FastQCFastQC Report
Fri 17 Jun 2016
SRR1527649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024332
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA43590.42554562387975775No Hit
GTATACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT38200.37292596540965234No Hit
GTATACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC33500.3270424042205066No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC31950.31191059148791606No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG28120.274520370348676No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT22410.2187767247337777No Hit
GTATACGGGGGGGCTGGAGAGATGGCTTAGAGGTTAAGAGCACTGACTGCTC21020.20520690557358356No Hit
GTATACGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGT20420.19934942967709687No Hit
GTATACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG19570.19105133882374076No Hit
GTATACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG16190.15805422460686575No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16190.15805422460686575No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15800.1542468652741494No Hit
GTATACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14160.13823643115708578No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14010.13677206218296412No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13950.13618631459331546No Hit
GTATACGGGAGCTTTTTGATAACCACTGGAAAGAATTTTATTGAAAGTTCTG13000.12691197775721155No Hit
GTATACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG12240.11949250828832839No Hit
GTATACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10870.10611793832468379No Hit
GTATACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG10360.10113908381267012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAT700.046.0000045
CGATACT351.0194162E-746.00000426
AACGTAA1000.046.037
GGTACGC405.6097633E-946.08
TTTACGT405.6097633E-946.018
CGCATAT253.4168945E-546.032
CGTTGAC206.311132E-446.017
CGCGATT206.311132E-446.044
ATATGCG206.311132E-446.013
TATGCGA206.311132E-446.014
CGTTCAT253.4168945E-546.017
CCCCGTA206.311132E-446.023
TCGCTTA206.311132E-446.014
GAACGTA206.311132E-446.037
ACGCTAA206.311132E-446.029
TCGTAGT206.311132E-446.018
TCGTAAT206.311132E-446.016
CGTAATC206.311132E-446.017
CGTAACC253.4168945E-546.015
ACGTAAT1000.046.038
Produced by FastQC (version 0.11.5)