FastQCFastQC Report
Fri 17 Jun 2016
SRR1527647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565752
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT96550.6166366065634915No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG87090.5562183538644689No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC56290.35950776368160475No Hit
GAATTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33680.2151043077064567No Hit
GAATTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC32970.21056974540029327No Hit
GAATTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG31550.2015006207879664No Hit
GAATTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG28430.18157409347074122No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT27790.17748660068772065No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG27350.17467644939939403No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24960.15941221853780163No Hit
GAATTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT24470.15628273187580152No Hit
GAATTAGGGGTGCAGTGCTGAATTGGGGGCCAACCAGTAGAACACCCATTTA24060.15366418181167898No Hit
GAATTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA22340.14267904495731126No Hit
GAATTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC22180.14165717176155612No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT21510.13737807775433147No Hit
GAATTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG20630.1317577751776782No Hit
GAATTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT20130.12856442144094338No Hit
GAATTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA19640.12543493477894327No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG19060.1217306444443309No Hit
GAATTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT18760.11981463220229001No Hit
GAATTAGGGACTTGGGTGTATATTGTTAATTATTCTTTTGGATTCTTTTGAA18600.11879275900653488No Hit
GAATTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA18150.11591874064347356No Hit
GAATTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT17900.11432206377510615No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT17520.1118951149351877No Hit
GAATTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC17440.11138417833731011No Hit
GAATTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG17350.11080937466469785No Hit
GAATTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA16680.1065302806574732No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16410.10480586963963641No Hit
GAATTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA16220.1035923952196772No Hit
GAATTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC16140.10308145862179961No Hit
GAATTAGGGGGACAGGGACTTCTAACCGACTGGCCTGCTGCTAATGCAGGAC15780.10078224393135055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA351.0198892E-746.00000414
TACGCAC351.0198892E-746.00000420
ATACGGT351.0198892E-746.00000410
AACGTTA301.8618375E-646.043
TAATACG206.312158E-446.021
CGAGTAT253.417727E-546.033
ATTCGAG253.417727E-546.046
TACGGTT301.8618375E-646.040
TATGCGT206.312158E-446.031
GTCGTAC405.6134013E-946.038
CGAATAG253.417727E-546.035
CATAACG206.312158E-446.028
ACGTAAC405.6134013E-946.011
GACGATA253.417727E-546.044
CGCAATT206.312158E-446.043
TATCGGG301.8618375E-646.031
AACCGAC1600.044.562524
TAGGGAT137250.043.7209475
AGGGATT45200.043.201336
TAGGGGA290300.043.0527085
Produced by FastQC (version 0.11.5)