##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2928204
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.664742620391202	31.0	31.0	33.0	30.0	33.0
2	32.46081352255512	33.0	31.0	34.0	31.0	34.0
3	32.998494981907	34.0	31.0	34.0	31.0	34.0
4	33.230182391663966	35.0	33.0	35.0	30.0	35.0
5	34.88220287930759	35.0	35.0	35.0	33.0	37.0
6	35.847335431547805	37.0	35.0	37.0	35.0	37.0
7	35.88929186627708	35.0	35.0	37.0	35.0	37.0
8	36.452923361896914	37.0	37.0	37.0	35.0	37.0
9	38.67431128432309	39.0	39.0	39.0	38.0	39.0
10	38.05134785691161	39.0	38.0	39.0	37.0	39.0
11	37.76523425280479	39.0	38.0	39.0	35.0	39.0
12	37.40160555753629	39.0	37.0	39.0	35.0	39.0
13	37.28089948651119	39.0	37.0	39.0	34.0	39.0
14	38.08829200424561	40.0	38.0	40.0	34.0	40.0
15	38.138771410735046	40.0	38.0	40.0	34.0	40.0
16	38.16307846038049	40.0	38.0	40.0	34.0	40.0
17	38.11385784596975	40.0	38.0	40.0	34.0	40.0
18	38.06856387055	40.0	38.0	40.0	34.0	40.0
19	38.07975024964108	40.0	38.0	40.0	34.0	40.0
20	38.03841467329462	40.0	38.0	40.0	34.0	40.0
21	38.009632867108984	40.0	38.0	40.0	34.0	40.0
22	38.01108256118768	40.0	38.0	40.0	34.0	40.0
23	37.94361560874857	40.0	38.0	40.0	34.0	40.0
24	37.90736710966859	40.0	37.0	40.0	34.0	40.0
25	37.852910179755234	40.0	37.0	40.0	34.0	40.0
26	37.73076636737058	40.0	37.0	40.0	34.0	40.0
27	37.626636327250424	40.0	37.0	40.0	33.0	40.0
28	37.49832559480146	40.0	36.0	40.0	33.0	40.0
29	37.43932868065203	40.0	36.0	40.0	33.0	40.0
30	37.333687133819915	40.0	36.0	40.0	33.0	40.0
31	37.22157096978216	39.0	36.0	40.0	33.0	40.0
32	37.10995818597338	39.0	36.0	40.0	32.0	40.0
33	36.969370986447665	39.0	35.0	40.0	32.0	40.0
34	36.845498469368934	39.0	35.0	40.0	31.0	40.0
35	36.714133987932534	39.0	35.0	40.0	31.0	40.0
36	36.70390382637275	39.0	35.0	40.0	31.0	40.0
37	36.59878717466406	39.0	35.0	40.0	31.0	40.0
38	36.51453553099442	39.0	35.0	40.0	31.0	40.0
39	36.39146828567955	38.0	35.0	40.0	31.0	40.0
40	36.14197986205879	38.0	35.0	40.0	30.0	40.0
41	36.008958733749424	38.0	35.0	40.0	30.0	40.0
42	35.89992295618748	38.0	35.0	40.0	30.0	40.0
43	35.796046996725636	38.0	35.0	40.0	30.0	40.0
44	35.64519992459542	38.0	34.0	40.0	29.0	40.0
45	35.5455538616845	38.0	34.0	40.0	29.0	40.0
46	35.41216254058802	37.0	34.0	40.0	29.0	40.0
47	35.19646103891669	37.0	34.0	40.0	28.0	40.0
48	35.01791166189241	37.0	34.0	40.0	28.0	40.0
49	35.329089776531966	37.0	34.0	40.0	29.0	40.0
50	35.41024054335012	37.0	34.0	40.0	29.0	40.0
51	35.400323884538096	37.0	34.0	40.0	30.0	40.0
52	34.92786704751445	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	6.0
15	13.0
16	54.0
17	128.0
18	299.0
19	588.0
20	1131.0
21	1965.0
22	3265.0
23	5230.0
24	7732.0
25	11260.0
26	15616.0
27	21236.0
28	28589.0
29	37766.0
30	48693.0
31	62515.0
32	82059.0
33	113834.0
34	203841.0
35	214015.0
36	319552.0
37	504638.0
38	1035655.0
39	208521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.62133785760828	0.15285820250228468	0.19909815026548697	0.026705789623946964
8	99.4867502400789	0.28911920071142583	0.10976011234189967	0.11437044686777287
9	98.03565598571684	0.9345660343336735	0.458984415020265	0.5707935649292194
10	51.9090541505988	35.2444706721253	5.017205085438036	7.829270091837865
11	32.5581482710904	23.756780606815646	23.80479638713696	19.88027473495699
12	29.354785390635353	17.522413055920968	27.37001930193388	25.7527822515098
13	24.923775802505563	18.477367014046838	29.424725872924157	27.174131310523446
14	26.033466247570182	19.30640761367719	29.754211113706557	24.90591502504607
15	26.57075804827806	19.71481495141732	28.19557653770024	25.51885046260438
16	31.25878524856875	20.293941269119227	25.679495007861476	22.76777847445055
17	31.71903323675536	20.458103328866432	22.96513494278404	24.857728491594163
18	28.968166152358236	20.57086869630668	25.57151072807769	24.889454423257394
19	29.07242801389521	21.29100294924807	24.72706819606831	24.909500840788414
20	28.99466703822548	21.99187624905915	24.848507822542416	24.16494889017295
21	30.135707758066037	19.950932380394264	26.08216504041385	23.83119482112585
22	29.959661280429916	19.7190496290559	25.331261073340517	24.990028017173664
