FastQCFastQC Report
Fri 17 Jun 2016
SRR1527648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038428
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC64490.6210348719410494No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT59160.5697072883242749No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37220.358426390659728No Hit
GAATTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA23190.22331832346585415No Hit
GAATTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20170.19423590272989558No Hit
GAATTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG19680.1895172318157831No Hit
GAATTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT18680.1798872911747372No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG18340.1766131113567816No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG17490.16842766181189261No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT16700.16082000870546634No Hit
GAATTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG15160.14598990011825566No Hit
GAATTAGGGGTGCAGTGCTGAATTGGGGGCCAACCAGTAGAACACCCATT14670.14127122920414317No Hit
GAATTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG14230.137034055322083No Hit
GAATTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA14140.13616736066438886No Hit
GAATTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG14060.1353969654131052No Hit
GAATTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC13840.1332783784720751No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC13490.129907899247709No Hit
GAATTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT13270.12778931230667895No Hit
GAATTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12350.11892976691691673No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA12300.11844826988486443No Hit
GAATTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC12200.11748527582075984No Hit
GAATTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA11870.1143073954092147No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11860.11421109600280423No Hit
GAATTAGGGACTTGGGTGTATATTGTTAATTATTCTTTTGGATTCTTTTG11800.11363329956434148No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG11730.11295920371946827No Hit
GAATTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC11410.10987762271433359No Hit
GAATTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT11370.10949242508869175No Hit
GAATTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT11340.10920352686946037No Hit
GAATTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA10650.10255886782713872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA254.4425426E-544.043
TAATACA254.4425426E-544.036
TCCGATT207.8561314E-444.038
ACGTTAC254.4425426E-544.018
ATAGCGA254.4425426E-544.021
ATTCGTA207.8561314E-444.033
TACGGTT207.8561314E-444.040
TAAGTCG207.8561314E-444.035
CGATTAT207.8561314E-444.024
CGTTCGA207.8561314E-444.014
TTATCGC207.8561314E-444.040
AAAACGT351.4458965E-744.028
ATCGTTT207.8561314E-444.035
TTGCGAA207.8561314E-444.044
CGTACTT207.8561314E-444.040
TCGTAGT302.5274803E-644.043
ACGTAAC502.7284841E-1144.011
GATCTAT502.7284841E-1144.09
ATACGGT254.4425426E-544.010
TAGTGCC207.8561314E-444.030
Produced by FastQC (version 0.11.5)