
ATTAGCG206.311112E-446.00000443
GATAAAC206.311112E-446.00000423
CGAGTAT351.0194162E-746.00000443
TTTACGT351.0194162E-746.00000418
CGACGGA206.311112E-446.00000428
TAGCGTC405.6097633E-946.00000426
ATACTAG206.311112E-446.00000410
CGGATTA206.311112E-446.0000046
CCATACT206.311112E-446.00000422
CGTACAT206.311112E-446.00000434
CGTAACA206.311112E-446.00000429
CGCAAAT351.0194162E-746.00000433
TCGACGT206.311112E-446.00000435
CGATAGT206.311112E-446.00000413
TCGAATA206.311112E-446.00000443
ACGCGAA301.8611918E-646.033
AACGTAA1100.046.037
CTACGTT301.8611918E-646.028
Produced by FastQC (version 0.11.5)