##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527641_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1518764
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49062461317229	31.0	31.0	33.0	30.0	34.0
2	32.16683105472608	31.0	31.0	34.0	31.0	34.0
3	32.76664313876284	34.0	31.0	34.0	31.0	34.0
4	36.34053282801015	37.0	37.0	37.0	35.0	37.0
5	33.520874869301615	35.0	33.0	35.0	30.0	35.0
6	35.17141372853189	35.0	35.0	37.0	33.0	37.0
7	36.075889341596195	37.0	35.0	37.0	35.0	37.0
8	36.540876001801465	37.0	37.0	37.0	35.0	37.0
9	38.62981477043174	39.0	39.0	39.0	37.0	39.0
10	37.97045821470617	39.0	38.0	39.0	35.0	39.0
11	37.524675986525885	39.0	37.0	39.0	35.0	39.0
12	36.859064344427445	39.0	35.0	39.0	33.0	39.0
13	36.736313871016165	39.0	35.0	39.0	33.0	39.0
14	37.51587738450477	40.0	37.0	40.0	33.0	40.0
15	37.55281597404205	40.0	37.0	40.0	33.0	40.0
16	37.56990618687301	40.0	36.0	40.0	33.0	40.0
17	37.43381657716406	40.0	36.0	40.0	33.0	40.0
18	37.37528806318822	39.0	36.0	40.0	33.0	40.0
19	37.354501423525974	39.0	36.0	40.0	33.0	40.0
20	37.27398924388516	39.0	36.0	40.0	32.0	40.0
21	37.24140880347441	39.0	36.0	40.0	32.0	40.0
22	37.20293475484012	39.0	35.0	40.0	32.0	40.0
23	37.148469413286065	39.0	35.0	40.0	32.0	40.0
24	37.06304073575618	39.0	35.0	40.0	32.0	40.0
25	36.98323241793985	39.0	35.0	40.0	32.0	40.0
26	36.804882127835526	39.0	35.0	40.0	32.0	40.0
27	36.66231949137588	38.0	35.0	40.0	31.0	40.0
28	36.49693434924715	38.0	35.0	40.0	31.0	40.0
29	36.3246231804283	38.0	35.0	40.0	31.0	40.0
30	36.18203157304229	38.0	35.0	40.0	31.0	40.0
31	36.007126189454056	38.0	35.0	40.0	30.0	40.0
32	35.86726048286633	38.0	34.0	40.0	30.0	40.0
33	35.61625703532609	37.0	34.0	40.0	30.0	40.0
34	35.234503846548904	37.0	34.0	40.0	28.0	40.0
35	35.07050009086336	37.0	33.0	40.0	28.0	40.0
36	35.07129086546692	37.0	33.0	40.0	28.0	40.0
37	34.90676760839735	36.0	33.0	39.0	28.0	40.0
38	34.79533028172909	36.0	33.0	39.0	28.0	40.0
39	34.61538198166404	36.0	33.0	39.0	27.0	40.0
40	34.30197713403794	35.0	33.0	39.0	26.0	40.0
41	34.115472186593834	35.0	33.0	39.0	26.0	40.0
42	34.01303230785033	35.0	33.0	39.0	26.0	40.0
43	33.846683223990034	35.0	33.0	38.0	26.0	40.0
44	33.69420594641432	35.0	33.0	38.0	26.0	40.0
45	33.53018638840531	35.0	32.0	38.0	25.0	40.0
46	33.30238206857682	35.0	32.0	38.0	24.0	40.0
47	33.11885980968735	35.0	32.0	37.0	24.0	40.0
48	32.895124588151944	35.0	31.0	37.0	23.0	40.0
49	33.346593019060236	35.0	32.0	37.0	26.0	40.0
50	33.54893189461957	35.0	33.0	37.0	26.0	40.0
51	33.5776275971777	35.0	33.0	37.0	27.0	40.0
52	33.11633670537358	35.0	32.0	36.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	15.0
15	33.0
16	69.0
17	157.0
18	275.0
19	623.0
20	1100.0
21	1951.0
22	3040.0
23	4716.0
24	6867.0
25	9518.0
26	12601.0
27	17573.0
28	22716.0
29	28855.0
30	37400.0
31	47665.0
32	62770.0
33	89700.0
34	159943.0
35	170163.0
36	234645.0
37	275379.0
38	296646.0
39	34339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.07918544289961	0.650199767705845	0.20187468230745526	0.0687401070870787
8	99.10018936450956	0.6195827659860255	0.13273951713366922	0.1474883523707436
9	96.8523746941592	1.6246763815839722	0.7423799879375598	0.7805689363192702
10	60.82926642980739	26.273074684414432	5.439686481902389	7.457972403875783
11	39.16579534410876	23.473232180904997	18.845126695128407	18.51584577985783
12	31.073359652980976	15.905762843996829	27.1583998567256	25.862477646296593
13	24.99789302353756	16.640373356229144	29.86138728597728	28.500346334256015
14	26.26240811607333	16.906576663655446	30.679881798620457	26.151133421650762
15	27.324060881084883	17.47552615152848	28.958942929908794	26.241470037477843
16	32.77316291405379	18.00668174910651	25.002962935650302	24.21719240118939
17	32.395619069190474	18.69158078542815	23.970873684127355	24.94192646125402
18	31.68550215833401	19.127264012051906	23.157909984698083	26.029323844915996
19	31.860381204716465	20.029774211134843	21.605002488865946	26.504842095282743
20	30.81018512421943	21.53046819650716	21.561282727270335	26.098063952003077
21	31.807640950141035	20.747199696595388	23.804290857565757	23.640868495697816
22	30.81051433929169	19.69298719221683	24.23187539341201	25.264623075079474
23	31.626967718486874	18.81576071068316	23.731205111524897	25.826066459305064
