FastQCFastQC Report
Fri 17 Jun 2016
SRR1527641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518764
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC170051.119660460743078No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG75780.4989583635113816No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT72210.4754524073522943No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT64840.42692610570174166No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC55630.36628468939216363No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT47930.3155855682647205No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC40990.2698905162355705No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA40500.2666642085274605No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC32390.21326552380751718No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT32030.21089517528727308No Hit
AGGGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG26820.17659096475818495No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG26350.17349634307897738No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG26030.1713893666165382No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG25310.16664866957605No Hit
AGGGTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA23220.15288747955574403No Hit
AGGGTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG21410.14096989394007234No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAAGAACCGACTGCTC20560.13537323771171822No Hit
AGGGTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG20430.13451727852385229No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT20330.13385884837934003No Hit
AGGGTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT19330.12727454693421755No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG19150.1260893726740955No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19090.12569431458738817No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT18560.12220463482147326No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGGAAAAGCTCCGACTGCTCT16950.11160390949482606No Hit
AGGGTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC16890.11120885140811872No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT16250.10699489848324031No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC16200.1066656834109842No Hit
AGGGTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG15420.10152992828378866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC351.0198528E-746.00000416
TCGTTAA206.312099E-446.045
CAACGAC301.8618011E-646.037
CGCATAC206.312099E-446.039
CGTTGAT206.312099E-446.014
TAGCGAT253.417678E-546.031
CACGCTA206.312099E-446.027
TCTAGCG253.417678E-546.031
CGAATCT206.312099E-446.046
AACGACT301.8618011E-646.038
CTCGCTA405.6134013E-946.041
ACGCTAA301.8618011E-646.010
ACGTAGG206.312099E-446.040
ATACGGA206.312099E-446.024
CGCGTAT301.8618011E-646.034
TAATGCG206.312099E-446.040
ATCGCGT206.312099E-446.031
TATACGC1450.045.99999619
ATACGCA1500.044.46666320
AGGGTCG1559200.044.4378551
Produced by FastQC (version 0.11.5)