##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527640_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1531735
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46451246462345	31.0	31.0	33.0	30.0	33.0
2	32.15050841039736	31.0	31.0	34.0	31.0	34.0
3	32.74822929553741	34.0	31.0	34.0	31.0	34.0
4	36.327008914727415	37.0	37.0	37.0	35.0	37.0
5	33.5231407521536	35.0	33.0	35.0	30.0	35.0
6	35.118102021563786	35.0	35.0	37.0	33.0	37.0
7	36.029079442592874	37.0	35.0	37.0	35.0	37.0
8	36.509827744355256	37.0	37.0	37.0	35.0	37.0
9	38.58931995416962	39.0	39.0	39.0	37.0	39.0
10	37.88519228195478	39.0	38.0	39.0	35.0	39.0
11	37.487981276134576	39.0	37.0	39.0	35.0	39.0
12	36.81254851524578	39.0	35.0	39.0	33.0	39.0
13	36.55229527300741	39.0	35.0	39.0	33.0	39.0
14	37.45547173629903	40.0	37.0	40.0	33.0	40.0
15	37.5040356197384	40.0	36.0	40.0	33.0	40.0
16	37.556166699853435	40.0	36.0	40.0	33.0	40.0
17	37.4489941145172	40.0	36.0	40.0	33.0	40.0
18	37.407202290213384	39.0	36.0	40.0	33.0	40.0
19	37.34459485485414	39.0	36.0	40.0	33.0	40.0
20	37.26984889683921	39.0	36.0	40.0	32.0	40.0
21	37.304923501780664	39.0	36.0	40.0	33.0	40.0
22	37.159066026434076	39.0	35.0	40.0	32.0	40.0
23	37.11819603260355	39.0	35.0	40.0	32.0	40.0
24	37.08181310735865	39.0	35.0	40.0	32.0	40.0
25	36.97144088239806	39.0	35.0	40.0	32.0	40.0
26	36.750667706881416	38.0	35.0	40.0	32.0	40.0
27	36.59418633118653	38.0	35.0	40.0	31.0	40.0
28	36.45058250937662	38.0	35.0	40.0	31.0	40.0
29	36.27996552928542	38.0	35.0	40.0	31.0	40.0
30	36.12401622996145	38.0	35.0	40.0	30.0	40.0
31	35.97246912814553	38.0	34.0	40.0	30.0	40.0
32	35.84095812918031	38.0	34.0	40.0	30.0	40.0
33	35.40952384061212	37.0	34.0	40.0	29.0	40.0
34	35.1381446529589	37.0	33.0	40.0	27.0	40.0
35	35.049957074820384	37.0	33.0	40.0	28.0	40.0
36	34.91587905218592	37.0	33.0	40.0	28.0	40.0
37	34.81969922995818	36.0	33.0	39.0	28.0	40.0
38	34.46099619059433	36.0	33.0	39.0	26.0	40.0
39	34.42869752274382	35.0	33.0	39.0	27.0	40.0
40	34.18803383091723	35.0	33.0	39.0	26.0	40.0
41	33.80777680212308	35.0	33.0	39.0	25.0	40.0
42	33.84087782808384	35.0	33.0	39.0	26.0	40.0
43	33.79274091145009	35.0	33.0	38.0	26.0	40.0
44	33.630383192915225	35.0	32.0	38.0	26.0	40.0
45	33.47395339272133	35.0	32.0	38.0	25.0	40.0
46	33.079690024710544	35.0	32.0	38.0	23.0	40.0
47	32.814414373243416	35.0	31.0	37.0	23.0	40.0
48	32.73992368131563	35.0	31.0	37.0	23.0	40.0
49	32.62630285264749	34.0	31.0	37.0	24.0	40.0
50	32.54274923534423	34.0	31.0	36.0	24.0	39.0
51	33.02472294489582	35.0	32.0	37.0	24.0	40.0
52	32.85722334476917	35.0	32.0	36.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	14.0
15	32.0
16	79.0
17	158.0
18	335.0
19	638.0
20	1160.0
21	2037.0
22	3353.0
23	4883.0
24	7296.0
25	9850.0
26	13587.0
27	18287.0
28	23407.0
29	30208.0
30	39253.0
31	50724.0
32	66762.0
33	96656.0
34	165583.0
35	177293.0
36	240753.0
37	274846.0
38	276917.0
39	27617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.05531962121385	0.6562492859404532	0.2132222610307919	0.07520883181490272
8	99.08554678191723	0.6238677055757034	0.13729528932876772	0.15329022317829127
9	96.8375077934499	1.6314832526514051	0.7584862916888365	0.772522662209847
10	60.80627523690456	26.279545743878675	5.439583217723692	7.474595801493078
11	39.04369881213134	23.587631019725997	18.82270758323078	18.545962584911884
12	31.09761153202088	15.918680450600137	27.115591143376626	25.868116874002357
13	25.18843011356403	16.663848511655083	29.872921882701643	28.274799492079243
14	26.29201526373687	16.94503292018528	30.653148227336974	26.10980358874087
15	27.299761381701142	17.47116831566818	29.031914789438122	26.197155513192556
16	32.8054787544843	17.951995612818145	25.013138695662107	24.229386937035454
17	32.34482465961801	18.617646002735462	24.03346531873986	25.004064018906664
18	31.641635139237533	19.10134585943391	23.207604448550175	26.049414552778384
19	31.796753354855767	19.966312710749577	21.74201150982383	26.49492242457083
20	30.842606586648476	21.562933536153448	21.63729365719266	25.95716622000542
21	31.810136870933942	20.71944559600714	23.772388827049067	23.69802870600985
22	30.934202064978606	19.670373791811247	24.16834504663013	25.227079096580024
