##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	318390
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.514391155501116	33.0	31.0	34.0	31.0	34.0
2	32.28175508024749	33.0	31.0	34.0	31.0	34.0
3	32.79224221866264	34.0	31.0	34.0	31.0	34.0
4	36.21462985646534	37.0	37.0	37.0	35.0	37.0
5	36.31968654794434	37.0	37.0	37.0	35.0	37.0
6	36.38950343917837	37.0	37.0	37.0	35.0	37.0
7	36.59278557743648	37.0	37.0	37.0	35.0	37.0
8	36.698219165174784	37.0	37.0	37.0	35.0	37.0
9	38.640968623386414	39.0	39.0	39.0	39.0	39.0
10	38.22156474763655	39.0	39.0	39.0	37.0	39.0
11	37.982430352712086	39.0	38.0	39.0	35.0	39.0
12	37.76420427777254	39.0	37.0	39.0	35.0	39.0
13	37.688498382486884	39.0	38.0	39.0	35.0	39.0
14	38.55175413800685	40.0	38.0	40.0	35.0	40.0
15	38.52376330914915	40.0	38.0	40.0	35.0	40.0
16	38.544536574641164	40.0	38.0	40.0	35.0	40.0
17	38.567015295706526	40.0	38.0	40.0	35.0	40.0
18	38.426687395960926	40.0	38.0	40.0	35.0	40.0
19	38.40785200540218	40.0	38.0	40.0	35.0	40.0
20	38.338449700053395	40.0	38.0	40.0	35.0	40.0
21	38.40120606802977	40.0	38.0	40.0	35.0	40.0
22	38.38170796821508	40.0	38.0	40.0	35.0	40.0
23	38.294670058733	40.0	38.0	40.0	35.0	40.0
24	38.14748264706806	40.0	38.0	40.0	35.0	40.0
25	37.99266936775653	40.0	38.0	40.0	34.0	40.0
26	37.959232387951886	40.0	38.0	40.0	34.0	40.0
27	37.92064449260341	40.0	38.0	40.0	34.0	40.0
28	37.813844655925124	40.0	38.0	40.0	34.0	40.0
29	37.83400860579792	40.0	38.0	40.0	34.0	40.0
30	37.94402462388894	40.0	38.0	40.0	34.0	40.0
31	37.97558654480354	40.0	38.0	40.0	34.0	40.0
32	37.91885423537171	40.0	38.0	40.0	34.0	40.0
33	37.8474795062659	40.0	38.0	40.0	34.0	40.0
34	37.610201325418515	40.0	37.0	40.0	33.0	40.0
35	37.61425296020604	40.0	37.0	40.0	33.0	40.0
36	37.24765853198907	40.0	36.0	40.0	33.0	40.0
37	37.29901692892365	40.0	36.0	40.0	33.0	40.0
38	37.21762932252897	40.0	36.0	40.0	33.0	40.0
39	37.0729733974057	40.0	36.0	40.0	33.0	40.0
40	36.86985458085995	40.0	35.0	40.0	32.0	40.0
41	36.74910016018091	40.0	35.0	40.0	32.0	40.0
42	36.58468230786143	40.0	35.0	40.0	31.0	40.0
43	36.40226765916015	39.0	35.0	40.0	31.0	40.0
44	35.892283049090736	39.0	35.0	40.0	29.0	40.0
45	35.82322309117749	39.0	35.0	40.0	28.0	40.0
46	35.5195452118471	39.0	35.0	40.0	27.0	40.0
47	35.37151920600521	39.0	35.0	40.0	26.0	40.0
48	35.30522315399353	38.0	35.0	40.0	27.0	40.0
49	35.18981123779013	38.0	35.0	40.0	26.0	40.0
50	34.65365432331417	37.0	34.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	5.0
16	12.0
17	20.0
18	22.0
19	55.0
20	99.0
21	176.0
22	286.0
23	480.0
24	814.0
25	1047.0
26	1518.0
27	2174.0
28	2916.0
29	3575.0
30	4420.0
31	5242.0
32	6932.0
33	9509.0
34	15038.0
35	19282.0
36	26531.0
37	41108.0
38	106885.0
39	70241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.52385439241182	0.34768679920851786	0.06846948710700713	0.05998932127265304
8	98.87810546813655	0.9447532899902635	0.07569333207701247	0.10144790979616194
9	91.64420993121644	6.1876943371337045	1.0198184616351016	1.1482772700147617
10	40.389459467948114	40.681868149125286	9.693457709098904	9.235214673827695
11	31.725870787399103	23.724049122145797	26.311127862055965	18.238952228399132
