##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527646_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1578304
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.880384894164877	31.0	31.0	33.0	30.0	34.0
2	31.123253188232432	31.0	31.0	33.0	30.0	34.0
3	32.16835413203033	33.0	31.0	34.0	31.0	34.0
4	36.16323344552127	37.0	35.0	37.0	35.0	37.0
5	36.35973614715543	37.0	37.0	37.0	35.0	37.0
6	36.04835126819675	37.0	35.0	37.0	35.0	37.0
7	36.25678576497303	37.0	35.0	37.0	35.0	37.0
8	36.52249946778314	37.0	37.0	37.0	35.0	37.0
9	38.637531172701834	39.0	39.0	39.0	38.0	39.0
10	37.97488253213576	39.0	38.0	39.0	35.0	39.0
11	37.64909421759053	39.0	38.0	39.0	35.0	39.0
12	37.31802174992904	39.0	37.0	39.0	35.0	39.0
13	37.08485754328697	39.0	37.0	39.0	34.0	39.0
14	38.058979765621835	40.0	38.0	40.0	34.0	40.0
15	38.12287239974048	40.0	38.0	40.0	34.0	40.0
16	38.17587929828474	40.0	38.0	40.0	34.0	40.0
17	38.16586855257289	40.0	38.0	40.0	34.0	40.0
18	38.154889045456386	40.0	38.0	40.0	34.0	40.0
19	38.11100586452293	40.0	38.0	40.0	34.0	40.0
20	38.12494044239893	40.0	38.0	40.0	34.0	40.0
21	38.13393300656907	40.0	38.0	40.0	34.0	40.0
22	38.01662734175419	40.0	38.0	40.0	34.0	40.0
23	37.99339100705568	40.0	38.0	40.0	34.0	40.0
24	37.986145254653096	40.0	38.0	40.0	34.0	40.0
25	37.874291011110664	40.0	37.0	40.0	34.0	40.0
26	37.684511348890965	40.0	37.0	40.0	33.0	40.0
27	37.619132942703054	40.0	37.0	40.0	33.0	40.0
28	37.500545522282145	40.0	37.0	40.0	33.0	40.0
29	37.432502863833584	40.0	36.0	40.0	33.0	40.0
30	37.30176315842829	40.0	36.0	40.0	33.0	40.0
31	37.21061848667937	40.0	36.0	40.0	33.0	40.0
32	37.08894864360732	39.0	36.0	40.0	32.0	40.0
33	36.79112198917319	39.0	35.0	40.0	31.0	40.0
34	36.785931607599046	39.0	35.0	40.0	31.0	40.0
35	36.70951920542557	39.0	35.0	40.0	31.0	40.0
36	36.51645880641499	39.0	35.0	40.0	31.0	40.0
37	36.42311430497547	39.0	35.0	40.0	31.0	40.0
38	36.06007587891813	38.0	35.0	40.0	30.0	40.0
39	36.113104953164914	38.0	35.0	40.0	30.0	40.0
40	35.94604017983861	38.0	35.0	40.0	30.0	40.0
41	35.608786393495805	38.0	35.0	40.0	29.0	40.0
42	35.63190044503467	38.0	35.0	40.0	29.0	40.0
43	35.614083218442076	38.0	34.0	40.0	29.0	40.0
44	35.44706849884433	38.0	34.0	40.0	29.0	40.0
45	35.35393308260006	38.0	34.0	40.0	28.0	40.0
46	34.991703119297675	37.0	34.0	40.0	27.0	40.0
47	34.76115944710271	37.0	34.0	40.0	26.0	40.0
48	34.71897682575727	37.0	33.0	40.0	26.0	40.0
49	34.58283765358258	36.0	33.0	40.0	27.0	40.0
50	34.43698045496939	36.0	33.0	39.0	26.0	40.0
51	34.692974230566485	36.0	34.0	40.0	27.0	40.0
52	34.4415587871538	36.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	6.0
15	8.0
16	30.0
17	77.0
18	211.0
19	434.0
20	789.0
21	1463.0
22	2327.0
23	3576.0
24	5562.0
25	7520.0
26	10011.0
27	13500.0
28	17636.0
29	21749.0
30	27077.0
31	33817.0
32	43754.0
33	61819.0
34	101243.0
35	117217.0
36	176230.0
37	275159.0
38	505935.0
39	151151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.72578159847532	0.20471341389238068	0.05087739751023884	0.018627590122055067
8	99.39606058148493	0.5158068407607153	0.06348586837516726	0.024646709379181703
9	98.9228944487247	0.6020386440128138	0.19825078058472892	0.2768161266777503
