##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2949277
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.643123382442543	31.0	31.0	33.0	30.0	33.0
2	32.431872964119684	33.0	31.0	34.0	31.0	34.0
3	32.98548389995243	34.0	31.0	34.0	31.0	34.0
4	33.18745000893439	35.0	33.0	35.0	30.0	35.0
5	34.87750048571226	35.0	35.0	35.0	33.0	37.0
6	35.79157230738245	37.0	35.0	37.0	35.0	37.0
7	35.82600617032581	35.0	35.0	37.0	35.0	37.0
8	36.41154187958608	37.0	35.0	37.0	35.0	37.0
9	38.625834399413826	39.0	39.0	39.0	38.0	39.0
10	37.96098467522718	39.0	38.0	39.0	35.0	39.0
11	37.73225641402961	39.0	38.0	39.0	35.0	39.0
12	37.352796973631165	39.0	37.0	39.0	35.0	39.0
13	37.079882289795094	39.0	37.0	39.0	34.0	39.0
14	38.02799364047527	40.0	38.0	40.0	34.0	40.0
15	38.09578313600248	40.0	38.0	40.0	34.0	40.0
16	38.14683802165751	40.0	38.0	40.0	34.0	40.0
17	38.136640946238686	40.0	38.0	40.0	34.0	40.0
18	38.100613811452774	40.0	38.0	40.0	34.0	40.0
19	38.063055792996046	40.0	38.0	40.0	34.0	40.0
20	38.03619666786131	40.0	38.0	40.0	34.0	40.0
21	38.06588190936287	40.0	38.0	40.0	34.0	40.0
22	37.95802225426774	40.0	38.0	40.0	34.0	40.0
23	37.91666805118678	40.0	38.0	40.0	34.0	40.0
24	37.93069080998495	40.0	37.0	40.0	34.0	40.0
25	37.847001824514955	40.0	37.0	40.0	34.0	40.0
26	37.673539311499056	40.0	37.0	40.0	34.0	40.0
27	37.572718669694304	40.0	37.0	40.0	33.0	40.0
28	37.464452813350526	40.0	36.0	40.0	33.0	40.0
29	37.40551599595426	40.0	36.0	40.0	33.0	40.0
30	37.293946618103355	40.0	36.0	40.0	33.0	40.0
31	37.19947261650906	39.0	36.0	40.0	32.0	40.0
32	37.094832733581825	39.0	36.0	40.0	32.0	40.0
33	36.80029987010376	39.0	35.0	40.0	31.0	40.0
34	36.76746334779676	39.0	35.0	40.0	31.0	40.0
35	36.703175049342605	39.0	35.0	40.0	31.0	40.0
36	36.554140218094126	39.0	35.0	40.0	31.0	40.0
37	36.527162419806615	39.0	35.0	40.0	31.0	40.0
38	36.16483734827214	38.0	35.0	40.0	30.0	40.0
39	36.220798521129076	38.0	35.0	40.0	30.0	40.0
40	36.047211570835834	38.0	35.0	40.0	30.0	40.0
41	35.72596639786632	38.0	35.0	40.0	29.0	40.0
42	35.75296793078439	38.0	35.0	40.0	29.0	40.0
43	35.76259469693759	38.0	34.0	40.0	30.0	40.0
44	35.61748218292144	38.0	34.0	40.0	29.0	40.0
45	35.5130572679338	38.0	34.0	40.0	29.0	40.0
46	35.2037655330442	37.0	34.0	40.0	28.0	40.0
47	34.90649911825847	37.0	34.0	40.0	27.0	40.0
48	34.893572899391955	37.0	33.0	40.0	27.0	40.0
49	34.810691908559285	36.0	33.0	40.0	27.0	40.0
50	34.66352906152932	36.0	33.0	40.0	27.0	40.0
51	34.99496656299154	36.0	34.0	40.0	28.0	40.0
52	34.73171255192374	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	7.0
15	15.0
16	52.0
17	128.0
18	311.0
19	641.0
20	1186.0
21	1985.0
22	3436.0
23	5399.0
24	8183.0
25	11668.0
26	16279.0
27	22333.0
28	30011.0
29	39757.0
30	51256.0
31	65066.0
32	87266.0
33	121278.0
34	210026.0
35	223137.0
36	338773.0
37	536864.0
38	999062.0
39	175155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.6112945647357	0.15763185350172262	0.20303281109234567	0.02804077067023545
8	99.48970544306283	0.2922072087498055	0.10843335502226478	0.10965399316510453
9	97.99611904883807	0.9396540236810582	0.4789648446042878	0.585262082876583
10	51.85026703154705	35.231312623398885	5.069344113828576	7.849076231225484
11	32.629759768241506	23.799731256168887	23.73778387041977	19.832725105169843
12	29.366824479355447	17.574069848305196	27.313880656174376	25.74522501616498
13	25.195395346045828	18.446995653511014	29.324814183272714	27.032794817170448
14	26.023293166426892	19.294728843713223	29.80689843646426	24.87507955339563
15	26.565154782002505	19.733751695754588	28.21396566005838	25.48712786218453
16	31.276682386903637	20.288565638290336	25.69351742816968	22.741234546636345
17	31.73425215739315	20.479019095188413	22.925076213594043	24.86165253382439
18	29.002972592943966	20.559920278766626	25.572097839572205	24.865009288717204
19	29.043185838427522	21.28318228501426	24.736231964647608	24.937399911910614
20	29.014670375146178	21.970571092508433	24.85070069715391	24.16405783519147
21	30.108769030511546	19.893553572621357	26.1497987472862	23.8478786495809
22	30.13175771553503	19.71913794465559	25.232692622632598	24.916411717176786