12	27.221018248060552	23.077357957222276	25.69333207701247	24.0082917177047
13	27.483589308709444	20.957002418417662	27.255567071830143	24.303841201042747
14	25.365746411633534	24.229404189830085	25.94428216966613	24.460567228870254
15	25.388988347623982	23.506391532397373	29.84641477433336	21.25820534564528
16	27.48296114827727	27.14312635447093	23.495084644618235	21.878827852633563
17	30.58984264581174	23.116617984233173	24.194541285844405	22.098998084110683
18	23.964948647884672	24.290963912183173	28.12525519017557	23.61883224975659
19	26.025314865416625	27.02032098998084	25.372970256603537	21.581393887998995
20	23.93919407016552	26.33436979804642	27.178617418888784	22.547818712899275
21	29.997801438487386	23.19419579760671	26.400326643424734	20.40767612048117
22	25.16316467225729	24.675398096673888	23.715254876095354	26.44618235497346
23	23.621658971701372	27.098841044002636	25.114796318979867	24.16470366531612
24	26.44869499670216	27.42045918527592	23.146455604761456	22.98439021326047
25	24.47124595621722	26.969439994974714	26.290084487578124	22.26922956122994
26	25.83749489619649	26.41508841358083	23.60972392349006	24.137692766732624
27	22.319482395803888	27.91262288388454	26.166965042871947	23.60092967743962
28	23.526178586010868	27.1022959263796	26.97038223562298	22.401143251986557
29	26.157542636389337	24.915983542196678	23.56606677345394	25.36040704796005
30	24.699582273312604	25.575237915763687	28.101385093752945	21.623794717170767
31	28.909199409529197	26.74204591852759	22.041521404566726	22.30723326737649
32	24.984138949087598	27.911366563020195	24.08995257388737	23.014541914004838
33	26.143094946449324	26.068029774804486	27.194635509909233	20.59423976883696
34	22.148308678036372	26.584063569835735	29.672100254404977	21.59552749772292
35	27.796413203932286	27.566192405540374	24.323942334872324	20.313452055655016
36	25.40029523540312	29.393511102735637	23.930085743898992	21.276107917962246
37	24.209931216432675	31.66776594742297	22.67816200257546	21.44414083356889
38	23.879518829108953	31.759791450736518	23.133578315901882	21.227111404252646
39	22.76736078394422	30.057790759760046	25.257388737083453	21.91745971921229
40	23.760168346995822	30.434058858632497	24.694242909639122	21.111529884732562
41	22.336756807688683	30.601777694023053	25.5896856057037	21.471779892584568
42	22.14956499890072	30.628474512390465	23.957724802914665	23.26423568579415
43	23.360972392349005	28.915795094067025	25.185150287383394	22.53808222620057
44	23.39834793806338	30.48022865039731	23.7190238386884	22.402399572850907
45	22.807877131819467	30.36967241433462	23.991331386035995	22.83111906780992
46	24.03090549326298	27.508401645780335	24.27745846289142	24.183234398065263
47	22.72715851628506	27.917962247558027	25.23634536260561	24.118533873551304
48	22.98187757153177	27.861113728446245	25.239486164766483	23.917522535255504
49	23.079242438518797	28.875592826407864	25.71343321084205	22.331731524231287
50	23.96023744464336	29.776374886145923	24.085555450862152	22.177832218348566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	9.0
