##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527645_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1175127
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.360147456402583	31.0	31.0	33.0	3.0	34.0
2	30.096375966172168	33.0	31.0	34.0	16.0	34.0
3	30.174809190836395	31.0	30.0	31.0	25.0	33.0
4	35.642597778793274	35.0	35.0	37.0	33.0	37.0
5	36.045948225170555	37.0	35.0	37.0	35.0	37.0
6	35.49299267228138	35.0	35.0	37.0	35.0	37.0
7	36.35618192757038	37.0	35.0	37.0	35.0	37.0
8	36.656986010873716	37.0	37.0	37.0	35.0	37.0
9	38.748837359706656	39.0	39.0	39.0	39.0	39.0
10	38.26980232774841	39.0	39.0	39.0	37.0	39.0
11	37.90546298400088	39.0	38.0	39.0	35.0	39.0
12	37.33481402435652	39.0	37.0	39.0	35.0	39.0
13	37.18464727642204	39.0	37.0	39.0	34.0	39.0
14	38.04742381036262	40.0	38.0	40.0	34.0	40.0
15	38.0674488799934	40.0	38.0	40.0	34.0	40.0
16	38.0737333071234	40.0	38.0	40.0	34.0	40.0
17	37.97157498721415	40.0	38.0	40.0	34.0	40.0
18	37.8265855520297	40.0	37.0	40.0	33.0	40.0
19	37.783724652739664	40.0	37.0	40.0	33.0	40.0
20	37.61161559559095	40.0	37.0	40.0	33.0	40.0
21	37.61193385906375	40.0	37.0	40.0	33.0	40.0
22	37.7269775947621	40.0	37.0	40.0	33.0	40.0
23	37.68109744733973	40.0	37.0	40.0	33.0	40.0
24	37.58325525666587	39.0	37.0	40.0	33.0	40.0
25	37.56606647621916	39.0	36.0	40.0	33.0	40.0
26	37.36996171477636	39.0	36.0	40.0	33.0	40.0
27	37.26358682933844	39.0	36.0	40.0	33.0	40.0
28	37.037435953730956	39.0	35.0	40.0	32.0	40.0
29	37.16244201690541	39.0	36.0	40.0	33.0	40.0
30	37.321910738158515	40.0	36.0	40.0	33.0	40.0
31	37.39804038201829	40.0	36.0	40.0	33.0	40.0
32	37.39867265410462	40.0	36.0	40.0	33.0	40.0
33	37.260585451615015	40.0	35.0	40.0	33.0	40.0
34	36.87283672317971	40.0	35.0	40.0	32.0	40.0
35	36.81777799335731	39.0	35.0	40.0	32.0	40.0
36	36.76184871932991	39.0	35.0	40.0	32.0	40.0
37	36.66434691739701	39.0	35.0	40.0	32.0	40.0
38	36.56418923231276	39.0	35.0	40.0	32.0	40.0
39	36.42433711420127	39.0	35.0	40.0	31.0	40.0
40	36.18143826156662	39.0	35.0	40.0	31.0	40.0
41	36.011462590851885	38.0	35.0	40.0	31.0	40.0
42	35.877725556471766	38.0	35.0	40.0	30.0	40.0
43	35.71550138836058	38.0	35.0	40.0	30.0	40.0
44	35.330718296830895	37.0	34.0	40.0	29.0	40.0
45	35.29064177744193	37.0	34.0	40.0	29.0	40.0
46	35.072490037247036	37.0	34.0	40.0	29.0	40.0
47	35.014595018240584	36.0	34.0	40.0	29.0	40.0
48	34.8735413278735	36.0	34.0	40.0	29.0	40.0
49	34.74656101000147	35.0	34.0	40.0	28.0	40.0
50	33.98766856688681	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	4.0
15	13.0
16	37.0
17	82.0
18	175.0
19	320.0
20	517.0
21	841.0
22	1383.0
23	2218.0
24	3316.0
25	4591.0
26	6312.0
27	8431.0
28	11313.0
29	14608.0
30	19113.0
31	25109.0
32	35152.0
33	53101.0
34	89807.0
35	96645.0
36	149701.0
37	246568.0
38	333598.0
39	72167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.20757501104136	0.6028284602430206	0.1325814146045491	0.05701511411107055
8	99.20714952511516	0.5855537316392186	0.15351532217368846	0.05378142107193521
9	97.95349779215353	1.2999446017324083	0.3753636840954212	0.3711939220186414
10	63.09760562049889	27.062521752967978	4.09045150013573	5.749421126397402
11	36.29880004459093	26.14704623415171	19.02764552256905	18.526508198688312
12	30.73659272572241	17.397353647733393	26.35383239428589	25.5122212322583
13	24.321881805115535	17.94929399120265	29.37716519150696	28.351659012174856
14	25.417763356641455	18.137699159324907	30.09972539138323	26.34481209265041
15	25.765725747089462	18.342102598272355	29.176591125895328	26.715580528742848
16	30.762972853146938	19.268470556799393	24.656569034666038	25.31198755538763
17	32.364672073741815	19.848322777027505	22.264402060373047	25.522603088857633
18	30.138274416297133	19.69412667737189	23.074442166676455	27.09315673965452
19	31.395244939483135	20.046258829896686	21.84691526958363	26.711580961036553
20	30.181673980769734	21.639873817893726	22.10024959004431	26.078202611292227
21	31.713253120726527	20.35567219543079	23.584855083748398	24.346219600094287
22	31.06923762282715	19.152568190501963	24.249464100475947	25.52873008619494
