##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473406
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13314364414477	33.0	31.0	34.0	31.0	34.0
2	31.914445528785016	31.0	31.0	34.0	30.0	34.0
3	32.490494417054286	34.0	31.0	34.0	31.0	34.0
4	36.03916933879165	37.0	35.0	37.0	35.0	37.0
5	35.98745474286342	37.0	35.0	37.0	35.0	37.0
6	36.13052855265882	37.0	37.0	37.0	35.0	37.0
7	36.500948445942804	37.0	37.0	37.0	35.0	37.0
8	36.630055385863294	37.0	37.0	37.0	35.0	37.0
9	38.58039399585134	39.0	39.0	39.0	37.0	39.0
10	37.98313498350253	39.0	38.0	39.0	35.0	39.0
11	37.62392534103919	39.0	37.0	39.0	35.0	39.0
12	37.45230098477839	39.0	37.0	39.0	35.0	39.0
13	37.379678331073116	39.0	37.0	39.0	35.0	39.0
14	38.25128325369767	40.0	38.0	40.0	35.0	40.0
15	38.25689154763565	40.0	38.0	40.0	35.0	40.0
16	38.261249329328315	40.0	38.0	40.0	35.0	40.0
17	38.26511070835604	40.0	38.0	40.0	34.0	40.0
18	38.192834480340345	40.0	38.0	40.0	34.0	40.0
19	38.169442296886814	40.0	38.0	40.0	34.0	40.0
20	38.17210386011162	40.0	38.0	40.0	34.0	40.0
21	38.196324085457306	40.0	38.0	40.0	34.0	40.0
22	38.16065702589321	40.0	38.0	40.0	34.0	40.0
23	38.07617985407874	40.0	38.0	40.0	34.0	40.0
24	37.96344786504607	40.0	38.0	40.0	34.0	40.0
25	37.828155959155566	40.0	37.0	40.0	34.0	40.0
26	37.70600076889604	40.0	37.0	40.0	33.0	40.0
27	37.689477953384625	40.0	37.0	40.0	34.0	40.0
28	37.62208337029949	40.0	37.0	40.0	33.0	40.0
29	37.48928826419606	40.0	37.0	40.0	33.0	40.0
30	37.36793365525574	40.0	36.0	40.0	33.0	40.0
31	37.277155338124146	40.0	36.0	40.0	33.0	40.0
32	37.12730510386434	39.0	36.0	40.0	32.0	40.0
33	37.04059940093704	39.0	36.0	40.0	32.0	40.0
34	36.958112064485874	39.0	36.0	40.0	32.0	40.0
35	36.88009446437096	39.0	35.0	40.0	32.0	40.0
36	36.51360565772297	39.0	35.0	40.0	31.0	40.0
37	36.53963616853187	39.0	35.0	40.0	31.0	40.0
38	36.458992915172175	39.0	35.0	40.0	31.0	40.0
39	36.31981005732923	39.0	35.0	40.0	30.0	40.0
40	36.1066737641686	39.0	35.0	40.0	30.0	40.0
41	35.976322649058105	38.0	35.0	40.0	30.0	40.0
42	35.84591450044993	38.0	35.0	40.0	30.0	40.0
43	35.60784823175034	38.0	35.0	40.0	29.0	40.0
44	35.29550111320938	38.0	35.0	40.0	27.0	40.0
45	35.13417447180644	38.0	34.0	40.0	26.0	40.0
46	34.88199980566364	38.0	34.0	40.0	26.0	40.0
47	34.68363730075242	37.0	34.0	40.0	25.0	40.0
48	34.56530124248531	37.0	34.0	40.0	24.0	40.0
49	34.79977017612789	37.0	34.0	40.0	26.0	40.0
50	34.7281403277525	37.0	34.0	40.0	26.0	40.0
51	34.693438612945336	37.0	34.0	40.0	26.0	40.0
52	34.24183681660139	37.0	34.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	3.0
16	10.0
17	28.0
18	58.0
19	109.0
20	237.0
21	401.0
22	682.0
23	1141.0
24	1686.0
25	2359.0
26	3327.0
27	4486.0
28	5577.0
29	7002.0
30	8355.0
31	10748.0
32	13893.0
33	17833.0
34	26311.0
35	31196.0
36	47188.0
37	70430.0
38	155630.0
39	64714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.56105330308446	0.3470593951069484	0.04626050366915502	0.045626798139440566
8	98.90474560947685	0.937672948800818	0.06358178814801672	0.09399965357431042
9	91.93018254943959	5.813614529600385	1.0614567622717075	1.1947461586883141
10	40.28529422947745	40.71811510627242	9.808916659273436	9.187674004976701
11	31.25900389940136	23.70966992391309	26.47685073699953	18.55447543968602
12	27.278488232088314	22.491476660625338	26.020160285251983	24.209874822034365
