FastQCFastQC Report
Fri 17 Jun 2016
SRR1527637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949277
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC155050.5257220667980661No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT99570.3376081663404285No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG93440.3168234112970738No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA84380.2861040180356067No Hit
GGGTTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT62360.21144165163190842No Hit
GGGTTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT58240.19747212621940902No Hit
GGGTTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC52460.1778741027038152No Hit
GGGTTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC46240.15678418812475056No Hit
GGGTTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC46150.15647902858904064No Hit
GGGTTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA45410.15396993907320336No Hit
GGGTTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA44680.15149475617244498No Hit
GGGTTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC38430.1303031217481437No Hit
GGGTTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA37590.1274549660815176No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT35490.12033457691495238No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT34830.11809674031974615No Hit
GGGTTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT33960.11514686480788341No Hit
GGGTTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC33160.11243433560157287No Hit
GGGTTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT32870.11145104376428527No Hit
GGGTTTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG32360.1097218063952623No Hit
GGGTTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG31770.10772131610560826No Hit
GGGTTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG29800.10104171293506849No Hit
GGGTTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG29660.10056702032396414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATA405.6170393E-946.013
TTGGGAT326700.044.1202935
GTTTGGG3000600.043.97183
GGTTTGG3061650.043.947642
TTTGGGA1071300.043.896014
TTTGGGG1563500.043.775764
GGGTTTG3089400.043.7375221
TTGGGGC356650.043.2140775
TTGGGGG528100.043.1516765
TTGGGAC198300.043.1351475
TTGGGGT275400.042.909955
TGGGATC99700.042.8856546
TTGGGAG372700.042.6367035
TGGGATT110050.042.593376
TTGGGGA436050.042.255025
TTTGGGC238650.042.1546174
TGGGGGG229400.042.0998276
TGGGACT75950.041.8512156
TGGGGCT194300.041.513646
TGGGATG89600.041.4051366
Produced by FastQC (version 0.11.5)