##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038428
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28769255066312	33.0	31.0	34.0	31.0	34.0
2	31.527715932158994	31.0	31.0	33.0	30.0	34.0
3	32.51932536487845	33.0	31.0	34.0	31.0	34.0
4	36.37116295015158	37.0	37.0	37.0	35.0	37.0
5	36.538841402581596	37.0	37.0	37.0	35.0	37.0
6	36.34906127338631	37.0	37.0	37.0	35.0	37.0
7	36.45344790394712	37.0	37.0	37.0	35.0	37.0
8	36.660206581486634	37.0	37.0	37.0	35.0	37.0
9	38.7647338091808	39.0	39.0	39.0	39.0	39.0
10	38.29673313893693	39.0	39.0	39.0	37.0	39.0
11	38.02955139884518	39.0	38.0	39.0	37.0	39.0
12	37.71820578797952	39.0	37.0	39.0	35.0	39.0
13	37.63509265928885	39.0	37.0	39.0	35.0	39.0
14	38.431150739386844	40.0	38.0	40.0	35.0	40.0
15	38.46294495140732	40.0	38.0	40.0	35.0	40.0
16	38.49220937802139	40.0	38.0	40.0	35.0	40.0
17	38.45623095679238	40.0	38.0	40.0	35.0	40.0
18	38.37835266383418	40.0	38.0	40.0	35.0	40.0
19	38.37660868158409	40.0	38.0	40.0	35.0	40.0
20	38.31804130859337	40.0	38.0	40.0	35.0	40.0
21	38.275658013844	40.0	38.0	40.0	35.0	40.0
22	38.292873458728	40.0	38.0	40.0	35.0	40.0
23	38.209283647975596	40.0	38.0	40.0	35.0	40.0
24	38.17197436894999	40.0	38.0	40.0	35.0	40.0
25	38.08482148016039	40.0	38.0	40.0	34.0	40.0
26	37.953683837492825	40.0	38.0	40.0	34.0	40.0
27	37.86954704611201	40.0	37.0	40.0	34.0	40.0
28	37.69356084389096	40.0	37.0	40.0	34.0	40.0
29	37.76005173204113	40.0	37.0	40.0	34.0	40.0
30	37.876631793441625	40.0	38.0	40.0	34.0	40.0
31	37.88775726386422	40.0	38.0	40.0	34.0	40.0
32	37.87135651195846	40.0	37.0	40.0	34.0	40.0
33	37.773204304968665	40.0	37.0	40.0	34.0	40.0
34	37.49432892795649	40.0	37.0	40.0	33.0	40.0
35	37.46002226442276	40.0	37.0	40.0	33.0	40.0
36	37.38583127573602	40.0	36.0	40.0	33.0	40.0
37	37.24749043746894	40.0	36.0	40.0	33.0	40.0
38	37.1558076246018	40.0	35.0	40.0	33.0	40.0
39	37.02712080182738	40.0	35.0	40.0	33.0	40.0
40	36.80650078772914	40.0	35.0	40.0	32.0	40.0
41	36.67755588254554	40.0	35.0	40.0	32.0	40.0
42	36.57309413844773	39.0	35.0	40.0	32.0	40.0
43	36.43256152569076	39.0	35.0	40.0	31.0	40.0
44	36.14716956784678	39.0	35.0	40.0	31.0	40.0
45	36.10707049501747	39.0	35.0	40.0	30.0	40.0
46	35.923286929859366	39.0	35.0	40.0	30.0	40.0
47	35.85477953213896	38.0	35.0	40.0	30.0	40.0
48	35.75387412511989	38.0	35.0	40.0	30.0	40.0
49	35.577079007885	37.0	35.0	40.0	30.0	40.0
50	35.09273440238514	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	6.0
15	7.0
16	32.0
17	54.0
18	115.0
19	237.0
20	417.0
21	679.0
22	1144.0
23	1743.0
24	2400.0
25	3503.0
26	4630.0
27	6090.0
28	8453.0
29	10961.0
30	13363.0
31	16487.0
32	20831.0
33	27991.0
34	52140.0
35	64864.0
36	85685.0
37	154635.0
38	366954.0
39	195005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.66988563482495	0.23131117419792224	0.0660613927975748	0.03274179817955602
8	99.37848363102691	0.5253132619690533	0.07135786015015004	0.024845246853898394
9	98.93232848112724	0.6021601882845994	0.2042510409965833	0.26126028959157493
10	60.038057525413414	29.26587110517051	3.461578462830355	7.234492906585724
11	32.6725589063469	26.802724888003794	21.422573351257864	19.102142854391445
12	29.965967789774545	17.94915006143902	26.893246330029623	25.191635818756815
13	23.641119076142015	18.54880646515695	30.76708255170315	27.042991906997887
14	25.879598778153127	19.022695844102817	30.5677427804335	24.52996259731055
15	26.03011475037268	18.974353542084764	28.71157172187191	26.283959985670645
16	28.61421302199093	19.99291236368819	26.415890172452976	24.9769844418679
17	30.31784582079836	20.239342544692555	22.832300361700568	26.610511272808512
18	27.258991475576543	21.332918603889723	25.03023801361288	26.377851906920846
19	28.463697049771387	21.013493472826234	24.533718274160556	25.98909120324182
20	27.818491026821313	22.010384927987303	23.726632949034503	26.444491096156884
21	28.468126822466267	21.630580069104454	25.744394411552847	24.156898696876432
22	29.433817269950346	21.71455315149437	23.494647678991708	25.356981899563568
23	28.03853517046921	23.096642232297278	24.38734317641666	24.47747942081685
24	29.383452680397674	21.69895264765588	23.750322603011476	25.167272068934963
