FastQCFastQC Report
Fri 17 Jun 2016
SRR1527642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189536
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC131531.1057252575794259No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT57060.47968283431522885No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG55400.46572781319775103No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC51730.4348754472332069No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC42850.3602244908939284No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT36110.30356374250127777No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC32060.26951685363032307No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC31520.2649772684475291No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG28890.24286780727947704No Hit
AGGGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA21310.17914548193581362No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA20720.17418556479164984No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG20570.17292456890754043No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA19110.16065087563554192No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG18800.1580448174750491No Hit
AGGGTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC17270.14518265945713285No Hit
AGGGTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA16910.14215626933527023No Hit
AGGGTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA16160.13585128991472306No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAAGAACCGACTGC16140.13568315713017512No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT15600.13114357194738116No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC15440.12979850967099776No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15340.12895784574825817No Hit
AGGGTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC14620.12290506550453285No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG14340.12055120652086193No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGGAAAAGCTCCGACTGCT13750.11559128937669814No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT13430.11290116482393134No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG12880.10827751324886342No Hit
AGGGTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC12800.10760498211067172No Hit
AGGGTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT12700.1067643181879321No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT12490.10499892395017889No Hit
AGGGTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG12370.10399012724289133No Hit
AGGGTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG11960.10054340515965889No Hit
AGGGTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC11940.10037527237511097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC207.856592E-444.00000411
TCCGATA408.312782E-944.00000441
CCGATAT408.312782E-944.00000442
CGCATAC207.856592E-444.00000439
AAGCGTA207.856592E-444.00000410
TATGCGA207.856592E-444.00000418
TGCGATA207.856592E-444.00000420
CGTAAGG207.856592E-444.00000416
ATGCGAT207.856592E-444.00000419
CCGAATC207.856592E-444.00000419
ATACGGG207.856592E-444.00000424
TCGACTA207.856592E-444.00000414
CGATAGC207.856592E-444.00000436
GTACGTT207.856592E-444.00000435
AATCGCT207.856592E-444.00000425
TAGCGTT454.802132E-1044.039
TCGCTTA254.4429355E-544.042
TGTTACG254.4429355E-544.036
TATACGC950.044.019
GCGTAAC254.4429355E-544.09
Produced by FastQC (version 0.11.5)