23	31.33354948018384	20.153991866411033	22.84672209897313	25.66573655443199
24	31.494808646214413	19.77241608779306	22.241170528802417	26.491604737190105
25	31.371758116356784	19.58664893241326	21.414196082636174	27.627396868593777
26	29.028862412317984	19.633197092739763	22.765879772994747	28.5720607219475
27	28.790164807718654	18.577055926721112	23.02602186827466	29.60675739728557
28	29.852262776704137	20.889571935629085	21.434789601464352	27.82337568620243
29	31.372268699468226	21.24332093467344	21.15209675209573	26.2323136137626
30	29.98144030389907	20.05825753301558	22.425320837662653	27.5349813254227
31	31.31814688965533	20.937992234030876	21.028705833497146	26.715155042816647
32	34.210259827235696	19.905593182694297	21.616727383508337	24.267419606561674
33	33.90884559711419	19.826878286346922	22.153775719560524	24.110500396978367
34	30.954101131196882	22.558753224119606	23.928392420563902	22.558753224119606
35	30.25400658822408	24.147772964113667	24.047783771456192	21.55043667620606
36	30.845006539718682	24.27329131234326	21.250469098233637	23.631233049704413
37	30.73233786646039	24.87943856281066	20.421707611177347	23.966515959551607
38	32.23098439572914	23.90805419329145	20.339844118976078	23.521117292003332
39	31.532591796461144	22.529309598026426	20.657171522737542	25.280927082774884
40	30.448623850868884	22.527267265580655	20.654278218439366	26.36983066511109
41	28.300685798215852	22.453402908791986	21.400155047071507	27.845756245920654
42	27.970764011038806	20.569691616310408	22.882462916774102	28.577081455876684
43	27.018356313828207	19.56273662336071	24.758600559769285	28.6603065030418
44	28.063775234506565	19.20039280860707	24.105990246160626	28.629841710725735
45	28.3995687274652	19.88108519334506	23.632084021556818	28.087262057632923
46	28.155254708640005	21.429853964720408	22.518672449871378	27.896218876768213
47	28.604482749524095	21.26970106209797	23.130351017379397	26.99546517099854
48	28.256265067520363	21.916694961480758	23.608086615319024	26.21895335567985
49	29.089111219468194	20.129909362988	24.02233971306931	26.758639704474497
50	26.60546477104177	21.42406735612406	24.621338799976513	27.349129072857657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	6.0
6	12.0
7	41.0
8	70.0
9	88.5
10	107.0
11	656.5
12	1206.0
13	1325.0
14	1444.0
15	1684.0
16	1924.0
17	1879.5
18	1835.0
19	1636.5
20	1438.0
21	1823.0
22	2208.0
23	2479.5
24	2751.0
25	3580.5
26	4410.0
27	5026.0
28	5642.0
29	5756.5
30	5871.0
31	6540.5
32	7210.0
33	8634.5
34	10059.0
35	10699.5
36	11340.0
37	12723.5
38	14107.0
39	15057.5
40	16008.0
41	18833.0
42	21658.0
43	23172.5
44	24687.0
45	26541.5
46	28396.0
47	28945.0
48	29494.0
49	36166.0
50	42838.0
51	47550.5
52	52263.0
53	56388.0
54	60513.0
55	68688.5
56	76864.0
57	90319.5
58	103775.0
59	110205.0
60	116635.0
61	120111.0
62	123587.0
63	119721.5
64	115856.0
65	101580.5
66	87305.0
67	77166.5
68	67028.0
69	55192.5
70	43357.0
71	37808.0
72	32259.0
73	27702.0
74	23145.0
75	22964.5
76	22784.0
77	16175.0
78	9566.0
79	6493.0
80	3420.0
81	2353.5
82	1287.0
83	887.0
84	487.0
85	348.0
86	209.0
87	126.5
88	44.0
89	35.5
90	27.0
91	14.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1175127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.398053462461817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.11540580278113	6.4015222535181895
2	9.54392640740027	1.7938866123709776
3	3.7604187295475917	1.06021848784593
4	1.8531581529786032	0.6966431758435963
5	0.9870350426689908	0.4638104050163228
6	0.6467541774981063	0.36469382023186336
7	0.4633990754304377	0.30485344997454406
8	0.3712664137028634	0.27913452838367814
9	0.33386609242637333	0.2823922247333647
>10	7.830345665786946	21.58508759494621
>50	4.176974930361721	27.123970137498333
>100	1.81893164689698	29.403168566263787
>500	0.06659077744407199	4.184874418874055
>1k	0.028278275352962078	4.057566449765626
>5k	0.003648809722962849	1.9981778747335137
