##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565752
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937919287345633	33.0	31.0	33.0	31.0	34.0
2	31.168402786648205	31.0	31.0	33.0	30.0	34.0
3	32.2132285317215	33.0	31.0	34.0	31.0	34.0
4	36.17358751577517	37.0	35.0	37.0	35.0	37.0
5	36.35529253674911	37.0	37.0	37.0	35.0	37.0
6	36.13087385486335	37.0	36.0	37.0	35.0	37.0
7	36.31551612260434	37.0	35.0	37.0	35.0	37.0
8	36.55929930154967	37.0	37.0	37.0	35.0	37.0
9	38.685758664207356	39.0	39.0	39.0	38.0	39.0
10	38.071385506772465	39.0	38.0	39.0	37.0	39.0
11	37.683601234422824	39.0	38.0	39.0	35.0	39.0
12	37.36553937021955	39.0	37.0	39.0	35.0	39.0
13	37.281524149418296	39.0	37.0	39.0	34.0	39.0
14	38.113775361615374	40.0	38.0	40.0	34.0	40.0
15	38.16745691527139	40.0	38.0	40.0	34.0	40.0
16	38.19272783940241	40.0	38.0	40.0	34.0	40.0
17	38.14831212094891	40.0	38.0	40.0	34.0	40.0
18	38.12421124162702	40.0	38.0	40.0	34.0	40.0
19	38.127347753667244	40.0	38.0	40.0	34.0	40.0
20	38.12977214782418	40.0	38.0	40.0	34.0	40.0
21	38.077442660140306	40.0	38.0	40.0	34.0	40.0
22	38.07826910008737	40.0	38.0	40.0	34.0	40.0
23	38.03090974815935	40.0	38.0	40.0	34.0	40.0
24	37.97033246644424	40.0	38.0	40.0	34.0	40.0
25	37.89407198585727	40.0	37.0	40.0	34.0	40.0
26	37.750184575845985	40.0	37.0	40.0	34.0	40.0
27	37.67716598797255	40.0	37.0	40.0	33.0	40.0
28	37.543919471282805	40.0	37.0	40.0	33.0	40.0
29	37.47332591623705	40.0	36.0	40.0	33.0	40.0
30	37.35363071546452	40.0	36.0	40.0	33.0	40.0
31	37.24440268957025	40.0	36.0	40.0	33.0	40.0
32	37.11863947802717	39.0	36.0	40.0	32.0	40.0
33	36.987384975398406	39.0	35.0	40.0	32.0	40.0
34	36.877288995958494	39.0	35.0	40.0	31.0	40.0
35	36.72659654913422	39.0	35.0	40.0	31.0	40.0
36	36.67811505270311	39.0	35.0	40.0	31.0	40.0
37	36.500238862859504	39.0	35.0	40.0	31.0	40.0
38	36.41332216085306	39.0	35.0	40.0	31.0	40.0
39	36.287139981299724	39.0	35.0	40.0	31.0	40.0
40	36.05266478982623	38.0	35.0	40.0	30.0	40.0
41	35.908853381633875	38.0	35.0	40.0	30.0	40.0
42	35.787880200695895	38.0	35.0	40.0	30.0	40.0
43	35.65829518340069	38.0	35.0	40.0	29.0	40.0
44	35.49851061981719	38.0	34.0	40.0	29.0	40.0
45	35.406557360297164	38.0	34.0	40.0	29.0	40.0
46	35.21192053403093	37.0	34.0	40.0	28.0	40.0
47	35.069579984569714	37.0	34.0	40.0	28.0	40.0
48	34.85273657641823	37.0	34.0	40.0	27.0	40.0
49	35.092312831150785	37.0	34.0	40.0	28.0	40.0
50	35.16334387565847	37.0	34.0	40.0	29.0	40.0
51	35.103828703396196	36.0	34.0	40.0	29.0	40.0
52	34.6400215359776	36.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	8.0
16	33.0
17	84.0
18	211.0
19	410.0
20	749.0
21	1363.0
22	2183.0
23	3565.0
24	5093.0
25	7116.0
26	9599.0
27	12951.0
28	16821.0
29	20743.0
30	25763.0
31	32103.0
32	41570.0
33	57971.0
34	97001.0
35	112454.0
36	165258.0
37	259529.0
38	514756.0
39	178414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.73188602026374	0.20348050010474203	0.04764483775208334	0.01698864187942918
8	99.40820768550832	0.5056994977493243	0.06169559419371651	0.024397222548653938
9	98.94842861449322	0.5947301999294907	0.18968521196204763	0.2671559736152341
10	59.912361600049046	29.425860544964976	3.46727962027192	7.194498234714055
11	32.52967264292174	26.87909707284423	21.34750586299746	19.243724421236568