23	29.41338786505312	21.33714044513292	25.354722553483295	23.894749136330663
24	29.031925371319755	21.31149332491862	24.396114478362847	25.260466825398776
25	27.838156084753656	21.382492476617067	24.3245347660204	26.454816672608878
26	27.32996061749796	22.108364034746213	23.689913680877424	26.871761666878403
27	26.769992801048016	20.12322228915745	24.839492057247377	28.26729285254716
28	28.283480249326892	22.595932523826892	22.836523684825238	26.28406354202098
29	29.82350273409913	22.056181878038554	23.08701852739768	25.033296860464638
30	29.020006802804723	21.14077434495684	23.731645745993106	26.10757310624533
31	29.985001045009156	21.808384934929396	23.45379625190048	24.752817768160963
32	31.345254633898456	21.90540686372944	23.369785711651236	23.379552790720865
33	30.8765714410608	21.546415481981445	23.716243813614078	23.860769263343673
34	28.54316161032496	22.834884454771593	25.712928470830583	22.909025464072858
35	27.936100080458875	24.651458709843986	25.288231284432367	22.124209925264772
36	28.404475917661475	24.82887121252481	22.784512281248166	23.98214058856555
37	29.096845711569273	25.716138629685638	21.968961178934254	23.21805447981083
38	29.56088441925494	24.752817768160963	22.28615219431433	23.400145618269764
39	28.557675626424935	23.790521425419815	22.117653005050194	25.534149943105056
40	27.56659030586667	23.786628254042412	23.013355626862065	25.63342581322886
41	25.68140744292406	23.646678988212567	23.801210571394616	26.870702997468754
42	25.733111490866072	22.80732489949471	23.79198990234287	27.66757370729635
43	25.551259406789967	22.320097916675206	25.40010873559356	26.728533940941272
44	26.42035869085624	21.687457567847048	25.03220404042888	26.859979700867836
45	26.720850050064822	22.157062827589883	24.27166959679039	26.850417525554914
46	26.437468154541143	23.368419686606533	23.531625528822445	26.662486630029875
47	25.77446789909446	23.154363562101548	25.216310065828747	25.854858472975245
48	27.667061447904583	22.355102308445723	24.52841400394235	25.449422239707342
49	26.99419166150992	22.809715443322936	24.755993776389897	25.440099118777244
50	25.471654297309886	23.4544109631706	25.158254001428865	25.91568073809065
51	25.16972861180437	24.130729962803137	24.4766416547481	26.222899770644393
52	25.841232373154327	24.008743926311148	24.642613697679533	25.50741000285499
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	22.5
6	45.0
7	27.5
8	10.0
9	35.5
10	61.0
11	852.0
12	1643.0
13	3611.0
14	6906.0
15	8233.0
16	7341.0
17	6449.0
18	5895.5
19	5342.0
20	6178.0
21	7014.0
22	6675.5
23	6337.0
24	6994.0
25	7651.0
26	10159.5
27	12668.0
28	13647.5
29	14627.0
30	19242.5
31	23858.0
32	24363.5
33	24869.0
34	29448.0
35	34027.0
36	39586.0
37	45145.0
38	50239.5
39	62659.0
40	69984.0
41	71210.0
42	72436.0
43	78082.0
44	83728.0
45	94210.5
46	104693.0
47	109852.5
48	115012.0
49	125937.0
50	136862.0
51	141839.5
52	146817.0
53	157776.5
54	168736.0
55	183181.5
56	197627.0
57	210324.5
58	223022.0
59	243250.5
60	263479.0
61	257072.5
62	250666.0
63	244517.0
64	208279.0
65	178190.0
66	158639.5
67	139089.0
68	117340.5
69	95592.0
70	80454.5
71	65317.0
72	55451.0
73	45585.0
74	43297.5
75	41010.0
76	30568.0
77	20126.0
78	14039.0
79	7952.0
80	5705.0
81	3458.0
82	2242.5
83	1027.0
84	695.0
85	363.0
86	250.0
87	137.0
88	77.5
89	16.0
90	14.0
91	8.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2928204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.885546558880353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.93627671728017	5.35714673091092
2	9.59145752998917	1.512677698405063
3	4.279205497142899	1.0123162254821125
4	2.163361810295554	0.6823716111515711
5	1.1638140120223222	0.45886547888396806
6	0.7428011846054755	0.3514435995118774
7	0.4888634431701434	0.2698468809436908
8	0.3957850177690593	0.2496784947940164
9	0.2797563460920745	0.19854285230261692
>10	5.450124060397152	12.34553413192357
>50	4.599881899493438	26.14689618572533
>100	2.769360784714273	38.180646064264714
>500	0.0999410000420242	5.2644372519359335
>1k	0.03634218183346335	5.795834681985925
>5k	0.0021632251091347225	1.2600219108908797
>10k+	8.652900436538892E-4	0.9137402008878354