23	29.347870681526356	21.31227416075194	25.497774539319295	23.84208061840241
24	29.040235962915656	21.291523312323665	24.409948607743527	25.258292117017156
25	27.897786474447805	21.35835325064414	24.356579595609364	26.38728067929869
26	27.39105211209391	22.088159233602	23.6961465471029	26.824642107201193
27	26.783547289725583	20.157550477625534	24.84944615239599	28.209456080252888
28	28.291069302747758	22.605099487094634	22.898052641376175	26.205778568781433
29	29.867930343606247	22.028076711682218	23.096135086667	25.00785785804453
30	29.03562466326493	21.08913472691782	23.76409540372098	26.111145206096275
31	29.927877239065708	21.79656234392361	23.54631321506932	24.729247201941355
32	31.264679445165715	21.92072836834248	23.488909315740774	23.325682870751034
33	30.864072788008723	21.527072567276658	23.78406640000244	23.824788244712177
34	28.546521740752056	22.81172639938534	25.744784230168953	22.896967629693652
35	27.9671594088992	24.654720461997975	25.280297510203347	22.097822618899478
36	28.561406744771684	24.797026525484043	22.768597185005003	23.87296954473927
37	29.058748974748728	25.708911031415493	22.055134190515165	23.17720580332061
38	29.53913789718633	24.772918922162958	22.230770456623773	23.45717272402694
39	28.504138471903453	23.800104228934753	22.186759670251387	25.508997628910407
40	27.534544907107744	23.8247543380971	23.02015036227523	25.62055039251993
41	25.978027835296587	23.54872058473992	23.793763691915	26.67948788804849
42	25.717998004256636	22.776327893242986	23.83024720973988	27.675426892760495
43	25.52629000260064	22.31930062859474	25.373574608285352	26.780834760519273
44	26.400233006258823	21.71142283346054	25.05129223196058	26.83705192832006
45	26.714479514809902	22.11419951398258	24.332539805518437	26.838781165689085
46	26.578242735422954	23.354164427417295	23.505387930669112	26.562204906490646
47	25.978638154368	23.183376807264967	25.127819462193617	25.710165576173416
48	27.631619546078586	22.346731080193553	24.548219784035204	25.47342958969266
49	26.999464614547904	22.876081154805057	24.681676220985686	25.44277800966135
50	25.540361247858375	23.3976666145635	25.134499065364157	25.92747307221397
51	25.209093618537697	24.099567453311437	24.4713195810363	26.220019347114565
52	25.87942739864719	23.948072697138993	24.633427107728437	25.539072796485375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	16.0
6	32.0
7	21.0
8	10.0
9	41.0
10	72.0
11	853.0
12	1634.0
13	3518.0
14	6786.5
15	8171.0
16	7433.0
17	6695.0
18	6077.0
19	5459.0
20	6213.0
21	6967.0
22	6691.5
23	6416.0
24	7054.0
25	7692.0
26	10112.5
27	12533.0
28	13619.0
29	14705.0
30	19144.0
31	23583.0
32	24365.5
33	25148.0
34	29513.5
35	33879.0
36	39870.5
37	45862.0
38	50970.5
39	63352.5
40	70626.0
41	71975.5
42	73325.0
43	78850.5
44	84376.0
45	94620.5
46	104865.0
47	110700.0
48	116535.0
49	126977.0
50	137419.0
51	142505.0
52	147591.0
53	158967.5
54	170344.0
55	184967.0
56	199590.0
57	212403.5
58	225217.0
59	245365.5
60	265514.0
61	259566.0
62	253618.0
63	246418.0
64	209451.5
65	179685.0
66	159589.5
67	139494.0
68	117405.5
69	95317.0
70	80648.5
71	65980.0
72	55971.0
73	45962.0
74	43330.5
75	40699.0
76	30561.5
77	20424.0
78	14218.0
79	8012.0
80	5767.0
81	3522.0
82	2292.0
83	1062.0
84	703.0
85	344.0
86	239.5
87	135.0
88	84.5
89	26.5
90	19.0
91	14.0
92	9.0
93	5.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2949277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.168942911769046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.2889659207305	5.496797211984203
2	9.960213336480503	1.6272882826949984
3	4.344187620241372	1.0646226200329678
4	2.232014854865105	0.7293280771045405
5	1.2352639681223927	0.5045400418278562
6	0.7644473917649681	0.3746836261427263
7	0.4948834362684033	0.2829872177199677
8	0.4007697359916723	0.26190920752645785
9	0.30705043043349844	0.22574496935208252
>10	5.710318489211021	13.448313202274514
>50	4.535033370448127	26.416403694900346
>100	2.596296795963893	36.824811118493436
>500	0.09182343679992981	4.923573792642198
>1k	0.03568493752414334	5.666457599952369
>5k	0.002611092989571464	1.601339957902409
>10k+	4.3518216492857735E-4	0.5511993794489255
