FastQCFastQC Report
Fri 17 Jun 2016
SRR1527636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318390
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA24930.7830019787053614Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15910.49970162379471716No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGA13820.4340588586324947No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11590.36401897044505166No Hit
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10830.34014887402242533No Hit
GAGATCGGGGTGGTTATCCAGTCTGTGTGCCAAAGTCAGCCATATCTCCC7080.22236879298972956No Hit
GAGATCGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAA7030.22079839190929365No Hit
GAGATCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG6750.21200414585885236No Hit
GAGATCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC6470.20320989980841106No Hit
GAGATCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG5930.1862495681397029No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA5850.18373692641100536No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5630.1768271616570872No Hit
GAGATCGGAGATGGAGAAGACACCATACTGAAGCTAAAAGTGATACCTGA5390.1692892364709947No Hit
GAGATCGGGGCTGGAAGCAGAGACTCCTGTGCGTCTCTCTCACGCTACCC5370.16866107603882033No Hit
GAGATCGGGGACTTCAAGTAGCCAAGCCGGCTTCGAGAGTCGTATCTATT4770.149816263073589No Hit
GAGATCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4310.13536857313357833Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGGAGGGGGAGGTGGGGTAAGTGCCTCCAGGAAGGCAGGCAGC4250.13348409183705517No Hit
GAGATCGGGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCT4150.1303432896761833No Hit
GAGATCGGGAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAG4050.1272024875153114No Hit
GAGATCGGGACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCGCTG3990.1253180062187883No Hit
GAGATCGGGCAGTCGGGCGTCTTGGCGCACGCCTTTAATCCCAGCATTCG3900.12249128427400359No Hit
GAGATCGGGAGCGTTCTTGTAATTTCACGCGCTTTTGTGATGGAGTGCTG3640.11432519865573668No Hit
GAGATCGGGTCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAG3380.10615911303746978No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA3370.10584503282138258No Hit
GAGATCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAA3360.1055309526052954No Hit
GAGATCGGGGTATTATATGTGGCTATATTTCCTAGAGCACCTGTGTTTTT3360.1055309526052954No Hit
GAGATCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT3250.10207607022833631No Hit
GAGATCGGGGCACGCAGGGCGGGGTCTCATTCTTGCGCTGGCCCCCAAGA3220.10113382958007475No Hit
GAGATCGGGGCACATACACACTATGAAGAGGTCTTCAGATAAACAACTTC3200.10050566914790036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG408.28004E-944.025
TCGCCTA207.847897E-444.032
ACAACCG207.847897E-444.025
TTAGGTT254.435568E-544.037
TTCTACG207.847897E-444.018
TTCTAAG302.5219306E-644.013
CGGGTCG302.5219306E-644.06
TCGTTGC207.847897E-444.037
TCTGACG254.435568E-544.027
GATACCG207.847897E-444.011
CAAACCG408.28004E-944.030
CGAACGG207.847897E-444.011
ACGCCTA254.435568E-544.032
TCCGCAT254.435568E-544.032
ATAGGTG254.435568E-544.010
TACTTGC207.847897E-444.030
CAAGTAA207.847897E-444.029
ATAGGCG207.847897E-444.032
CGCGGAC254.435568E-544.034
ACGTTCC207.847897E-444.024
Produced by FastQC (version 0.11.5)