FastQCFastQC Report
Fri 17 Jun 2016
SRR1527644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468551
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT76040.5177893038784489No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT75560.5145207759212993No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC73210.49851860779775437No Hit
AGTCGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG70570.4805417040334316No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC36820.25072333204635044No Hit
AGTCGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC36000.24513959678621988No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33660.2292055229951156No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC27460.18698703688193327No Hit
AGTCGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC27300.1858975275628834No Hit
AGTCGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT26570.1809266412947184No Hit
AGTCGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG25330.17248294407208195No Hit
AGTCGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT23510.1600897755678897No Hit
AGTCGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG22260.1515779840128126No Hit
AGTCGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG21940.1493989653747129No Hit
AGTCGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC21320.14517711676339465No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA19980.13605247621635203No Hit
AGTCGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT18400.12529357169073463No Hit
AGTCGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT17270.11759891212494492No Hit
AGTCGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG16970.11555608215172643No Hit
AGTCGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC16330.11119804487552698No Hit
AGTCGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG16230.11051710155112081No Hit
AGTCGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA16050.10929140356718971No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA15610.10629525293980256No Hit
AGTCGTGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG15590.10615906427492133No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT15490.10547812095051516No Hit
AGTCGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA15110.10289053631777174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA351.0198346E-746.00000411
GACGTTA351.0198346E-746.00000410
CGCTATA351.0198346E-746.00000413
AGCGTAC253.4176228E-546.045
CGTATAG453.110472E-1046.041
CGAAATT206.3120294E-446.014
GCGAATA301.8617593E-646.031
CGCATTC206.3120294E-446.043
CGCGATT453.110472E-1046.019
TAACGCA405.6134013E-946.022
TCGATAG301.8617593E-646.044
TCGGCAT206.3120294E-446.045
CCGTATA453.110472E-1046.040
AGGCATA206.3120294E-446.019
CGAATAC301.8617593E-646.032
GCGATTA301.8617593E-646.030
AACGAAA551.8189894E-1246.013
ACTAACG253.4176228E-546.017
TCCGTAC206.3120294E-446.040
CGGTATA253.4176228E-546.043
Produced by FastQC (version 0.11.5)