FastQCFastQC Report
Fri 17 Jun 2016
SRR1527645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175127
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG61390.522411620190839No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC59180.5036051422527097No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58020.49373386876482284No Hit
AGTCGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT54490.4636945623749603No Hit
AGTCGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC29170.2482284893462579No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT27810.236655272153563No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG24920.21206218561908627No Hit
AGTCGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC21000.178704089004848No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA20990.17861899181960758No Hit
AGTCGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC20720.1763213678181167No Hit
AGTCGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG20330.1730025775937409No Hit
AGTCGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG17580.14960085165262987No Hit
AGTCGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT17540.14926046291166825No Hit
AGTCGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG17200.14636715861349453No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG16310.13879350912709862No Hit
AGTCGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC16030.1364107879403673No Hit
AGTCGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT14770.12568854260007642No Hit
AGTCGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA13700.11658314377935321No Hit
AGTCGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC13400.1140302282221411No Hit
AGTCGTGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC12940.11011575770108252No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC12840.10926478584867849No Hit
AGTCGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA12760.10858400836675525No Hit
AGTCGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT12720.10824361962579364No Hit
AGTCGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA12710.10815852244055323No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC11790.10032958139843608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGA207.8565546E-444.026
TGATACG207.8565546E-444.024
CGTATAG207.8565546E-444.041
ATCTAGT254.4429016E-544.030
CGACGTA254.4429016E-544.029
TAGACTA254.4429016E-544.020
GCGAATA302.527766E-644.031
GCGCGTA254.4429016E-544.040
CACGATA207.8565546E-444.032
TAAGTCG254.4429016E-544.043
TATGCGC502.7284841E-1144.026
TACATAC254.4429016E-544.033
CCGTATA207.8565546E-444.040
ACGATAT207.8565546E-444.033
TCTAACG207.8565546E-444.033
CGAATTT254.4429016E-544.042
CGAATAC302.527766E-644.032
CCGCGTA207.8565546E-444.044
ATACGCC207.8565546E-444.026
GCGCTTA408.312782E-944.016
Produced by FastQC (version 0.11.5)