24	32.060083067547026	19.02863117640397	22.508302804122298	26.402982951926695
25	30.019081305587964	19.45483300894675	22.18784485278819	28.338240832677098
26	27.80629511892565	19.139774184797638	23.014503899223314	30.03942679705339
27	27.793060673020957	18.42866962872441	23.991416704636137	29.786852993618496
28	30.777658675080527	20.80204692763326	20.4763873781575	27.943907019128712
29	31.459265560679604	20.299335512298157	21.36230513759873	26.879093789423504
30	31.032010239905606	18.620733701878635	23.255160117042543	27.092095941173216
31	32.32786660732016	19.968210992622947	21.60961149987753	26.09431090017936
32	35.48964816126798	19.620033132204874	20.3285698107145	24.56174889581265
33	34.646989262321206	18.9764835089586	22.104421753478487	24.27210547524171
34	29.93987215920314	21.942579623957375	25.78438783115744	22.333160385682042
35	30.40485552725769	24.14437002720633	24.04211582576358	21.408658619772396
36	30.681659560010637	25.47479397720778	20.773273530318072	23.070272932463503
37	31.223350039900865	25.205166833030017	20.087781906866372	23.483701220202743
38	32.70172324337422	24.05811567827523	20.18904846309236	23.051112615258194
39	31.895278002375616	22.699971819189816	19.914878150917456	25.489872027517112
40	29.754260701465142	21.888785881150724	21.82768356373999	26.52926985364415
41	27.68790937894235	22.62181616103621	22.118182943498795	27.572091516522647
42	26.718173462104712	20.700648685378372	23.011211748500752	29.56996610401616
43	27.125214977442187	19.13667956311843	24.273685707588537	29.464419751850844
44	27.62417334095357	19.231559346942646	23.86190349521058	29.28236381689321
45	28.392890534671615	20.280701939208463	23.79197821386338	27.53442931225655
46	28.30762383095728	22.369505729659117	22.39064133729796	26.932229102085643
47	26.99280467538077	21.437892918188737	24.208830338354083	27.360472068076408
48	29.53131625453329	20.302693506035173	24.39483685417879	25.77115338525274
49	28.390717715194725	20.117411263369426	25.481049063580645	26.0108219578552
50	26.725942937809954	20.778080070373015	23.99892280828358	28.497054183533454
51	26.046838086760022	21.214553413170183	24.45119847454904	28.287410025520753
52	27.238267433254936	21.383835803324285	24.48174963325441	26.89614713016637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	5.5
8	11.0
9	27.0
10	43.0
11	47.0
12	51.0
13	412.5
14	1289.5
15	1805.0
16	1978.5
17	2152.0
18	2209.0
19	2266.0
20	2345.0
21	2424.0
22	2451.0
23	2478.0
24	2487.5
25	2497.0
26	2807.5
27	3118.0
28	3516.0
29	3914.0
30	4962.5
31	6011.0
32	6756.0
33	7501.0
34	8264.0
35	9027.0
36	10185.0
37	11343.0
38	13779.0
39	16059.0
40	15903.0
41	18003.0
42	20103.0
43	23518.0
44	26933.0
45	31090.5
46	35248.0
47	35584.5
48	35921.0
49	42714.0
50	49507.0
51	50340.5
52	51174.0
53	53854.5
54	56535.0
55	64852.0
56	73169.0
57	84636.0
58	96103.0
59	109139.0
60	122175.0
61	140088.0
62	158001.0
63	154974.0
64	156727.0
65	161507.0
66	137836.0
67	114165.0
68	100204.5
69	86244.0
70	72824.5
71	59405.0
72	51681.5
73	43958.0
74	38441.0
75	32924.0
76	31925.0
77	30926.0
78	22847.5
79	14769.0
80	10585.0
81	6401.0
82	4707.0
83	3013.0
84	1882.5
85	752.0
86	474.0
87	196.0
88	134.5
89	57.0
90	41.0
91	34.0
92	27.0
93	18.5
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1518764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.306722333360758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.11001610584836	5.657709919120115
2	9.54697244399439	1.5860809843301589
3	4.272758391309155	1.0647785266242704
4	2.152134786661393	0.7150874438705114
5	1.1956001306935373	0.49657591537005236
6	0.7895388498301132	0.3935087998163861
7	0.5189998206734124	0.30178311809786446
8	0.37870319901424315	0.25166258567734223
9	0.31689478756816397	0.23691213082962728
>10	5.692930264047759	13.537613837772755
>50	4.456265574483768	26.366078874316575
>100	2.4242084725191697	34.792389496459954
>500	0.09506699892232039	5.299841663446295
>1k	0.04594904947912152	6.414233154460135
>5k	0.0031688999640773464	1.7666796957456912
>10k+	7.922249910193366E-4	1.1190638540622617
