FastQCFastQC Report
Fri 17 Jun 2016
SRR1527643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476725
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT72830.49318593509285746No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT72240.48919060759450816No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC70190.4753085374731247No Hit
AGTCGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG67600.45776972692952306No Hit
AGTCGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC36290.2457464998561005No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC35740.24202204201865615No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG30010.20321996309400872No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC26330.17829995429074472No Hit
AGTCGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC25030.1694966903113308No Hit
AGTCGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT24460.16563679764343395No Hit
AGTCGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG24040.16279266620393099No Hit
AGTCGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT21710.14701450845621222No Hit
AGTCGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG21100.1428837461274103No Hit
AGTCGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG21010.14227428939037398No Hit
AGTCGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC20590.13943015795087102No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA19590.13265841642824494No Hit
AGTCGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT17310.11721884575665747No Hit
AGTCGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT17290.11708341092620494No Hit
AGTCGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG17160.11620308452826357No Hit
AGTCGTGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG15520.10509742843115678No Hit
AGTCGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC14790.10015405711963975No Hit
AGTCGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG14780.10008633970441348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG453.110472E-1046.041
GCGAATA750.046.031
ATTACTC253.4176326E-546.030
TCGGCAT206.312042E-446.045
CCGTATA453.110472E-1046.040
CTACGTA206.312042E-446.017
TTCATAC206.312042E-446.038
CGCGTAT453.110472E-1046.041
CGGTAAT253.4176326E-546.035
TATCGTC206.312042E-446.023
TACCGTT301.8617666E-646.027
CGAATAC700.045.99999632
GCGTATA351.0198346E-745.99999642
AGTCGTG1522850.044.3084341
CGTGGGG954850.044.2175184
TCGTGGG1490450.044.1497543
CGTGGGA400000.044.0450024
GTCGTGG1526350.043.929572
GTGGGGG364100.043.7764325
GTGGGGA281700.043.2648245
Produced by FastQC (version 0.11.5)