12	29.875484751097236	17.891275246654644	27.119875944594035	25.113364057654085
13	23.689447626444036	18.402658914055355	30.784760294095104	27.123133165405505
14	25.852497713558726	19.032963074612073	30.546663839484157	24.567875372345046
15	26.04243839381971	18.940739018695172	28.70007510767989	26.31674747980523
16	28.607787184688256	19.925058374506307	26.414336369999848	25.052818070805593
17	30.428445884150236	20.114360384019946	22.83624737506323	26.620946356766588
18	27.491071382952086	21.1463884446579	24.94628779014812	26.41625238224189
19	28.457380223688045	20.913337488950994	24.613987400303497	26.015294887057465
20	27.85734905655557	21.881179139480583	23.70228490846571	26.559186895498136
21	28.490144033026944	21.512091314588773	25.812900127223216	24.184864525161075
22	29.541459950234778	21.66288147803739	23.368962645425327	25.426695926302507
23	28.296115859982933	22.95618974141499	24.184161987338992	24.563532411263086
24	29.378535042586567	21.59435210684706	23.701710104793097	25.325402745773278
25	29.21024530066064	22.528408074841995	23.203802390161403	25.05754423433596
26	27.535011930369564	22.083062962716955	23.159734108594463	27.222190998319018
27	27.183104348581384	20.68629003826915	23.26166595987104	28.868939653278424
28	29.76116268732213	21.78735840669531	22.294143644715128	26.157335261267427
29	30.326258564574722	22.177266897950634	22.24068690316219	25.25578763431246
30	28.417846504427263	21.619324133068325	23.133612475027974	26.82921688747643
31	30.340245453941623	22.840718070294656	21.60987180600759	25.209164669756127
32	31.03799324541818	22.684690806717793	22.97566919920907	23.30164674865496
33	31.602769787297095	21.977171352806828	22.731186037124655	23.688872822771422
34	28.927761229109077	23.800193134033997	23.86795610032751	23.404089536529412
35	28.872771677762504	24.054511825627557	25.151556568345434	21.9211599282645
36	29.935711402572057	23.251447227913488	22.71400579402102	24.09883557549344
37	29.324950566884155	24.835925485006566	21.42963892110628	24.409485027003
38	29.861497861730342	24.645410001072968	22.07195009171312	23.421142045483574
39	29.444126528339098	23.783715428752448	21.57800213571498	25.194155907193476
40	29.009127882321085	24.099538113315518	22.400610058297865	24.490723946065533
41	27.36052708219437	24.17637020422136	23.096122502158707	25.366980211425567
42	25.99409101824555	23.859334045238327	22.750090691246122	27.396484245270003
43	26.424363500733193	22.507331940179544	25.609036424670062	25.4592681344172
44	26.73003132041345	22.144758556910674	24.097430499849274	27.027779622826603
45	27.307581277239308	22.163854812256346	24.3673327576781	26.161231152826247
46	27.933287008415125	22.93824309341454	23.389400109340432	25.739069788829905
47	26.99060898533101	23.270862818632835	23.533420362867172	26.205107833168984
48	27.74986076977708	23.66000490499134	23.564204292889297	25.025930032342288
49	27.242436861009917	23.18604734338516	24.736356715495177	24.83515908010975
50	26.632761765592505	23.29328016186471	24.46881753943153	25.605140533111246
51	25.920899350599587	23.698005814458483	23.881112717722857	26.499982117219073
52	26.175281909267877	24.528533254308474	23.47996362131423	25.81622121510942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	8.0
