FastQCFastQC Report
Fri 17 Jun 2016
SRR1527635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473406
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20800.43936916726868686No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG18450.38972890077438815No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16150.3411448101629468No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA15610.32973811062808667Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14500.30629100602865195No Hit
GAGATCGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAAAT11640.2458777455292075No Hit
GAGATCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC10750.22707781481434539No Hit
GAGATCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA9960.21039023586519817No Hit
GAGATCGGGGTGGTTATCCAGTCTGTGTGCCAAAGTCAGCCATATCTCCCAC9940.20996776551205518No Hit
GAGATCGGGGCTGGAAGCAGAGACTCCTGTGCGTCTCTCTCACGCTACCCCT8970.18947795338462123No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG8410.1776487834966181No Hit
GAGATCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG8270.17469149102461734No Hit
GAGATCGGAGATGGAGAAGACACCATACTGAAGCTAAAAGTGATACCTGAAA8020.16941061161033025No Hit
GAGATCGGGGACTTCAAGTAGCCAAGCCGGCTTCGAGAGTCGTATCTATTGT7980.1685656709040443No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7150.15103315124861114No Hit
GAGATCGGGAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAGAC6510.13751409994803615No Hit
GAGATCGGGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTG6180.13054333912117716No Hit
GAGATCGGGACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCGCTGCC6020.12716357629603345No Hit
GAGATCGGGCAGTCGGGCGTCTTGGCGCACGCCTTTAATCCCAGCATTCGGG5770.12188269688174633No Hit
GAGATCGGGGAGGGGGAGGTGGGGTAAGTGCCTCCAGGAAGGCAGGCAGCCT5750.12146022652860336No Hit
GAGATCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG5410.1142782305251729No Hit
GAGATCGGGAGCGTTCTTGTAATTTCACGCGCTTTTGTGATGGAGTGCTGTT5350.113010819465744No Hit
GAGATCGGGTCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTA5350.113010819465744No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG5210.11005352699374321No Hit
GAGATCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT5010.10582882346231352No Hit
GAGATCGGGGTATTATATGTGGCTATATTTCCTAGAGCACCTGTGTTTTTCT4980.10519511793259909No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA4940.10435017722631314No Hit
GAGATCGGGGCACGCAGGGCGGGGTCTCATTCTTGCGCTGGCCCCCAAGAGC4830.10202659028402683No Hit
GAGATCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA4810.10160411993088386No Hit
GAGATCGGGGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTT4780.1009704144011694No Hit
GAGATCGGTAGTGCAGTGCTGAGATCGGGGTGCTGACCTCCAAGTACCGTTA4770.1007591792245979No Hit
GAGATCGAGGATCGGGTGGTTCTTGGTGGAGATCATTATTTTAGATTTAATC4760.10054794404802643No Hit
GAGATCGGGGCACATACACACTATGAAGAGGTCTTCAGATAAACAACTTCCA4750.10033670887145493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCC351.0178701E-746.00000427
CGCGCAA351.0178701E-746.00000410
GCGCCGT351.0178701E-746.00000417
TAACGCG750.046.00000434
CTGACGC351.0178701E-746.00000434
ACCCTCG351.0178701E-746.00000426
ATCGTCC351.0178701E-746.00000432
CGGATTG351.0178701E-746.0000046
ACGTGTT351.0178701E-746.00000443
TGTCGCA351.0178701E-746.00000446
TAGTAAC351.0178701E-746.00000426
TCACGCG253.4140896E-546.025
TATTTCG206.307676E-446.029
ATTTACG253.4140896E-546.046
ATTTACC253.4140896E-546.022
GTTGACG253.4140896E-546.016
GTTGACC206.307676E-446.041
ACAACCG206.307676E-446.025
TTTAGCG253.4140896E-546.042
AACGTCG405.5988494E-946.023
Produced by FastQC (version 0.11.5)