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	6139	0.522411620190839	No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5918	0.5036051422527097	No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5802	0.49373386876482284	No Hit
AGTCGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5449	0.4636945623749603	No Hit
AGTCGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2917	0.2482284893462579	No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2781	0.236655272153563	No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2492	0.21206218561908627	No Hit
AGTCGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2100	0.178704089004848	No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2099	0.17861899181960758	No Hit
AGTCGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2072	0.1763213678181167	No Hit
AGTCGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2033	0.1730025775937409	No Hit
AGTCGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1758	0.14960085165262987	No Hit
AGTCGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1754	0.14926046291166825	No Hit
AGTCGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1720	0.14636715861349453	No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1631	0.13879350912709862	No Hit
AGTCGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1603	0.1364107879403673	No Hit
AGTCGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1477	0.12568854260007642	No Hit
AGTCGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	1370	0.11658314377935321	No Hit
AGTCGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	1340	0.1140302282221411	No Hit
AGTCGTGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	1294	0.11011575770108252	No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1284	0.10926478584867849	No Hit
AGTCGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1276	0.10858400836675525	No Hit
AGTCGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1272	0.10824361962579364	No Hit
AGTCGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1271	0.10815852244055323	No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1179	0.10032958139843608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.658746671636343E-4	0.0	0.0	0.0	0.0
8	0.007743843856876746	0.0	0.0	0.0	0.0
9	0.009871273487886842	0.0	0.0	0.0	0.0
10	0.04084664891539382	0.0	0.0	0.0	0.0
11	0.06441856922698568	0.0	0.0	0.0	0.0
12	0.07343887086246849	0.0	0.0	0.0	0.0
13	0.0850120880551634	0.0	0.0	0.0	0.0
14	0.09667040243309873	0.0	0.0	0.0	0.0
15	0.10654167592098557	0.0	0.0	0.0	0.0
16	0.12236975237570066	0.0	0.0	0.0	0.0
17	0.13590020482892487	0.0	0.0	0.0	0.0
18	0.14866478261498545	0.0	0.0	0.0	0.0
19	0.16296110973537328	0.0	0.0	0.0	0.0
20	0.17785311715244395	0.0	0.0	0.0	0.0
21	0.19410667953336108	0.0	0.0	0.0	0.0
22	0.20891358976519134	0.0	0.0	0.0	0.0
23	0.22499695777562764	0.0	0.0	0.0	0.0
24	0.24073993704510235	0.0	0.0	0.0	0.0
25	0.25665311068505786	0.0	0.0	0.0	0.0
26	0.27401293647410024	0.0	0.0	0.0	0.0
27	0.2882241664092477	0.0	0.0	0.0	0.0
28	0.30711574153261734	0.0	0.0	0.0	0.0
29	0.3203058052448799	0.0	0.0	0.0	0.0
30	0.33281509147521926	0.0	0.0	0.0	0.0
31	0.34498398896459703	0.0	0.0	0.0	0.0
32	0.3596207048259465	0.0	0.0	0.0	0.0
33	0.3686410064614293	0.0	0.0	0.0	0.0
34	0.38336281950801915	0.0	0.0	0.0	0.0
35	0.39927599314797463	0.0	0.0	0.0	0.0
36	0.4131468343421605	0.0	0.0	0.0	0.0
37	0.42599650931346145	0.0	0.0	0.0	0.0
38	0.4386759899142816	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAGA	20	7.8565546E-4	44.0	26
TGATACG	20	7.8565546E-4	44.0	24
CGTATAG	20	7.8565546E-4	44.0	41
ATCTAGT	25	4.4429016E-5	44.0	30
CGACGTA	25	4.4429016E-5	44.0	29
TAGACTA	25	4.4429016E-5	44.0	20
GCGAATA	30	2.527766E-6	44.0	31
GCGCGTA	25	4.4429016E-5	44.0	40
CACGATA	20	7.8565546E-4	44.0	32
TAAGTCG	25	4.4429016E-5	44.0	43
TATGCGC	50	2.7284841E-11	44.0	26
TACATAC	25	4.4429016E-5	44.0	33
CCGTATA	20	7.8565546E-4	44.0	40
ACGATAT	20	7.8565546E-4	44.0	33
TCTAACG	20	7.8565546E-4	44.0	33
CGAATTT	25	4.4429016E-5	44.0	42
CGAATAC	30	2.527766E-6	44.0	32
CCGCGTA	20	7.8565546E-4	44.0	44
ATACGCC	20	7.8565546E-4	44.0	26
GCGCTTA	40	8.312782E-9	44.0	16
>>END_MODULE