FastQCFastQC Report
Fri 17 Jun 2016
SRR1527638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928204
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC164660.5623242096520598No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT103170.352332009655065No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG96730.330339006435344No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA88810.30329171055022125No Hit
GGGTTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT66470.2269992118035492No Hit
GGGTTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT61800.2110508694066397No Hit
GGGTTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC55520.18960427620479994No Hit
GGGTTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC49110.16771372486343164No Hit
GGGTTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA48140.16440111413002645No Hit
GGGTTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC47840.1633765953464991No Hit
GGGTTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA47560.16242037781520688No Hit
GGGTTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA39880.136192696956906No Hit
GGGTTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC39600.1352364794256138No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT37620.1284746554543331No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT36960.12622071413057287No Hit
GGGTTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC36280.12389847155457748No Hit
GGGTTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT36060.12314715777999073No Hit
GGGTTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT33940.11590722504306394No Hit
GGGTTTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG33580.11467780250283108No Hit
GGGTTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG33380.1139947899804795No Hit
GGGTTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG32380.11057972736872158No Hit
GGGTTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG30970.1057644890861429No Hit
GGGTTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG29920.1021786733437971No Hit
GGGTTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT29450.10057359391627085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC206.313062E-446.033
TAACGAA206.313062E-446.041
AATTCGG206.313062E-446.027
GTTTGGG2982650.044.0020143
TTGGGAT325400.043.9996955
GGTTTGG3037400.043.9888082
TTTGGGG1566550.043.8696484
GGGTTTG3062900.043.803551
TTTGGGA1060450.043.763874
GTAACGA900.043.44444722
TTGGGGC350650.043.271355
TTGGGGG520750.043.173315
TTGGGAC201250.043.062865
TGGGATC93250.042.867566
TTGGGGT279950.042.7219165
TTGGGAG368050.042.612965
TTTGGGC233950.042.5394334
TTGGGGA450450.042.4206925
TGGGGGG220300.042.272816
TGGGATT115600.042.2595186
Produced by FastQC (version 0.11.5)