6	18.0
7	29.5
8	41.0
9	82.5
10	124.0
11	922.0
12	1720.0
13	1940.5
14	2161.0
15	2354.0
16	2547.0
17	1979.0
18	1411.0
19	1404.5
20	1398.0
21	1472.5
22	1547.0
23	1323.5
24	1100.0
25	1762.5
26	2425.0
27	2564.0
28	2703.0
29	3330.0
30	3957.0
31	4327.0
32	4697.0
33	5701.5
34	6706.0
35	7093.0
36	7480.0
37	9771.5
38	12063.0
39	12072.0
40	12081.0
41	12170.0
42	12259.0
43	13706.5
44	15154.0
45	15145.0
46	15136.0
47	16801.5
48	18467.0
49	18949.0
50	19431.0
51	18893.5
52	18356.0
53	20449.0
54	22542.0
55	21722.0
56	20902.0
57	21109.5
58	21317.0
59	21526.0
60	21735.0
61	21102.0
62	20469.0
63	18428.5
64	16388.0
65	13776.0
66	11164.0
67	9939.0
68	8714.0
69	6546.5
70	4379.0
71	3778.5
72	3178.0
73	2785.5
74	2393.0
75	1949.5
76	1506.0
77	950.5
78	395.0
79	281.5
80	168.0
81	134.5
82	101.0
83	73.5
84	46.0
85	26.5
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	318390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.04312321366877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39212097861683	9.442193536229153
2	8.664033904835291	2.2601212349634094
3	3.3880755153149678	1.3257325921040235
4	1.632633403968407	0.8517855460284557
5	0.8813330764785205	0.5747667954395552
6	0.5971874397996533	0.46735136153773676
7	0.49845887112309767	0.4551022331103364
8	0.34916201117318435	0.36433305066113886
9	0.31785783086110575	0.3731272967115801
>10	7.055480639568485	25.73133578315902
>50	2.834232325178193	25.45274663148968
>100	1.3557118089000193	28.537328433681964
>500	0.02167212483143903	1.743145199283897
>1k	0.01204006935079946	2.42093030560005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	2493	0.7830019787053614	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1591	0.49970162379471716	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGA	1382	0.4340588586324947	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1159	0.36401897044505166	No Hit
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1083	0.34014887402242533	No Hit
GAGATCGGGGTGGTTATCCAGTCTGTGTGCCAAAGTCAGCCATATCTCCC	708	0.22236879298972956	No Hit
GAGATCGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAA	703	0.22079839190929365	No Hit
GAGATCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG	675	0.21200414585885236	No Hit
GAGATCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC	647	0.20320989980841106	No Hit
GAGATCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	593	0.1862495681397029	No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	585	0.18373692641100536	No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	563	0.1768271616570872	No Hit
GAGATCGGAGATGGAGAAGACACCATACTGAAGCTAAAAGTGATACCTGA	539	0.1692892364709947	No Hit
GAGATCGGGGCTGGAAGCAGAGACTCCTGTGCGTCTCTCTCACGCTACCC	537	0.16866107603882033	No Hit
GAGATCGGGGACTTCAAGTAGCCAAGCCGGCTTCGAGAGTCGTATCTATT	477	0.149816263073589	No Hit
GAGATCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	431	0.13536857313357833	Illumina Single End Adapter 2 (97% over 34bp)
GAGATCGGGGAGGGGGAGGTGGGGTAAGTGCCTCCAGGAAGGCAGGCAGC	425	0.13348409183705517	No Hit
GAGATCGGGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCT	415	0.1303432896761833	No Hit
GAGATCGGGAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAG	405	0.1272024875153114	No Hit
GAGATCGGGACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCGCTG	399	0.1253180062187883	No Hit
GAGATCGGGCAGTCGGGCGTCTTGGCGCACGCCTTTAATCCCAGCATTCG	390	0.12249128427400359	No Hit
GAGATCGGGAGCGTTCTTGTAATTTCACGCGCTTTTGTGATGGAGTGCTG	364	0.11432519865573668	No Hit
GAGATCGGGTCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAG	338	0.10615911303746978	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA	337	0.10584503282138258	No Hit
GAGATCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAA	336	0.1055309526052954	No Hit
GAGATCGGGGTATTATATGTGGCTATATTTCCTAGAGCACCTGTGTTTTT	336	0.1055309526052954	No Hit
GAGATCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	325	0.10207607022833631	No Hit
GAGATCGGGGCACGCAGGGCGGGGTCTCATTCTTGCGCTGGCCCCCAAGA	322	0.10113382958007475	No Hit
GAGATCGGGGCACATACACACTATGAAGAGGTCTTCAGATAAACAACTTC	320	0.10050566914790036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.0940042086748956	0.0	0.0	0.0	0.0
3	3.0940042086748956	0.0	0.0	0.0	0.0
4	3.0940042086748956	0.0	0.0	0.0	0.0
5	3.0940042086748956	0.0	0.0	0.0	0.0
6	3.0940042086748956	0.0	0.0	0.0	0.0
7	3.1156757435849114	0.0	0.0	0.0	0.0
8	3.147711925625805	0.0	0.0	0.0	0.0
9	3.1621596155658156	0.0	0.0	0.0	0.0
10	3.1948239580388833	0.0	0.0	0.0	0.0
11	3.231257263104997	0.0	0.0	0.0	0.0
12	3.2532428782311	0.0	0.0	0.0	0.0
13	3.2915606645937374	0.0	0.0	0.0	0.0
14	3.3882973711485915	0.0	0.0	0.0	0.0
15	3.4234743553503564	0.0	0.0	0.0	0.0
16	3.4822073557586606	0.0	0.0	0.0	0.0
17	3.514243537799554	0.0	0.0	0.0	0.0
18	3.5550739658908888	0.0	0.0	0.0	0.0
19	3.58396934577091	0.0	0.0	0.0	0.0
20	3.631395458400075	0.0	0.0	0.0	0.0
21	3.686359496215333	0.0	0.0	0.0	0.0
22	3.737554571437545	0.0	0.0	0.0	0.0
23	3.808850780489337	0.0	0.0	0.0	0.0
24	3.8581613744150256	0.0	0.0	0.0	0.0
25	3.9081001287728885	0.0	0.0	0.0	0.0
26	3.96714720939728	0.0	0.0	0.0	0.0
27	4.009862118785137	0.0	0.0	0.0	0.0
28	4.0824146487012785	0.0	0.0	0.0	0.0
29	4.127014039385659	0.0	0.0	0.0	0.0
30	4.173497911366563	0.0	0.0	0.0	0.0
31	4.210873457080939	0.0	0.0	0.0	0.0
32	4.242909639121832	0.0	0.0	0.0	0.0
33	4.267721976192719	0.0	0.0	0.0	0.0
34	4.309180564716229	0.0	0.0	0.0	0.0
35	4.360061559722353	0.0	0.0	0.0	0.0
36	4.393039982411508	0.0	0.0	0.0	0.0
37	4.424762084236314	0.0	0.0	0.0	0.0
38	4.44863218065894	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	40	8.28004E-9	44.0	25
TCGCCTA	20	7.847897E-4	44.0	32
ACAACCG	20	7.847897E-4	44.0	25
TTAGGTT	25	4.435568E-5	44.0	37
TTCTACG	20	7.847897E-4	44.0	18
TTCTAAG	30	2.5219306E-6	44.0	13
CGGGTCG	30	2.5219306E-6	44.0	6
TCGTTGC	20	7.847897E-4	44.0	37
TCTGACG	25	4.435568E-5	44.0	27
GATACCG	20	7.847897E-4	44.0	11
CAAACCG	40	8.28004E-9	44.0	30
CGAACGG	20	7.847897E-4	44.0	11
ACGCCTA	25	4.435568E-5	44.0	32
TCCGCAT	25	4.435568E-5	44.0	32
ATAGGTG	25	4.435568E-5	44.0	10
TACTTGC	20	7.847897E-4	44.0	30
CAAGTAA	20	7.847897E-4	44.0	29
ATAGGCG	20	7.847897E-4	44.0	32
CGCGGAC	25	4.435568E-5	44.0	34
ACGTTCC	20	7.847897E-4	44.0	24
>>END_MODULE