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17005	1.119660460743078	No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7578	0.4989583635113816	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7221	0.4754524073522943	No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6484	0.42692610570174166	No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5563	0.36628468939216363	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4793	0.3155855682647205	No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4099	0.2698905162355705	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4050	0.2666642085274605	No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3239	0.21326552380751718	No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3203	0.21089517528727308	No Hit
AGGGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2682	0.17659096475818495	No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2635	0.17349634307897738	No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2603	0.1713893666165382	No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2531	0.16664866957605	No Hit
AGGGTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2322	0.15288747955574403	No Hit
AGGGTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2141	0.14096989394007234	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAAGAACCGACTGCTC	2056	0.13537323771171822	No Hit
AGGGTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2043	0.13451727852385229	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	2033	0.13385884837934003	No Hit
AGGGTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1933	0.12727454693421755	No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1915	0.1260893726740955	No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1909	0.12569431458738817	No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1856	0.12220463482147326	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGGAAAAGCTCCGACTGCTCT	1695	0.11160390949482606	No Hit
AGGGTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1689	0.11120885140811872	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1625	0.10699489848324031	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1620	0.1066656834109842	No Hit
AGGGTCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1542	0.10152992828378866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0018436044046342948	0.0	0.0	0.0	0.0
8	0.0028970926358538917	0.0	0.0	0.0	0.0
9	0.003621365794817365	0.0	0.0	0.0	0.0
10	0.014024562078110885	0.0	0.0	0.0	0.0
11	0.023176741086831133	0.0	0.0	0.0	0.0
12	0.029431827459697493	0.0	0.0	0.0	0.0
13	0.037332989193844465	0.0	0.0	0.0	0.0
14	0.04503662188463777	0.0	0.0	0.0	0.0
15	0.054847231037870266	0.0	0.0	0.0	0.0
16	0.06676481665354196	0.0	0.0	0.0	0.0
17	0.076443739777872	0.0	0.0	0.0	0.0
18	0.08566176180104348	0.0	0.0	0.0	0.0
19	0.09876452167683722	0.0	0.0	0.0	0.0
20	0.10883850288787461	0.0	0.0	0.0	0.0
21	0.11911001314226569	0.0	0.0	0.0	0.0
22	0.12938152339665676	0.0	0.0	0.0	0.0
23	0.14149663805568213	0.0	0.0	0.0	0.0
24	0.1513072472089146	0.0	0.0	0.0	0.0
25	0.161381228419952	0.0	0.0	0.0	0.0
26	0.17086262250092837	0.0	0.0	0.0	0.0
27	0.18106828974086822	0.0	0.0	0.0	0.0
28	0.19173485808196666	0.0	0.0	0.0	0.0
29	0.2011504091484918	0.0	0.0	0.0	0.0
30	0.21056596021501695	0.0	0.0	0.0	0.0
31	0.22037656936824945	0.0	0.0	0.0	0.0
32	0.23071392263709173	0.0	0.0	0.0	0.0
33	0.2393393575302022	0.0	0.0	0.0	0.0
34	0.2513227861603251	0.0	0.0	0.0	0.0
35	0.2640304879494115	0.0	0.0	0.0	0.0
36	0.2749604283483148	0.0	0.0	0.0	0.0
37	0.2850344095593522	0.0	0.0	0.0	0.0
38	0.29379153048136514	0.0	0.0	0.0	0.0
39	0.3038655116924025	0.0	0.0	0.0	0.0
40	0.3139394929034399	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAC	35	1.0198528E-7	46.000004	16
TCGTTAA	20	6.312099E-4	46.0	45
CAACGAC	30	1.8618011E-6	46.0	37
CGCATAC	20	6.312099E-4	46.0	39
CGTTGAT	20	6.312099E-4	46.0	14
TAGCGAT	25	3.417678E-5	46.0	31
CACGCTA	20	6.312099E-4	46.0	27
TCTAGCG	25	3.417678E-5	46.0	31
CGAATCT	20	6.312099E-4	46.0	46
AACGACT	30	1.8618011E-6	46.0	38
CTCGCTA	40	5.6134013E-9	46.0	41
ACGCTAA	30	1.8618011E-6	46.0	10
ACGTAGG	20	6.312099E-4	46.0	40
ATACGGA	20	6.312099E-4	46.0	24
CGCGTAT	30	1.8618011E-6	46.0	34
TAATGCG	20	6.312099E-4	46.0	40
ATCGCGT	20	6.312099E-4	46.0	31
TATACGC	145	0.0	45.999996	19
ATACGCA	150	0.0	44.466663	20
AGGGTCG	155920	0.0	44.437855	1
>>END_MODULE