23	31.56786258719687	18.88022405964478	23.750322346881152	25.801591006277196
24	31.97792046274323	18.979719076733247	22.522564281680577	26.519796178842945
25	30.026603818545638	19.431494351176934	22.253457680342876	28.28844414993455
26	27.905349162877393	19.12850460425596	23.022911926671387	29.94323430619526
27	27.80330801346186	18.450319409036158	23.98241210130995	29.763960476192032
28	30.730837906034658	20.781205626299588	20.52404626126582	27.963910206399934
29	31.343411229749275	20.272893157106157	21.432036220364488	26.95165939278008
30	31.075545051852966	18.70943733739844	23.221510248182618	26.99350736256598
31	32.37054712466582	19.96122044609544	21.647804613722347	26.02042781551639
32	35.40142387553983	19.65705556117736	20.365141489879125	24.57637907340369
33	34.47548041926312	19.12837403336739	22.150502534707375	24.245643012662114
34	30.015178865795978	21.893277884229324	25.755695338945706	22.335847911028996
35	30.317776900051246	24.20542717898331	24.0469794057066	21.42981651525884
36	30.893529233189813	25.449702461587677	20.761881134791594	22.894887170430916
37	31.1783696265999	25.21088830639765	20.057190049192585	23.55355201780987
38	32.64468070521337	23.993053628728205	20.119407077595014	23.24285858846341
39	31.885280417304564	22.746428070129625	19.909318517889844	25.45897299467597
40	29.723875213401797	21.952752923971836	21.787646035378184	26.535725827248186
41	27.878516845276764	22.553052584161097	22.13685787685207	27.43157269371007
42	26.810903974904278	20.633203524108282	23.13859773394223	29.417294767045217
43	27.14888671996135	19.15794833962794	24.239832608120857	29.453332332289854
44	27.720134357444337	19.256333504163578	23.810841953732208	29.212690184659877
45	28.3537948796626	20.23013119110029	23.835585137115757	27.580488792121354
46	28.501862267298193	22.298112924232978	22.377369453593474	26.82265535487535
47	27.102142341854176	21.46043538862793	24.25732910718891	27.180093162328994
48	29.528737020437607	20.30808201157511	24.376997326561057	25.786183641426224
49	28.438992384452927	20.05849575807826	25.456949145903174	26.045562711565644
50	26.692737320750652	20.765406548782916	24.01544653611754	28.52640959434889
51	26.054702673765373	21.21770410678087	24.464283965568455	28.2633092538853
52	27.278021328754647	21.341942307252886	24.549612041247343	26.830424322745124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.5
8	7.0
9	29.5
10	52.0
11	53.0
12	54.0
13	407.0
14	1324.0
15	1888.0
16	2043.5
17	2199.0
18	2252.0
19	2305.0
20	2338.0
21	2371.0
22	2452.5
23	2534.0
24	2518.5
25	2503.0
26	2842.0
27	3181.0
28	3581.0
29	3981.0
30	4987.0
31	5993.0
32	6711.0
33	7429.0
34	8346.0
35	9263.0
36	10390.5
37	11518.0
38	13746.0
39	16065.0
40	16156.0
41	18262.0
42	20368.0
43	23754.0
44	27140.0
45	31251.0
46	35362.0
47	35813.0
48	36264.0
49	42943.0
50	49622.0
51	50850.0
52	52078.0
53	54507.0
54	56936.0
55	65749.0
56	74562.0
57	85844.0
58	97126.0
59	110320.0
60	123514.0
61	141447.5
62	159381.0
63	156985.0
64	157807.5
65	161026.0
66	138062.5
67	115099.0
68	101018.5
69	86938.0
70	73226.5
71	59515.0
72	52098.5
73	44682.0
74	38867.5
75	33053.0
76	31856.0
77	30659.0
78	22772.5
79	14886.0
80	10711.0
81	6536.0
82	4791.0
83	3046.0
84	1900.5
85	755.0
86	498.5
87	242.0
88	170.0
89	73.0
90	48.0
91	34.5
92	21.0
93	18.0
94	15.0
95	10.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1531735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.354516217632522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.04409267734283	7.356272097945342
2	9.798625155715989	2.0292004617072643
3	3.9366792985949473	1.222872289227588
4	1.9614388362960984	0.8123900096128887
5	1.082490206768575	0.5604331200706797
6	0.6684131868687451	0.4152657110107116
7	0.4625116161028911	0.3352358820846568
8	0.33261608717837243	0.27552629351471436
9	0.29733933044679284	0.27709244273261807
>10	4.95451530428036	14.63028061482011
>50	3.559485159256122	26.107575563641593
>100	1.7868460514625706	31.981853618475263
>500	0.08007550067992728	5.56629152399664
>1k	0.03164273817190675	5.655023205807483
>5k	0.002583080667094428	1.7109768456487002
>10k+	6.45770166773607E-4	1.0637103197037487