13	27.239832194775733	20.861374803023196	27.438182025576356	24.460610976624718
14	25.472216237225553	24.163403083188637	26.113314998120007	24.251065681465803
15	25.627051621652452	23.342965657384994	29.64347726898265	21.386505451979907
16	27.57569612552439	26.973464637119086	23.853309843981698	21.59752939337482
17	30.354072402969123	23.248121063104396	24.198045652146362	22.19976088178012
18	24.048068676780606	24.073628133145757	27.92000946333591	23.958293726737725
19	25.836808151987935	26.70308361110759	25.634867323185595	21.82524091371888
20	24.229942163808655	26.122609345889153	26.999869034190528	22.647579456111664
21	29.68910406712209	23.0918070324415	26.72547453982417	20.493614360612245
22	25.36617617858667	24.473707557572148	23.834298678090267	26.325817585750922
23	23.70396657414566	27.056480061511685	25.345686366459237	23.89386699788342
24	26.130425047422296	27.173715584508855	23.49167522169132	23.20418414637753
25	24.90568349366083	26.658301753674436	26.080362310574856	22.355652442089877
26	26.094726302581716	26.365318563769787	23.623908442225066	23.91604669142343
27	22.640186224931664	27.62998356590326	25.955944791574254	23.77388541759082
28	23.70544522038166	27.08605298623169	26.549726872916697	22.658774920469956
29	26.2096382386366	24.92511712990541	23.71368339226795	25.15156123919004
30	24.880335272472255	25.442220842152402	27.984436192190216	21.69300769318513
31	28.84078359801101	26.488046201357818	22.21687093108241	22.45429926954876
32	25.157264588957467	27.609704988952398	24.396395482946982	22.836634939143146
33	26.49966413606925	25.884336066716518	26.790534974208185	20.825464823006044
34	22.180538480712116	26.418549828265803	29.572713484831205	21.82819820619088
35	27.738769681837578	27.412833804387777	24.32626540432525	20.522131109449393
36	25.572764181273577	28.824518489415006	24.22656240098351	21.376154928327903
37	24.477721025927003	31.2564690772825	22.786572202295705	21.47923769449479
38	24.034972095833172	31.21232092537906	23.31275902713527	21.439947951652492
39	23.069204868548347	29.353451371549998	25.44179837179926	22.13554538810239
40	23.810429103137686	29.907310004520433	24.90082508459969	21.381435807742193
41	22.44859591978133	30.03700840293532	25.822021689627935	21.692373987655415
42	22.329459280195014	30.031305053167895	24.15643232236178	23.482803344275315
43	23.628133145756497	28.51294660397207	25.292455101963217	22.566465148308218
44	23.38500145752272	29.944487395597015	23.954069023206294	22.71644212367397
45	22.981542270271184	29.67811983794037	24.259726323705234	23.08061156808321
46	23.96906672074287	27.18132005086543	24.449204277089855	24.40040895130184
47	23.06329028360435	27.36826318213119	25.319281969387795	24.24916456487666
48	23.325644372906133	27.33657790564547	25.44327701803526	23.894500703413136
49	23.420066496833584	28.391275142266892	25.878421481772516	22.310236879127007
50	23.979206009218306	28.959497767244187	24.5022243064093	22.559071917128215
51	22.012184044984643	28.522874657270926	25.996501945475973	23.468439352268454
52	23.068782398195207	28.600820437425806	25.249151890766065	23.081245273612925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	13.0
6	26.0
7	29.0
8	32.0
9	87.5
10	143.0
11	1281.5
12	2420.0
13	2613.5
14	3142.0
15	3477.0
16	2623.0
