FastQCFastQC Report
Fri 17 Jun 2016
SRR1527646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578304
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT89960.569978914074855No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG85740.5432413527431978No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC51860.3285805522890394No Hit
GAATTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC34080.21592798345565875No Hit
GAATTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC30380.19248509792790236No Hit
GAATTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG28250.17898959896192368No Hit
GAATTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG27110.17176665585337172No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT25790.16340324804346945No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG24300.15396273468229188No Hit
GAATTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT23680.1500344673776408No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23640.14978103077734076No Hit
GAATTAGGGGTGCAGTGCTGAATTGGGGGCCAACCAGTAGAACACCCATTTA22620.14331839746968897No Hit
GAATTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC20880.132293905356636No Hit
GAATTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA20860.13216718705648595No Hit
GAATTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG20290.12855571550220996No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT20210.12804884230160982No Hit
GAATTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA20050.12703509590040954No Hit
GAATTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT19310.12234651879485829No Hit
GAATTAGGGACTTGGGTGTATATTGTTAATTATTCTTTTGGATTCTTTTGAA18630.11803809658975711No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG18270.11575716718705649No Hit
GAATTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA17860.11315944203398078No Hit
GAATTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT17500.11087851263128015No Hit
GAATTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT17100.10834414662827949No Hit
GAATTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG16910.10714032277685415No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT16520.10466931592392847No Hit
GAATTAGGGGGACAGGGACTTCTAACCGACTGGCCTGCTGCTAATGCAGGAC16330.10346549207250315No Hit
GAATTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA16260.10302197802197803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT351.0198892E-746.00000440
TCGCATC301.8618484E-646.018
CCGATTA253.41774E-546.014
CCGATGC206.312173E-446.021
ATAGCGA206.312173E-446.021
CGATTAC206.312173E-446.026
ATCGTCA253.41774E-546.010
ATAAGGT206.312173E-446.034
CGTACTG253.41774E-546.017
TCCCGTA405.6134013E-946.031
TATAGGC206.312173E-446.023
CCTATCG253.41774E-546.024
TCGACGA253.41774E-546.021
CGTTTAG301.8618484E-646.013
GCGTAAC253.41774E-546.035
GTCGGTA206.312173E-446.041
TAGGGAT140200.043.6212545
AGGGATT48950.043.1807986
TAGGGAG167050.043.1774945
TTAGGGA487550.043.0468674
Produced by FastQC (version 0.11.5)