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16466	0.5623242096520598	No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	10317	0.352332009655065	No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9673	0.330339006435344	No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8881	0.30329171055022125	No Hit
GGGTTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6647	0.2269992118035492	No Hit
GGGTTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6180	0.2110508694066397	No Hit
GGGTTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5552	0.18960427620479994	No Hit
GGGTTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4911	0.16771372486343164	No Hit
GGGTTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	4814	0.16440111413002645	No Hit
GGGTTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	4784	0.1633765953464991	No Hit
GGGTTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	4756	0.16242037781520688	No Hit
GGGTTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	3988	0.136192696956906	No Hit
GGGTTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3960	0.1352364794256138	No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3762	0.1284746554543331	No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3696	0.12622071413057287	No Hit
GGGTTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3628	0.12389847155457748	No Hit
GGGTTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	3606	0.12314715777999073	No Hit
GGGTTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	3394	0.11590722504306394	No Hit
GGGTTTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3358	0.11467780250283108	No Hit
GGGTTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	3338	0.1139947899804795	No Hit
GGGTTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3238	0.11057972736872158	No Hit
GGGTTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3097	0.1057644890861429	No Hit
GGGTTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2992	0.1021786733437971	No Hit
GGGTTTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	2945	0.10057359391627085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0014001756708207488	0.0	0.0	0.0	0.0
8	0.003859020751286454	0.0	0.0	0.0	0.0
9	0.007239932736926799	0.0	0.0	0.0	0.0
10	0.047298617172847245	0.0	0.0	0.0	0.0
11	0.07017953667162534	0.0	0.0	0.0	0.0
12	0.08465940214547893	0.0	0.0	0.0	0.0
13	0.09490458998075271	0.0	0.0	0.0	0.0
14	0.10818918354049104	0.0	0.0	0.0	0.0
15	0.1222250908748161	0.0	0.0	0.0	0.0
16	0.13892474704631236	0.0	0.0	0.0	0.0
17	0.1531655581373429	0.0	0.0	0.0	0.0
18	0.16576713917472963	0.0	0.0	0.0	0.0
19	0.18041775777917113	0.0	0.0	0.0	0.0
20	0.1939414057217325	0.0	0.0	0.0	0.0
21	0.20531356421888639	0.0	0.0	0.0	0.0
22	0.21620761395039417	0.0	0.0	0.0	0.0
23	0.23089238318095323	0.0	0.0	0.0	0.0
24	0.24571375491598263	0.0	0.0	0.0	0.0
25	0.25838363720560453	0.0	0.0	0.0	0.0
26	0.2737172683323976	0.0	0.0	0.0	0.0
27	0.28577243935190305	0.0	0.0	0.0	0.0
28	0.30561395312621664	0.0	0.0	0.0	0.0
29	0.31739591913678145	0.0	0.0	0.0	0.0
30	0.33419802718663044	0.0	0.0	0.0	0.0
31	0.34666300571954684	0.0	0.0	0.0	0.0
32	0.36329436063880793	0.0	0.0	0.0	0.0
33	0.37497387477102007	0.0	0.0	0.0	0.0
34	0.388531673339699	0.0	0.0	0.0	0.0
35	0.4098075134109509	0.0	0.0	0.0	0.0
36	0.4242532282586869	0.0	0.0	0.0	0.0
37	0.43996251627277333	0.0	0.0	0.0	0.0
38	0.45283730231910074	0.0	0.0	0.0	0.0
39	0.4655413352348402	0.0	0.0	0.0	0.0
40	0.4787917781684609	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	20	6.313062E-4	46.0	33
TAACGAA	20	6.313062E-4	46.0	41
AATTCGG	20	6.313062E-4	46.0	27
GTTTGGG	298265	0.0	44.002014	3
TTGGGAT	32540	0.0	43.999695	5
GGTTTGG	303740	0.0	43.988808	2
TTTGGGG	156655	0.0	43.869648	4
GGGTTTG	306290	0.0	43.80355	1
TTTGGGA	106045	0.0	43.76387	4
GTAACGA	90	0.0	43.444447	22
TTGGGGC	35065	0.0	43.27135	5
TTGGGGG	52075	0.0	43.17331	5
TTGGGAC	20125	0.0	43.06286	5
TGGGATC	9325	0.0	42.86756	6
TTGGGGT	27995	0.0	42.721916	5
TTGGGAG	36805	0.0	42.61296	5
TTTGGGC	23395	0.0	42.539433	4
TTGGGGA	45045	0.0	42.420692	5
TGGGGGG	22030	0.0	42.27281	6
TGGGATT	11560	0.0	42.259518	6
>>END_MODULE