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	15908	1.0385608476662085	No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7180	0.4687494899574665	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6901	0.4505348510022948	No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6184	0.40372518745083186	No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5323	0.34751441992250615	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4627	0.30207575070100245	No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3885	0.25363395104244535	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3851	0.2514142459367972	No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3087	0.20153616650399708	No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3076	0.20081802661687564	No Hit
AGGGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2540	0.16582502848077507	No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2454	0.16021048027237086	No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2422	0.15812134605529024	No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2372	0.15485707384110176	No Hit
AGGGTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2139	0.13964556532298342	No Hit
AGGGTCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2077	0.1355978677773897	No Hit
AGGGTCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAAGAACCGACTGCTC	1971	0.1286776106833101	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	1939	0.12658847646622948	No Hit
AGGGTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1933	0.12619676380052686	No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1904	0.12430348591629754	No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1899	0.12397705869487867	No Hit
AGGGTCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1870	0.12208378081064936	No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1766	0.11529409460513732	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGCGGGGAAAAGCTCCGACTGCTCT	1749	0.11418424205231323	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1645	0.10739455584680119	No Hit
AGGGTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1583	0.10334685830120745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.002023848772796861	0.0	0.0	0.0	0.0
8	0.003590699435607334	0.0	0.0	0.0	0.0
9	0.004308839322728801	0.0	0.0	0.0	0.0
10	0.014558654075280645	0.0	0.0	0.0	0.0
11	0.024220899829278563	0.0	0.0	0.0	0.0
12	0.03159815503334454	0.0	0.0	0.0	0.0
13	0.03864898301599167	0.0	0.0	0.0	0.0
14	0.046548521774327806	0.0	0.0	0.0	0.0
15	0.05575376941833934	0.0	0.0	0.0	0.0
16	0.06633001139231003	0.0	0.0	0.0	0.0
17	0.0769715388105645	0.0	0.0	0.0	0.0
18	0.08728663900740011	0.0	0.0	0.0	0.0
19	0.1008660114184242	0.0	0.0	0.0	0.0
20	0.11150753883667867	0.0	0.0	0.0	0.0
21	0.12110449914639282	0.0	0.0	0.0	0.0
22	0.13030974679040436	0.0	0.0	0.0	0.0
23	0.14055956154295618	0.0	0.0	0.0	0.0
24	0.15120108896121065	0.0	0.0	0.0	0.0
25	0.16288718348800543	0.0	0.0	0.0	0.0
26	0.17392042357196252	0.0	0.0	0.0	0.0
27	0.18266867310598767	0.0	0.0	0.0	0.0
28	0.19370191318994473	0.0	0.0	0.0	0.0
29	0.20362530072107773	0.0	0.0	0.0	0.0
30	0.21224297936653533	0.0	0.0	0.0	0.0
31	0.22268865045193847	0.0	0.0	0.0	0.0
32	0.23372189053589557	0.0	0.0	0.0	0.0
33	0.24168671473851547	0.0	0.0	0.0	0.0
34	0.25271995482247256	0.0	0.0	0.0	0.0
35	0.26407962212784847	0.0	0.0	0.0	0.0
36	0.27478643499038674	0.0	0.0	0.0	0.0
37	0.2851015351872223	0.0	0.0	0.0	0.0
38	0.2935233574998286	0.0	0.0	0.0	0.0
39	0.3025980342552726	0.0	0.0	0.0	0.0
40	0.31323956167352707	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAT	20	6.312116E-4	46.0	21
GTAATCG	20	6.312116E-4	46.0	35
AATTCGC	20	6.312116E-4	46.0	38
ATACGCA	105	0.0	46.0	20
TATACGC	105	0.0	46.0	19
ATCATAC	40	5.6134013E-9	46.0	38
TTACACG	20	6.312116E-4	46.0	41
ATCGCTA	50	1.6370905E-11	46.0	10
CGATAAC	25	3.417692E-5	46.0	36
GCGTAAC	20	6.312116E-4	46.0	9
TACGTAG	40	5.6134013E-9	46.0	16
TACGTAC	30	1.861812E-6	46.0	16
CCCGATA	20	6.312116E-4	46.0	33
AGGGTCG	157335	0.0	44.39781	1
GGGTCGG	157085	0.0	44.118538	2
GGTCGGG	152920	0.0	44.032696	3
GTCGGGG	94710	0.0	43.960087	4
TCGGGGG	40175	0.0	43.88749	5
TCGGGAT	10995	0.0	43.61528	5
GTCGGGA	40270	0.0	43.5555	4
>>END_MODULE