FastQCFastQC Report
Fri 17 Jun 2016
SRR1527639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948932
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC108300.5556889619545474No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG73520.377232248226208No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT63620.3264351963023851No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC59960.30765568013660816No Hit
GGGTTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC42420.21765767097056232No Hit
GGGTTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT40930.21001245810525973No Hit
GGGTTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC36250.18599930628672526No Hit
GGGTTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA32770.16814337288319964No Hit
GGGTTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC31770.16301235753735893No Hit
GGGTTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG31540.16183222400781558No Hit
GGGTTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA29350.15059530040042443No Hit
GGGTTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA27140.1392557564861165No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT25380.13022516947743687No Hit
GGGTTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG24600.12622297750768113No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC23840.1223234058448422No Hit
GGGTTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG23630.12124589262221565No Hit
GGGTTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC23320.11965527786500503No Hit
GGGTTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT23250.1192961067907962No Hit
GGGTTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC22460.11524260466758203No Hit
GGGTTTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG21710.11139434315820151No Hit
GGGTTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT20040.10282554753064754No Hit
GGGTTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG19940.1023124459960635No Hit
GGGTTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG19520.1001574195508104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA207.8578317E-444.035
GTTACGC254.4439847E-544.031
CGTAATT207.8578317E-444.040
TAATGCG207.8578317E-444.021
GTTTGGG1980550.042.1860583
GGTTTGG2014450.042.1827282
TTGGGAT210150.042.1784445
TTTGGGA704300.042.0102234
GGGTTTG2032150.042.0058561
TTTGGGG1037300.041.9915164
TTGGGGG348900.041.5345425
TTGGGGC229400.041.4777685
TTGGGGT184300.041.32615
TTGGGAC132250.041.2884675
TTGGGAG245650.041.2595185
TGGGATC59300.040.9207466
TGGGGGG151400.040.8177036
TGGGATT73900.040.8146176
TTTGGGC157600.040.747464
TGGGACT53500.040.422436
Produced by FastQC (version 0.11.5)