17	1769.0
18	1664.5
19	1560.0
20	1799.0
21	2038.0
22	1857.0
23	1676.0
24	2061.0
25	2446.0
26	2998.5
27	3551.0
28	3939.0
29	4327.0
30	5532.5
31	6738.0
32	7858.0
33	8978.0
34	9426.0
35	9874.0
36	11539.5
37	13205.0
38	14456.0
39	16595.0
40	17483.0
41	17879.0
42	18275.0
43	20533.5
44	22792.0
45	23865.0
46	24938.0
47	24435.0
48	23932.0
49	24599.5
50	25267.0
51	26823.0
52	28379.0
53	29195.5
54	30012.0
55	30821.5
56	31631.0
57	31574.0
58	31517.0
59	30930.5
60	30344.0
61	30392.5
62	30441.0
63	26634.5
64	21156.0
65	19484.0
66	15993.5
67	12503.0
68	11244.0
69	9985.0
70	7402.0
71	4819.0
72	4174.0
73	3529.0
74	3054.5
75	2580.0
76	1925.0
77	1270.0
78	784.5
79	299.0
80	265.5
81	232.0
82	152.5
83	73.0
84	42.0
85	11.0
86	8.0
87	5.0
88	3.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	473406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.819664305057392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39469216334554	8.675006231437708
2	8.980430703243677	2.122913524543415
3	3.772674470556697	1.3377523732272087
4	1.8872308104727014	0.8922573858379489
5	1.0687159324457154	0.6315931779487374
6	0.6862657492628005	0.4866858468206994
7	0.4753820033955857	0.3933198987761034
8	0.34134572424269505	0.32276734980122773
9	0.29309266374765436	0.3117831206195105
>10	4.09614869091234	13.304225126001784
>50	2.98811544991511	25.304917977380935
>100	1.971226878741846	41.291618610664
>500	0.03216870699669377	2.6459318217344094
>1k	0.012510052720936466	2.2792275552063135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2080	0.43936916726868686	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG	1845	0.38972890077438815	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1615	0.3411448101629468	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	1561	0.32973811062808667	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1450	0.30629100602865195	No Hit
GAGATCGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAAAT	1164	0.2458777455292075	No Hit
GAGATCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	1075	0.22707781481434539	No Hit
GAGATCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	996	0.21039023586519817	No Hit
GAGATCGGGGTGGTTATCCAGTCTGTGTGCCAAAGTCAGCCATATCTCCCAC	994	0.20996776551205518	No Hit
GAGATCGGGGCTGGAAGCAGAGACTCCTGTGCGTCTCTCTCACGCTACCCCT	897	0.18947795338462123	No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	841	0.1776487834966181	No Hit
GAGATCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	827	0.17469149102461734	No Hit
GAGATCGGAGATGGAGAAGACACCATACTGAAGCTAAAAGTGATACCTGAAA	802	0.16941061161033025	No Hit
GAGATCGGGGACTTCAAGTAGCCAAGCCGGCTTCGAGAGTCGTATCTATTGT	798	0.1685656709040443	No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	715	0.15103315124861114	No Hit
GAGATCGGGAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAGAC	651	0.13751409994803615	No Hit
GAGATCGGGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTG	618	0.13054333912117716	No Hit
GAGATCGGGACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCGCTGCC	602	0.12716357629603345	No Hit
GAGATCGGGCAGTCGGGCGTCTTGGCGCACGCCTTTAATCCCAGCATTCGGG	577	0.12188269688174633	No Hit
GAGATCGGGGAGGGGGAGGTGGGGTAAGTGCCTCCAGGAAGGCAGGCAGCCT	575	0.12146022652860336	No Hit
GAGATCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG	541	0.1142782305251729	No Hit