25	29.176890453647243	22.69777009094516	23.21412750811804	24.91121194728956
26	27.48895445808472	22.298127554341754	23.11734660467553	27.095571382897997
27	27.158165997064792	20.797012407215522	23.3380648441683	28.70675675155138
28	29.794458546957514	21.888951376503716	22.322683902976422	25.993906173562348
29	30.26035507517132	22.247762964789086	22.240733108121123	25.251148851918476
30	28.434999826661066	21.581948868867173	23.13227301266915	26.85077829180261
31	30.23512463069178	22.961630464509817	21.59851236676977	25.204732538028633
32	31.117901289256455	22.778083795891483	22.8574345067737	23.246580408078366
33	31.556448786049685	22.110825208873415	22.8215148281826	23.511211176894307
34	28.992380790964805	23.981152280177344	23.88321578385791	23.143251144999944
35	28.777922012888713	24.110963880018645	25.288320422792914	21.82279368429973
36	29.76807250960105	23.347405886590114	22.771920633881212	24.112600969927623
37	29.156282380675407	25.005200167946164	21.500479571043922	24.338037880334507
38	29.868994287519214	24.74018420150458	22.067297877175886	23.32352363380032
39	29.390193638846412	23.926357917929796	21.6218168231211	25.06163162010269
40	29.040819392389267	24.14332048057256	22.34955143736494	24.466308689673237
41	27.49348053018601	24.177314171035448	23.05234450534847	25.276860793430068
42	25.95191963236739	23.869541268147625	22.830952170010825	27.347586929474165
43	26.342702623581026	22.614856302025753	25.646265316420592	25.396175757972628
44	26.895557515783473	22.196916878204362	24.046443277723636	26.86108232828853
45	27.14824715820452	22.247474066569854	24.419892375783395	26.184386399442232
46	28.023030966037126	23.069100602063887	23.320442052795187	25.5874263791038
47	27.10635691641597	23.33825744298112	23.517566937717397	26.037818702885517
48	27.6526634489825	23.76823429260382	23.53528602849692	25.04381622991676
49	27.232027641781613	23.31071581274773	24.640803214088987	24.816453331381666
50	26.625533980208544	23.31447148959774	24.490479840682262	25.56951468951145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	6.0
2	12.0
3	14.0
4	16.0
5	31.5
6	47.0
7	631.0
8	1215.0
9	1918.0
10	2621.0
11	2999.5
12	3378.0
13	3004.0
14	2630.0
15	2473.0
16	2316.0
17	2775.5
18	3235.0
19	2999.0
20	2763.0
21	3916.0
22	5069.0
23	5579.0
24	6089.0
25	6844.0
26	7599.0
27	9747.5
28	11896.0
29	13330.5
30	14765.0
31	14697.5
32	14630.0
33	16228.5
34	17827.0
35	19597.5
36	21368.0
37	23730.5
38	26093.0
39	26696.5
40	27300.0
41	28747.5
42	30195.0
43	34597.5
44	39000.0
45	41615.5
46	44231.0
47	47909.5
48	51588.0
49	54790.5
50	57993.0
51	62464.0
52	66935.0
53	77298.5
54	87662.0
55	90495.0
56	93328.0
57	91501.5
58	89675.0
59	86731.5
60	83788.0
61	76912.5
62	70037.0
63	59142.5
64	48248.0
65	39826.0
66	31404.0
67	27343.0
68	23282.0
69	20557.0
70	17832.0
71	19063.5
72	20295.0
73	14203.0
74	8111.0
75	5193.0
76	2275.0
77	1689.5
78	1104.0
79	757.0
80	410.0
81	268.5
82	127.0
83	78.0
84	29.0
85	18.0
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1038428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.75209653473927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69108070839768	7.493252273869976
2	9.187518700518554	1.9757017596539557
3	3.5361940233998808	1.1406449851549072
4	1.7088837623409938	0.7349633271735522
5	0.9773199343343869	0.5254119139644186
6	0.6572083091495926	0.4239820310045513
7	0.5187503727406242	0.39043578595974193
8	0.4054699895377759	0.3487721975559911
9	0.34073859022212366	0.32972887936613154
>10	7.87205020104254	23.986784580653737
>50	3.532353270389754	26.263180122526137
>100	1.4879217771685016	26.748251795854227
>500	0.05753896902645565	4.3495017818647925
>1k	0.025173298949074347	4.094109322531827
>5k	0.0017980927820767391	1.1952792428660552
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	6449	0.6210348719410494	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5916	0.5697072883242749	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3722	0.358426390659728	No Hit
GAATTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2319	0.22331832346585415	No Hit
GAATTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2017	0.19423590272989558	No Hit
GAATTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1968	0.1895172318157831	No Hit
GAATTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1868	0.1798872911747372	No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1834	0.1766131113567816	No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1749	0.16842766181189261	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1670	0.16082000870546634	No Hit
GAATTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	1516	0.14598990011825566	No Hit
GAATTAGGGGTGCAGTGCTGAATTGGGGGCCAACCAGTAGAACACCCATT	1467	0.14127122920414317	No Hit
GAATTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	1423	0.137034055322083	No Hit
GAATTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	1414	0.13616736066438886	No Hit
GAATTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1406	0.1353969654131052	No Hit
GAATTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1384	0.1332783784720751	No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1349	0.129907899247709	No Hit
GAATTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1327	0.12778931230667895	No Hit
GAATTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1235	0.11892976691691673	No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA	1230	0.11844826988486443	No Hit
GAATTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1220	0.11748527582075984	No Hit
GAATTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA	1187	0.1143073954092147	No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1186	0.11421109600280423	No Hit
GAATTAGGGACTTGGGTGTATATTGTTAATTATTCTTTTGGATTCTTTTG	1180	0.11363329956434148	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1173	0.11295920371946827	No Hit
GAATTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1141	0.10987762271433359	No Hit
GAATTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	1137	0.10949242508869175	No Hit
GAATTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	1134	0.10920352686946037	No Hit
GAATTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	1065	0.10255886782713872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.03909755900264631	0.0	0.0	0.0	0.0
7	0.045453319825736596	0.0	0.0	0.0	0.0
8	0.05383136818344652	0.0	0.0	0.0	0.0
9	0.0936993224373765	0.0	0.0	0.0	0.0
10	0.16919805706317625	0.0	0.0	0.0	0.0
11	0.22967408428894445	0.0	0.0	0.0	0.0
12	0.2544230317364324	0.0	0.0	0.0	0.0
13	0.2713717272646731	0.0	0.0	0.0	0.0
14	0.2932316925198473	0.0	0.0	0.0	0.0
15	0.32481789782247783	0.0	0.0	0.0	0.0
16	0.35264842627510046	0.0	0.0	0.0	0.0
17	0.3792270624443871	0.0	0.0	0.0	0.0
18	0.4023389199828972	0.0	0.0	0.0	0.0
19	0.42265809473550403	0.0	0.0	0.0	0.0
20	0.44114758076631216	0.0	0.0	0.0	0.0
21	0.4619482525509713	0.0	0.0	0.0	0.0
22	0.4839045172125559	0.0	0.0	0.0	0.0
23	0.5092312610985066	0.0	0.0	0.0	0.0
24	0.5359061966742037	0.0	0.0	0.0	0.0
25	0.5580550601486093	0.0	0.0	0.0	0.0
26	0.5832855046281494	0.0	0.0	0.0	0.0
27	0.6083233502948688	0.0	0.0	0.0	0.0
28	0.6370205734051855	0.0	0.0	0.0	0.0
29	0.6607102273821585	0.0	0.0	0.0	0.0
30	0.6859406718616986	0.0	0.0	0.0	0.0
31	0.7073191400848206	0.0	0.0	0.0	0.0
32	0.7326458839707712	0.0	0.0	0.0	0.0
33	0.7521946634720944	0.0	0.0	0.0	0.0
34	0.7734768322888058	0.0	0.0	0.0	0.0
35	0.7959145939824427	0.0	0.0	0.0	0.0
36	0.8151744752645345	0.0	0.0	0.0	0.0
37	0.8351084523914994	0.0	0.0	0.0	0.0
38	0.8567758188338527	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	25	4.4425426E-5	44.0	43
TAATACA	25	4.4425426E-5	44.0	36
TCCGATT	20	7.8561314E-4	44.0	38
ACGTTAC	25	4.4425426E-5	44.0	18
ATAGCGA	25	4.4425426E-5	44.0	21
ATTCGTA	20	7.8561314E-4	44.0	33
TACGGTT	20	7.8561314E-4	44.0	40
TAAGTCG	20	7.8561314E-4	44.0	35
CGATTAT	20	7.8561314E-4	44.0	24
CGTTCGA	20	7.8561314E-4	44.0	14
TTATCGC	20	7.8561314E-4	44.0	40
AAAACGT	35	1.4458965E-7	44.0	28
ATCGTTT	20	7.8561314E-4	44.0	35
TTGCGAA	20	7.8561314E-4	44.0	44
CGTACTT	20	7.8561314E-4	44.0	40
TCGTAGT	30	2.5274803E-6	44.0	43
ACGTAAC	50	2.7284841E-11	44.0	11
GATCTAT	50	2.7284841E-11	44.0	9
ATACGGT	25	4.4425426E-5	44.0	10
TAGTGCC	20	7.8561314E-4	44.0	30
>>END_MODULE