10	59.82529347958315	29.4970423948745	3.465808868253518	7.211855257288836
11	32.5297281132152	26.825630550261547	21.367619926199264	19.277021410323993
12	29.80382739953773	17.941347167592557	27.110049774948298	25.144775657921414
13	23.96509164267467	18.322452455293785	30.6907921414379	27.02166376059365
14	25.79800849519484	18.970299764810836	30.650749158590486	24.580942581403836
15	26.063990207209763	18.890720672316615	28.693458294473057	26.351830826000565
16	28.599496674911805	19.809301630104212	26.517388285146588	25.0738134098374
17	30.30708912858359	20.14630894935323	22.85041411540489	26.696187806658283
18	27.439073841287865	21.19762732654799	25.0002534366003	26.363045395563844
19	28.53353979968371	20.862774218401526	24.584744130408335	26.018941851506426
20	27.876632131705932	21.861314423583796	23.68865567089737	26.5733977738129
21	28.48481661327602	21.459744130408335	25.850976744657554	24.204462511658082
22	29.642198207696364	21.65413000283849	23.39821732695349	25.305454462511655
23	28.21243562710352	22.866127184623494	24.228348911236367	24.69308827703662
24	29.357588905559385	21.557253862373788	23.728508576294555	25.356648655772275
25	29.265718137950607	22.526965654271926	23.232976663557846	24.974339544219617
26	27.5731418028466	22.01591074976684	23.199649750618384	27.211297696768177
27	27.15174009569766	20.62581099712096	23.295828940432262	28.92661996674912
28	29.856288775799843	21.724458659421757	22.311924698917316	26.107327865861073
29	30.360120737196382	22.188120919670734	22.196864482381088	25.254893860751793
30	28.496601415189975	21.56536383358339	23.10486446210616	26.833170289120474
31	30.271734722841735	22.7920603381858	21.60787782328373	25.328327115688737
32	31.08342879445278	22.682132212805644	22.975104922752525	23.259334069989052
33	31.53492609788735	21.962942500304123	22.8016909289972	23.70044047281132
34	28.949872774826652	23.747326243866834	23.9059141965046	23.396886784801914
35	28.865478386926725	24.02407901139451	25.237723531081464	21.8727190705973
36	30.067274745549653	23.153524289363773	22.722048477352903	24.057152487733667
37	29.39503416325372	24.81727221118365	21.419194274360326	24.368499351202303
38	29.959374112971897	24.592790742467866	22.040367381695795	23.407467762864442
39	29.436787843153155	23.78325088195937	21.55034771501561	25.22961355987186
40	29.028754916670046	24.068113620696646	22.318894205425572	24.584237257207736
41	27.612614553343334	24.106699343092334	23.081294858278252	25.19939124528608
42	25.95558270143141	23.81417014719598	22.812588702810103	27.41765844856251
43	26.44896040306557	22.46398665909736	25.64379232391225	25.443260613924824
44	26.827594683913876	22.098594440614736	24.073815944203396	26.999994931267995
45	27.263695713880214	22.089217286403635	24.393399497181786	26.25368750253437
46	28.07925469364584	22.856813389562465	23.426855662787396	25.6370762540043
47	27.277698086046794	23.253441669032075	23.515305036292123	25.95355520862901
48	27.775701005636428	23.610723916305094	23.566245792952433	25.04732928510604
49	27.275037001743645	23.145984550504846	24.656530047443333	24.922448400308177
50	26.655257795709826	23.28195328656583	24.395553708284336	25.66723520944001