GAGATCGGGAGCGTTCTTGTAATTTCACGCGCTTTTGTGATGGAGTGCTGTT	535	0.113010819465744	No Hit
GAGATCGGGTCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTA	535	0.113010819465744	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG	521	0.11005352699374321	No Hit
GAGATCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	501	0.10582882346231352	No Hit
GAGATCGGGGTATTATATGTGGCTATATTTCCTAGAGCACCTGTGTTTTTCT	498	0.10519511793259909	No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	494	0.10435017722631314	No Hit
GAGATCGGGGCACGCAGGGCGGGGTCTCATTCTTGCGCTGGCCCCCAAGAGC	483	0.10202659028402683	No Hit
GAGATCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA	481	0.10160411993088386	No Hit
GAGATCGGGGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTT	478	0.1009704144011694	No Hit
GAGATCGGTAGTGCAGTGCTGAGATCGGGGTGCTGACCTCCAAGTACCGTTA	477	0.1007591792245979	No Hit
GAGATCGAGGATCGGGTGGTTCTTGGTGGAGATCATTATTTTAGATTTAATC	476	0.10054794404802643	No Hit
GAGATCGGGGCACATACACACTATGAAGAGGTCTTCAGATAAACAACTTCCA	475	0.10033670887145493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.752605585903009	0.0	0.0	0.0	0.0
3	2.752605585903009	0.0	0.0	0.0	0.0
4	2.752605585903009	0.0	0.0	0.0	0.0
5	2.752605585903009	0.0	0.0	0.0	0.0
6	2.752605585903009	0.0	0.0	0.0	0.0
7	2.7692931648521566	0.0	0.0	0.0	0.0
8	2.798021148865878	0.0	0.0	0.0	0.0
9	2.8147087278150256	0.0	0.0	0.0	0.0
10	2.843225476652176	0.0	0.0	0.0	0.0
11	2.8746995179613273	0.0	0.0	0.0	0.0
12	2.8932882134996176	0.0	0.0	0.0	0.0
13	2.927508312104198	0.0	0.0	0.0	0.0
14	3.0014406239042177	0.0	0.0	0.0	0.0
15	3.024254022973938	0.0	0.0	0.0	0.0
16	3.067557234171092	0.0	0.0	0.0	0.0
17	3.0962852181848137	0.0	0.0	0.0	0.0
18	3.1321951982019662	0.0	0.0	0.0	0.0
19	3.1659928264534036	0.0	0.0	0.0	0.0
20	3.2057050396488425	0.0	0.0	0.0	0.0
21	3.253444189553998	0.0	0.0	0.0	0.0
22	3.2984372821637242	0.0	0.0	0.0	0.0
23	3.357371896427168	0.0	0.0	0.0	0.0
24	3.402576224213466	0.0	0.0	0.0	0.0
25	3.4477805519997635	0.0	0.0	0.0	0.0
26	3.491717468726632	0.0	0.0	0.0	0.0
27	3.5280499190969272	0.0	0.0	0.0	0.0
28	3.5907867665386584	0.0	0.0	0.0	0.0
29	3.6319776259700975	0.0	0.0	0.0	0.0
30	3.6725347798718224	0.0	0.0	0.0	0.0
31	3.6999953528261154	0.0	0.0	0.0	0.0
32	3.732525570018124	0.0	0.0	0.0	0.0
33	3.7612535540318457	0.0	0.0	0.0	0.0
34	3.795051182283283	0.0	0.0	0.0	0.0
35	3.8398330397164377	0.0	0.0	0.0	0.0
36	3.868772258906731	0.0	0.0	0.0	0.0
37	3.9000350650393107	0.0	0.0	0.0	0.0
38	3.9268619324638894	0.0	0.0	0.0	0.0
39	3.9492528611804665	0.0	0.0	0.0	0.0
40	3.9710100843673297	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCC	35	1.0178701E-7	46.000004	27
CGCGCAA	35	1.0178701E-7	46.000004	10
GCGCCGT	35	1.0178701E-7	46.000004	17
TAACGCG	75	0.0	46.000004	34
CTGACGC	35	1.0178701E-7	46.000004	34
ACCCTCG	35	1.0178701E-7	46.000004	26
ATCGTCC	35	1.0178701E-7	46.000004	32
CGGATTG	35	1.0178701E-7	46.000004	6
ACGTGTT	35	1.0178701E-7	46.000004	43
TGTCGCA	35	1.0178701E-7	46.000004	46
TAGTAAC	35	1.0178701E-7	46.000004	26
TCACGCG	25	3.4140896E-5	46.0	25
TATTTCG	20	6.307676E-4	46.0	29
ATTTACG	25	3.4140896E-5	46.0	46
ATTTACC	25	3.4140896E-5	46.0	22
GTTGACG	25	3.4140896E-5	46.0	16
GTTGACC	20	6.307676E-4	46.0	41
ACAACCG	20	6.307676E-4	46.0	25
TTTAGCG	25	3.4140896E-5	46.0	42
AACGTCG	40	5.5988494E-9	46.0	23
>>END_MODULE