FastQCFastQC Report
Fri 17 Jun 2016
SRR1527640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531735
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC159081.0385608476662085No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG71800.4687494899574665No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT69010.4505348510022948No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT61840.40372518745083186No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC53230.34751441992250615No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT46270.30207575070100245No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC38850.25363395104244535No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA38510.2514142459367972No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT30870.20153616650399708No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC30760.20081802661687564No Hit
AGGGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG25400.16582502848077507No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG24540.16021048027237086No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG24220.15812134605529024No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG23720.15485707384110176No Hit
AGGGTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA21390.13964556532298342No Hit
AGGGTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG20770.1355978677773897No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAAGAACCGACTGCTC19710.1286776106833101No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT19390.12658847646622948No Hit
AGGGTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG19330.12619676380052686No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19040.12430348591629754No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG18990.12397705869487867No Hit
AGGGTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT18700.12208378081064936No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT17660.11529409460513732No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGGAAAAGCTCCGACTGCTCT17490.11418424205231323No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT16450.10739455584680119No Hit
AGGGTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC15830.10334685830120745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAT206.312116E-446.021
GTAATCG206.312116E-446.035
AATTCGC206.312116E-446.038
ATACGCA1050.046.020
TATACGC1050.046.019
ATCATAC405.6134013E-946.038
TTACACG206.312116E-446.041
ATCGCTA501.6370905E-1146.010
CGATAAC253.417692E-546.036
GCGTAAC206.312116E-446.09
TACGTAG405.6134013E-946.016
TACGTAC301.861812E-646.016
CCCGATA206.312116E-446.033
AGGGTCG1573350.044.397811
GGGTCGG1570850.044.1185382
GGTCGGG1529200.044.0326963
GTCGGGG947100.043.9600874
TCGGGGG401750.043.887495
TCGGGAT109950.043.615285
GTCGGGA402700.043.55554
Produced by FastQC (version 0.11.5)