51	25.9363215198086	23.686564818944895	23.911046287660678	26.466067373585823
52	26.260530290742466	24.404677425895137	23.51758596569482	25.817206317667573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	7.5
2	15.0
3	19.5
4	24.0
5	38.5
6	53.0
7	811.5
8	1570.0
9	2510.5
10	3451.0
11	4039.0
12	4627.0
13	4083.0
14	3311.5
15	3084.0
16	3542.5
17	4001.0
18	3771.5
19	3542.0
20	4815.5
21	6089.0
22	6856.0
23	7623.0
24	8299.5
25	8976.0
26	12816.0
27	16656.0
28	17954.5
29	19253.0
30	18205.5
31	17158.0
32	20123.0
33	23088.0
34	25699.0
35	28310.0
36	30718.0
37	33126.0
38	35894.0
39	39044.0
40	39426.0
41	43948.5
42	48471.0
43	51870.5
44	55270.0
45	59559.5
46	63849.0
47	69088.5
48	74328.0
49	81461.0
50	88594.0
51	92772.5
52	96951.0
53	106515.0
54	116079.0
55	134885.0
56	153691.0
57	138564.5
58	123438.0
59	126011.0
60	128584.0
61	117011.5
62	105439.0
63	94348.5
64	68488.5
65	53719.0
66	44150.0
67	34581.0
68	33265.0
69	31949.0
70	32731.5
71	33514.0
72	24676.5
73	15839.0
74	10357.0
75	4875.0
76	3598.5
77	2322.0
78	1566.0
79	810.0
80	565.5
81	321.0
82	222.5
83	124.0
84	70.0
85	16.0
86	11.0
87	6.0
88	4.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1578304.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.475592740373841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60176405606595	7.50070919743685
2	9.46321505936349	1.9826557395292919
3	3.971777853337599	1.2482018174040286
4	1.9791599249822205	0.8293149336873035
5	1.152893314527336	0.6038620418044048
6	0.7579459781344531	0.47639600316844966
7	0.5415704253062682	0.3971289851017662
8	0.36998916902673784	0.3100684682458756
9	0.26576681936668733	0.2505658467230924
>10	4.473147987340395	12.892354963198782
>50	3.2675903159267445	24.60507365008328
>100	2.056674549847901	37.079877539012266
>500	0.062114332042386276	4.429255030663298
>1k	0.03450796224577015	5.898881718385978
>5k	0.0018822524861329174	1.4956540655553348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	8996	0.569978914074855	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8574	0.5432413527431978	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5186	0.3285805522890394	No Hit
GAATTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3408	0.21592798345565875	No Hit
GAATTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3038	0.19248509792790236	No Hit
GAATTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2825	0.17898959896192368	No Hit
GAATTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2711	0.17176665585337172	No Hit
GAATTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2579	0.16340324804346945	No Hit
GAATTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2430	0.15396273468229188	No Hit
GAATTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	2368	0.1500344673776408	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2364	0.14978103077734076	No Hit
GAATTAGGGGTGCAGTGCTGAATTGGGGGCCAACCAGTAGAACACCCATTTA	2262	0.14331839746968897	No Hit
GAATTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2088	0.132293905356636	No Hit
GAATTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	2086	0.13216718705648595	No Hit
GAATTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2029	0.12855571550220996	No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2021	0.12804884230160982	No Hit
GAATTAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2005	0.12703509590040954	No Hit
GAATTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1931	0.12234651879485829	No Hit
GAATTAGGGACTTGGGTGTATATTGTTAATTATTCTTTTGGATTCTTTTGAA	1863	0.11803809658975711	No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	1827	0.11575716718705649	No Hit
GAATTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1786	0.11315944203398078	No Hit
GAATTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1750	0.11087851263128015	No Hit
GAATTAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	1710	0.10834414662827949	No Hit
GAATTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1691	0.10714032277685415	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1652	0.10466931592392847	No Hit
GAATTAGGGGGACAGGGACTTCTAACCGACTGGCCTGCTGCTAATGCAGGAC	1633	0.10346549207250315	No Hit
GAATTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1626	0.10302197802197803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.035354405741859614	0.0	0.0	0.0	0.0
7	0.04099337009853615	0.0	0.0	0.0	0.0
8	0.047456003406187906	0.0	0.0	0.0	0.0
9	0.08186002189692226	0.0	0.0	0.0	0.0
10	0.1488940026762905	0.0	0.0	0.0	0.0
11	0.19932788613600422	0.0	0.0	0.0	0.0
12	0.22194710271278537	0.0	0.0	0.0	0.0
13	0.23689986213048944	0.0	0.0	0.0	0.0
14	0.2568579944041199	0.0	0.0	0.0	0.0
15	0.2857497668383277	0.0	0.0	0.0	0.0
16	0.31058655366773447	0.0	0.0	0.0	0.0
17	0.3328256153440655	0.0	0.0	0.0	0.0
18	0.35348069826852113	0.0	0.0	0.0	0.0
19	0.37451593609342687	0.0	0.0	0.0	0.0
20	0.39453742751713233	0.0	0.0	0.0	0.0
21	0.41310165848911234	0.0	0.0	0.0	0.0
22	0.43299643161266776	0.0	0.0	0.0	0.0
23	0.45561564818944894	0.0	0.0	0.0	0.0
24	0.47785470986578	0.0	0.0	0.0	0.0
25	0.4975594055391103	0.0	0.0	0.0	0.0
26	0.5192282348647662	0.0	0.0	0.0	0.0
27	0.5421008880418474	0.0	0.0	0.0	0.0
28	0.5645933863184786	0.0	0.0	0.0	0.0
29	0.580940047037833	0.0	0.0	0.0	0.0
30	0.6006447427111634	0.0	0.0	0.0	0.0
31	0.6181952272819431	0.0	0.0	0.0	0.0
32	0.6389770285065488	0.0	0.0	0.0	0.0
33	0.6560206398767284	0.0	0.0	0.0	0.0
34	0.672810814646608	0.0	0.0	0.0	0.0
35	0.6935926158712137	0.0	0.0	0.0	0.0
36	0.7115866144925186	0.0	0.0	0.0	0.0
37	0.7290103807631483	0.0	0.0	0.0	0.0
38	0.7489051538867038	0.0	0.0	0.0	0.0
39	0.7668357933579336	0.0	0.0	0.0	0.0
40	0.7856534609302137	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTT	35	1.0198892E-7	46.000004	40
TCGCATC	30	1.8618484E-6	46.0	18
CCGATTA	25	3.41774E-5	46.0	14
CCGATGC	20	6.312173E-4	46.0	21
ATAGCGA	20	6.312173E-4	46.0	21
CGATTAC	20	6.312173E-4	46.0	26
ATCGTCA	25	3.41774E-5	46.0	10
ATAAGGT	20	6.312173E-4	46.0	34
CGTACTG	25	3.41774E-5	46.0	17
TCCCGTA	40	5.6134013E-9	46.0	31
TATAGGC	20	6.312173E-4	46.0	23
CCTATCG	25	3.41774E-5	46.0	24
TCGACGA	25	3.41774E-5	46.0	21
CGTTTAG	30	1.8618484E-6	46.0	13
GCGTAAC	25	3.41774E-5	46.0	35
GTCGGTA	20	6.312173E-4	46.0	41
TAGGGAT	14020	0.0	43.621254	5
AGGGATT	4895	0.0	43.180798	6
TAGGGAG	16705	0.0	43.177494	5
TTAGGGA	48755	0.0	43.046867	4
>>END_MODULE