##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527644_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1468551
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624136308510906	31.0	31.0	33.0	30.0	34.0
2	32.317553152733545	33.0	31.0	34.0	31.0	34.0
3	30.87502102412514	31.0	31.0	31.0	30.0	33.0
4	35.75679292036845	37.0	35.0	37.0	35.0	37.0
5	36.03745733038893	37.0	35.0	37.0	35.0	37.0
6	35.220144210177246	35.0	35.0	37.0	33.0	37.0
7	36.27530947171736	37.0	35.0	37.0	35.0	37.0
8	36.61761899995302	37.0	37.0	37.0	35.0	37.0
9	38.699128596827755	39.0	39.0	39.0	38.0	39.0
10	38.09865574978329	39.0	38.0	39.0	37.0	39.0
11	37.66418054258926	39.0	37.0	39.0	35.0	39.0
12	37.1039739171469	39.0	37.0	39.0	34.0	39.0
13	36.97514148299923	39.0	37.0	39.0	33.0	39.0
14	37.83872946870759	40.0	37.0	40.0	33.0	40.0
15	37.89307487448512	40.0	37.0	40.0	33.0	40.0
16	37.913633915335595	40.0	37.0	40.0	34.0	40.0
17	37.82700975315124	40.0	37.0	40.0	33.0	40.0
18	37.76917451283612	40.0	37.0	40.0	33.0	40.0
19	37.752657551559324	40.0	37.0	40.0	33.0	40.0
20	37.66901319736257	40.0	37.0	40.0	33.0	40.0
21	37.652236796679176	40.0	37.0	40.0	33.0	40.0
22	37.621971589682616	40.0	37.0	40.0	33.0	40.0
23	37.57350681045466	40.0	37.0	40.0	33.0	40.0
24	37.4608263519619	40.0	36.0	40.0	33.0	40.0
25	37.43740462537563	39.0	36.0	40.0	33.0	40.0
26	37.23461357487755	39.0	36.0	40.0	33.0	40.0
27	37.15186261832241	39.0	35.0	40.0	33.0	40.0
28	36.97883151487419	39.0	35.0	40.0	32.0	40.0
29	36.872822939073956	39.0	35.0	40.0	32.0	40.0
30	36.701257906603175	39.0	35.0	40.0	31.0	40.0
31	36.596675226124255	38.0	35.0	40.0	31.0	40.0
32	36.488714385812955	38.0	35.0	40.0	31.0	40.0
33	36.27020920621756	38.0	35.0	40.0	31.0	40.0
34	36.0907016508109	38.0	35.0	40.0	30.0	40.0
35	35.933695867559244	38.0	35.0	40.0	30.0	40.0
36	35.877983127586305	38.0	34.0	40.0	30.0	40.0
37	35.780327683546574	38.0	34.0	40.0	30.0	40.0
38	35.67449479112404	38.0	34.0	40.0	30.0	40.0
39	35.525214990831095	38.0	34.0	40.0	29.0	40.0
40	35.26287340378373	37.0	34.0	40.0	28.0	40.0
41	35.090790173443075	37.0	34.0	40.0	28.0	40.0
42	34.944225975127864	37.0	33.0	40.0	28.0	40.0
43	34.764781066507055	36.0	33.0	40.0	27.0	40.0
44	34.619140908283065	36.0	33.0	39.0	27.0	40.0
45	34.4435950811378	36.0	33.0	39.0	27.0	40.0
46	34.26084283079035	35.0	33.0	39.0	26.0	40.0
47	34.076555053246366	35.0	33.0	39.0	26.0	40.0
48	33.79347738008418	35.0	33.0	39.0	25.0	40.0
49	34.1979481815749	35.0	33.0	39.0	27.0	40.0
50	34.322071892634305	35.0	33.0	39.0	27.0	40.0
51	34.30210527247607	35.0	33.0	39.0	27.0	40.0
52	33.755909055933365	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	12.0
16	47.0
17	105.0
18	188.0
19	466.0
20	729.0
21	1363.0
22	2341.0
23	3598.0
24	5408.0
25	7426.0
26	10138.0
27	13684.0
28	17366.0
29	22389.0
30	28589.0
31	37758.0
32	50639.0
33	73309.0
34	127422.0
35	133106.0
36	196833.0
37	279129.0
38	402276.0
39	54225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.28868660332532	0.582070353702391	0.10466098896122777	0.024582054011062605
8	99.27431869918036	0.5475465271549984	0.12876638264520604	0.04936839101944707
9	97.96057474340353	1.3059812018785864	0.3408121338652863	0.3926319208525955
10	63.23498468898935	26.943974026097834	4.049025195583946	5.7720160893288694
11	36.34017477091364	26.222037913562417	18.854980181144544	18.582807134379397
12	30.803765071829307	17.271514574570443	26.32860554383198	25.596114809768267
13	24.45138098710906	17.830569043907907	29.286759533717248	28.431290435265783
14	25.516512535145186	18.1060106186302	30.051731264355137	26.325745581869477
15	25.85112808475838	18.16559315951574	29.245153896596033	26.738124859129847
16	30.802266996515616	19.03325114347408	24.706870922426255	25.45761093758405
17	32.50687242050157	19.67510832105933	22.303753836264452	25.51426542217465
18	30.34038313957091	19.502693471319688	23.003831668086434	27.153091721022964
19	31.318081564753285	19.978264289084954	21.790526852659525	26.913127293502235
20	30.23136411333348	21.621857191204118	22.006658263826044	26.140120431636355
21	31.769887460496776	20.2791050498076	23.541640705702424	24.409366783993203
22	31.227924668601908	19.009690504449626	24.252000781722938	25.510384045225532