2	16.0
3	17.5
4	19.0
5	33.0
6	47.0
7	891.5
8	1736.0
9	2665.0
10	3594.0
11	4219.0
12	4844.0
13	4168.0
14	3232.5
15	2973.0
16	3567.0
17	4161.0
18	3784.5
19	3408.0
20	4794.5
21	6181.0
22	6894.5
23	7608.0
24	8254.5
25	8901.0
26	12774.0
27	16647.0
28	18034.5
29	19422.0
30	18206.5
31	16991.0
32	20257.5
33	23524.0
34	25871.5
35	28219.0
36	30345.5
37	32472.0
38	35346.5
39	38493.0
40	38765.0
41	43532.0
42	48299.0
43	51521.0
44	54743.0
45	58829.0
46	62915.0
47	68344.5
48	73774.0
49	81109.5
50	88445.0
51	92183.0
52	95921.0
53	104970.5
54	114020.0
55	133462.0
56	152904.0
57	136971.5
58	121039.0
59	124420.5
60	127802.0
61	116083.0
62	104364.0
63	93944.0
64	68301.5
65	53079.0
66	43477.0
67	33875.0
68	32700.5
69	31526.0
70	32876.5
71	34227.0
72	24990.0
73	15753.0
74	10239.5
75	4726.0
76	3495.0
77	2264.0
78	1537.5
79	811.0
80	587.5
81	364.0
82	239.0
83	114.0
84	63.5
85	13.0
86	9.5
87	6.0
88	4.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1565752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.276719475865985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.38678577743126	6.529584664651034
2	9.251109957603829	1.7163990383416232
3	4.008706125016897	1.1156292654890252
4	2.2108559889554185	0.8203796324431072
5	1.2038768939684743	0.5584014114411197
6	0.776498306289569	0.4322014176560036
7	0.5451030877806006	0.35397379015382874
8	0.39488435401916555	0.2930585102115481
9	0.32924642063382253	0.27488940143876506
>10	4.686337394234408	11.86897729734149
>50	3.671352145636006	24.648626549145337
>100	2.4175013847201834	38.90391040047438
>500	0.07505202248205639	4.7061120121625155
>1k	0.04062448923340667	6.245305806115583
>5k	0.0020656519949189835	1.532550802934637
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9655	0.6166366065634915	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8709	0.5562183538644689	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5629	0.35950776368160475	No Hit
GAATTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3368	0.2151043077064567	No Hit
GAATTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3297	0.21056974540029327	No Hit
GAATTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3155	0.2015006207879664	No Hit
GAATTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2843	0.18157409347074122	No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2779	0.17748660068772065	No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2735	0.17467644939939403	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2496	0.15941221853780163	No Hit
GAATTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2447	0.15628273187580152	No Hit
GAATTAGGGGTGCAGTGCTGAATTGGGGGCCAACCAGTAGAACACCCATTTA	2406	0.15366418181167898	No Hit
GAATTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	2234	0.14267904495731126	No Hit
GAATTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2218	0.14165717176155612	No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2151	0.13737807775433147	No Hit
GAATTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2063	0.1317577751776782	No Hit
GAATTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2013	0.12856442144094338	No Hit
GAATTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	1964	0.12543493477894327	No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	1906	0.1217306444443309	No Hit
GAATTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1876	0.11981463220229001	No Hit
GAATTAGGGACTTGGGTGTATATTGTTAATTATTCTTTTGGATTCTTTTGAA	1860	0.11879275900653488	No Hit
GAATTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1815	0.11591874064347356	No Hit
GAATTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	1790	0.11432206377510615	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1752	0.1118951149351877	No Hit
GAATTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1744	0.11138417833731011	No Hit
GAATTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1735	0.11080937466469785	No Hit
GAATTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1668	0.1065302806574732	No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1641	0.10480586963963641	No Hit
GAATTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1622	0.1035923952196772	No Hit
GAATTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1614	0.10308145862179961	No Hit
GAATTAGGGGGACAGGGACTTCTAACCGACTGGCCTGCTGCTAATGCAGGAC	1578	0.10078224393135055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0366597008977156	0.0	0.0	0.0	0.0
7	0.04247160469857295	0.0	0.0	0.0	0.0
8	0.04898604632151196	0.0	0.0	0.0	0.0
9	0.08628441796657453	0.0	0.0	0.0	0.0
10	0.1520036378685769	0.0	0.0	0.0	0.0
11	0.2083343977845789	0.0	0.0	0.0	0.0
12	0.23158201298800832	0.0	0.0	0.0	0.0
13	0.24716557922327417	0.0	0.0	0.0	0.0
14	0.2683055809604586	0.0	0.0	0.0	0.0
15	0.29596002432058205	0.0	0.0	0.0	0.0
16	0.32303966400809325	0.0	0.0	0.0	0.0
17	0.34405193159580827	0.0	0.0	0.0	0.0
18	0.3639145918382988	0.0	0.0	0.0	0.0
19	0.3817973727640137	0.0	0.0	0.0	0.0
20	0.4001910902876062	0.0	0.0	0.0	0.0
21	0.4182654724375252	0.0	0.0	0.0	0.0
22	0.4386390692778933	0.0	0.0	0.0	0.0
23	0.4612480137339757	0.0	0.0	0.0	0.0
24	0.4866671094783848	0.0	0.0	0.0	0.0
25	0.5061465672724672	0.0	0.0	0.0	0.0
26	0.5308631251947946	0.0	0.0	0.0	0.0
27	0.5522585952309178	0.0	0.0	0.0	0.0
28	0.5769751531532452	0.0	0.0	0.0	0.0
29	0.5958798072747152	0.0	0.0	0.0	0.0
30	0.6153592650687976	0.0	0.0	0.0	0.0
31	0.6343916533397371	0.0	0.0	0.0	0.0
32	0.654382047731697	0.0	0.0	0.0	0.0
33	0.6721370945079425	0.0	0.0	0.0	0.0
34	0.6883593314905553	0.0	0.0	0.0	0.0
35	0.7082858588077805	0.0	0.0	0.0	0.0
36	0.7254661019114138	0.0	0.0	0.0	0.0
37	0.7437959523602716	0.0	0.0	0.0	0.0
38	0.7625728723322723	0.0	0.0	0.0	0.0
39	0.7805195203327219	0.0	0.0	0.0	0.0
40	0.7999989781268042	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTA	35	1.0198892E-7	46.000004	14
TACGCAC	35	1.0198892E-7	46.000004	20
ATACGGT	35	1.0198892E-7	46.000004	10
AACGTTA	30	1.8618375E-6	46.0	43
TAATACG	20	6.312158E-4	46.0	21
CGAGTAT	25	3.417727E-5	46.0	33
ATTCGAG	25	3.417727E-5	46.0	46
TACGGTT	30	1.8618375E-6	46.0	40
TATGCGT	20	6.312158E-4	46.0	31
GTCGTAC	40	5.6134013E-9	46.0	38
CGAATAG	25	3.417727E-5	46.0	35
CATAACG	20	6.312158E-4	46.0	28
ACGTAAC	40	5.6134013E-9	46.0	11
GACGATA	25	3.417727E-5	46.0	44
CGCAATT	20	6.312158E-4	46.0	43
TATCGGG	30	1.8618375E-6	46.0	31
AACCGAC	160	0.0	44.5625	24
TAGGGAT	13725	0.0	43.720947	5
AGGGATT	4520	0.0	43.20133	6
TAGGGGA	29030	0.0	43.052708	5
>>END_MODULE