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	15505	0.5257220667980661	No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9957	0.3376081663404285	No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9344	0.3168234112970738	No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8438	0.2861040180356067	No Hit
GGGTTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6236	0.21144165163190842	No Hit
GGGTTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5824	0.19747212621940902	No Hit
GGGTTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5246	0.1778741027038152	No Hit
GGGTTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4624	0.15678418812475056	No Hit
GGGTTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	4615	0.15647902858904064	No Hit
GGGTTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	4541	0.15396993907320336	No Hit
GGGTTTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	4468	0.15149475617244498	No Hit
GGGTTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3843	0.1303031217481437	No Hit
GGGTTTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	3759	0.1274549660815176	No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3549	0.12033457691495238	No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3483	0.11809674031974615	No Hit
GGGTTTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	3396	0.11514686480788341	No Hit
GGGTTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3316	0.11243433560157287	No Hit
GGGTTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	3287	0.11145104376428527	No Hit
GGGTTTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3236	0.1097218063952623	No Hit
GGGTTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	3177	0.10772131610560826	No Hit
GGGTTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2980	0.10104171293506849	No Hit
GGGTTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2966	0.10056702032396414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0018987704444173945	0.0	0.0	0.0	0.0
8	0.004814739341201251	0.0	0.0	0.0	0.0
9	0.008578373614957157	0.0	0.0	0.0	0.0
10	0.045977370046964054	0.0	0.0	0.0	0.0
11	0.07103435859025789	0.0	0.0	0.0	0.0
12	0.08469872446704735	0.0	0.0	0.0	0.0
13	0.09436210976452873	0.0	0.0	0.0	0.0
14	0.1061277051969008	0.0	0.0	0.0	0.0
15	0.11908003215703374	0.0	0.0	0.0	0.0
16	0.13542302062505487	0.0	0.0	0.0	0.0
17	0.14959598572802757	0.0	0.0	0.0	0.0
18	0.16268393914847604	0.0	0.0	0.0	0.0
19	0.17672127779113322	0.0	0.0	0.0	0.0
20	0.18977532459650281	0.0	0.0	0.0	0.0
21	0.20089669434237611	0.0	0.0	0.0	0.0
22	0.2128318228501426	0.0	0.0	0.0	0.0
23	0.2261910291912221	0.0	0.0	0.0	0.0
24	0.24239839119892773	0.0	0.0	0.0	0.0
25	0.2539944535559054	0.0	0.0	0.0	0.0
26	0.2704730684842421	0.0	0.0	0.0	0.0
27	0.28376446159516383	0.0	3.390661507888204E-5	0.0	0.0
28	0.3043796835631241	0.0	3.390661507888204E-5	0.0	0.0
29	0.316484345146285	0.0	3.390661507888204E-5	0.0	0.0
30	0.33174232193178194	0.0	3.390661507888204E-5	0.0	0.0
31	0.34540668780857137	0.0	3.390661507888204E-5	0.0	0.0
32	0.35968137275678075	0.0	3.390661507888204E-5	0.0	0.0
33	0.3716504078796261	0.0	3.390661507888204E-5	0.0	0.0
34	0.38433148191912797	0.0	3.390661507888204E-5	0.0	0.0
35	0.40345481282361745	0.0	3.390661507888204E-5	0.0	0.0
36	0.41664448608930255	0.0	3.390661507888204E-5	0.0	0.0
37	0.4314277702636951	0.0	3.390661507888204E-5	0.0	0.0
38	0.44309164585083055	0.0	3.390661507888204E-5	0.0	0.0
39	0.45485724128320265	0.0	3.390661507888204E-5	0.0	0.0
40	0.4667584631758902	0.0	3.390661507888204E-5	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATA	40	5.6170393E-9	46.0	13
TTGGGAT	32670	0.0	44.120293	5
GTTTGGG	300060	0.0	43.9718	3
GGTTTGG	306165	0.0	43.94764	2
TTTGGGA	107130	0.0	43.89601	4
TTTGGGG	156350	0.0	43.77576	4
GGGTTTG	308940	0.0	43.737522	1
TTGGGGC	35665	0.0	43.214077	5
TTGGGGG	52810	0.0	43.151676	5
TTGGGAC	19830	0.0	43.135147	5
TTGGGGT	27540	0.0	42.90995	5
TGGGATC	9970	0.0	42.885654	6
TTGGGAG	37270	0.0	42.636703	5
TGGGATT	11005	0.0	42.59337	6
TTGGGGA	43605	0.0	42.25502	5
TTTGGGC	23865	0.0	42.154617	4
TGGGGGG	22940	0.0	42.099827	6
TGGGACT	7595	0.0	41.851215	6
TGGGGCT	19430	0.0	41.51364	6
TGGGATG	8960	0.0	41.405136	6
>>END_MODULE