23	31.41688644112462	20.000939701787683	22.756921618656758	25.825252238430945
24	31.427441062652917	19.668026510485507	22.304775251251062	26.599757175610517
25	31.332040902903614	19.401777670642694	21.510182486001508	27.755998940452187
26	29.054489765762305	19.387001200503082	22.81207802793366	28.746431005800954
27	28.73362927130212	18.477329013428882	23.014931044274253	29.77411067099474
28	29.79031712211561	20.853957404271284	21.48280856436038	27.872916909252726
29	31.287643398152326	21.037335441533866	21.21853446015835	26.45648670015546
30	30.00467808063867	20.00223349410405	22.377772375627405	27.61531604962987
31	31.44712032472825	20.78388833618989	20.934853471210737	26.83413786787112
32	34.217878711736944	19.85726066033798	21.5403482752727	24.384512352652376
33	33.99691260296714	19.678104471686716	22.172263680321624	24.152719245024517
34	30.833794672435616	22.27140902835516	24.097154269752973	22.79764202945625
35	30.225780378073352	24.06487755617612	24.131473813303046	21.57786825244748
36	30.902910419862845	24.17627988404897	21.26511098354773	23.655698712540456
37	30.897462873267596	24.73261058008881	20.33562334573331	24.034303200910283
38	32.220195280926575	23.846703315036386	20.34311372230178	23.589987681735263
39	31.54803612540525	22.453016612974285	20.64252450204317	25.356422759577296
40	30.386687285630533	22.541607339479526	20.666357518397387	26.405347856492554
41	28.365511310128145	22.364153509139282	21.45448132206508	27.815853858667488
42	27.99814238661102	20.47903000985325	22.833187270990248	28.68964033254548
43	26.970530815749676	19.418120310428442	24.87622152720607	28.735127346615812
44	27.78861612569124	19.092765590027177	24.32424886844243	28.79436941583915
45	28.342291142765895	19.812863155586697	23.658967240497606	28.185878461149798
46	27.98159546382795	21.266609058861423	22.47541964834725	28.276375828963378
47	28.607654756286983	21.093377077132494	23.27300856422419	27.025959602356338
48	28.386416270187414	21.733327613409408	23.602517038904335	26.27773907749884
49	29.050131728486107	19.993449325219213	24.142777472488184	26.81364147380649
50	26.550865444918152	21.402933912407537	24.702444790817616	27.343755851856695
51	26.472012207951924	21.120070055449215	24.357887468668096	28.05003026793077
52	27.253326578375553	20.856953554898674	24.17205803543765	27.71766183128812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	8.0
6	16.0
7	48.5
8	81.0
9	99.5
10	118.0
11	700.0
12	1282.0
13	1444.5
14	1896.0
15	2185.0
16	2019.0
17	1853.0
18	1775.0
19	1697.0
20	1918.5
21	2140.0
22	2432.5
23	2725.0
24	3153.5
25	3582.0
26	5005.0
27	6428.0
28	6448.0
29	6468.0
30	6818.5
31	7169.0
32	8517.5
33	9866.0
34	10183.5
35	10501.0
36	13676.0
37	16851.0
38	16598.0
39	19029.0
40	21713.0
41	24028.5
42	26344.0
43	28255.5
44	30167.0
45	32274.0
46	34381.0
47	35844.5
48	37308.0
49	43853.0
50	50398.0
51	55310.5
52	60223.0
53	68625.0
54	77027.0
55	84018.5
56	91010.0
57	101931.5
58	112853.0
59	132847.0
60	152841.0
61	147332.0
62	141823.0
63	143811.0
64	130681.5
65	115564.0
66	101596.5
67	87629.0
68	74531.0
69	61433.0
70	50847.5
71	40262.0
72	37265.0
73	34268.0
74	33172.5
75	32077.0
76	23935.5
77	15794.0
78	10634.0
79	5474.0
80	3730.5
81	1987.0
82	1440.5
83	894.0
84	574.5
85	255.0
86	155.5
87	56.0
88	42.0
89	26.5
90	25.0
91	13.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1468551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.52760699774399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.51036693517085	5.842294844943714
2	9.444354942069628	1.6107549458634198
3	4.104651744219514	1.050085708119252
4	2.1206862337127323	0.7233751506651217
5	1.2042522859397105	0.5134695110314335
6	0.729931290512128	0.3734740308506114
7	0.5005488425571071	0.2987938668751849
8	0.3719808754949802	0.2537685373518343
9	0.29294648362480197	0.22483192353510206
>10	5.684854784789815	14.047106917609083
>50	4.525852399526043	27.53040281197939
>100	2.3882452168222086	35.571439829839925
>500	0.08221566087322442	4.799491417476608
>1k	0.035919463488301925	5.150110908207313
>5k	0.0031928411989601713	2.0105995956520215
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7604	0.5177893038784489	No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7556	0.5145207759212993	No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7321	0.49851860779775437	No Hit
AGTCGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7057	0.4805417040334316	No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3682	0.25072333204635044	No Hit
AGTCGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3600	0.24513959678621988	No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3366	0.2292055229951156	No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2746	0.18698703688193327	No Hit
AGTCGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2730	0.1858975275628834	No Hit
AGTCGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2657	0.1809266412947184	No Hit
AGTCGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2533	0.17248294407208195	No Hit
AGTCGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2351	0.1600897755678897	No Hit
AGTCGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2226	0.1515779840128126	No Hit
AGTCGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2194	0.1493989653747129	No Hit
AGTCGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2132	0.14517711676339465	No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1998	0.13605247621635203	No Hit
AGTCGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1840	0.12529357169073463	No Hit
AGTCGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1727	0.11759891212494492	No Hit
AGTCGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1697	0.11555608215172643	No Hit
AGTCGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1633	0.11119804487552698	No Hit
AGTCGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1623	0.11051710155112081	No Hit
AGTCGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1605	0.10929140356718971	No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1561	0.10629525293980256	No Hit
AGTCGTGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	1559	0.10615906427492133	No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1549	0.10547812095051516	No Hit
AGTCGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	1511	0.10289053631777174	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.809433244061664E-4	0.0	0.0	0.0	0.0
8	0.005788018257452414	0.0	0.0	0.0	0.0
9	0.007830848230670913	0.0	0.0	0.0	0.0
10	0.032821468236377216	0.0	0.0	0.0	0.0
11	0.05433927728761207	0.0	0.0	0.0	0.0
12	0.061625370858758056	0.0	0.0	0.0	0.0
13	0.07306521870878165	0.0	0.0	0.0	0.0
14	0.08089606693945256	0.0	0.0	0.0	0.0
15	0.0912464054704263	0.0	0.0	0.0	0.0
16	0.10384385697194037	0.0	0.0	0.0	0.0
17	0.11514751615708273	0.0	0.0	0.0	0.0
18	0.1277449676585968	0.0	0.0	0.0	0.0
19	0.1411595511493983	0.0	0.0	0.0	0.0
20	0.15273558766430312	0.0	0.0	0.0	0.0
21	0.16431162417920794	0.0	0.0	0.0	0.0
22	0.17752192467268757	0.0	0.0	0.0	0.0
23	0.19168554582033584	0.0	0.0	0.0	0.0
24	0.2059172613004247	0.0	0.0	0.0	0.0
25	0.21803805247485447	0.0	0.0	0.0	0.0
26	0.23158882463053718	0.0	0.0	0.0	0.0
27	0.24350533280764508	0.0	0.0	0.0	0.0
28	0.25726038796064965	0.0	0.0	0.0	0.0
29	0.26788310382138586	0.0	0.0	0.0	0.0
30	0.278505819682122	0.0	0.0	0.0	0.0
31	0.287221894234521	0.0	0.0	0.0	0.0
32	0.30056838339288183	0.0	0.0	0.0	0.0
33	0.31132728791849923	0.0	0.0	0.0	0.0
34	0.3229033244334041	0.0	0.0	0.0	0.0
35	0.3384288322298647	0.0	0.0	0.0	0.0
36	0.3508220007340569	0.0	0.0	0.0	0.0
37	0.3636237352328928	0.0	0.0	0.0	0.0
38	0.37424645109362903	0.0	0.0	0.0	0.0
39	0.38384775196775595	0.0	0.0	0.0	0.0
40	0.39481093949069523	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAA	35	1.0198346E-7	46.000004	11
GACGTTA	35	1.0198346E-7	46.000004	10
CGCTATA	35	1.0198346E-7	46.000004	13
AGCGTAC	25	3.4176228E-5	46.0	45
CGTATAG	45	3.110472E-10	46.0	41
CGAAATT	20	6.3120294E-4	46.0	14
GCGAATA	30	1.8617593E-6	46.0	31
CGCATTC	20	6.3120294E-4	46.0	43
CGCGATT	45	3.110472E-10	46.0	19
TAACGCA	40	5.6134013E-9	46.0	22
TCGATAG	30	1.8617593E-6	46.0	44
TCGGCAT	20	6.3120294E-4	46.0	45
CCGTATA	45	3.110472E-10	46.0	40
AGGCATA	20	6.3120294E-4	46.0	19
CGAATAC	30	1.8617593E-6	46.0	32
GCGATTA	30	1.8617593E-6	46.0	30
AACGAAA	55	1.8189894E-12	46.0	13
ACTAACG	25	3.4176228E-5	46.0	17
TCCGTAC	20	6.3120294E-4	46.0	40
CGGTATA	25	3.4176228E-5	46.0	43
>>END_MODULE