FastQCFastQC Report
Fri 17 Jun 2016
SRR1527634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476055
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20000.42011952400457925No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG18640.3915513963722679No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14510.3047967146653223No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA14480.30416653537931543Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13140.2760185272710086No Hit
GAGATCGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAAAT11350.23841782987259874No Hit
GAGATCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC10580.22224322819842246No Hit
GAGATCGGGGTGGTTATCCAGTCTGTGTGCCAAAGTCAGCCATATCTCCCAC9540.2003970129501843No Hit
GAGATCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA9460.19871653485416604No Hit
GAGATCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG8760.18401235151400572No Hit
GAGATCGGGGCTGGAAGCAGAGACTCCTGTGCGTCTCTCTCACGCTACCCCT8460.17771055865393703No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG7920.1663673315058134No Hit
GAGATCGGAGATGGAGAAGACACCATACTGAAGCTAAAAGTGATACCTGAAA7760.16300637531377676No Hit
GAGATCGGGGACTTCAAGTAGCCAAGCCGGCTTCGAGAGTCGTATCTATTGT7650.16069571793175158No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC6580.1382193233975066No Hit
GAGATCGGGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTG6250.13128735125143104No Hit
GAGATCGGGAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAGAC6040.12687609624938295No Hit
GAGATCGGGGAGGGGGAGGTGGGGTAAGTGCCTCCAGGAAGGCAGGCAGCCT5930.12456543886735776No Hit
GAGATCGGGACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCGCTGCC5840.12267490100933715No Hit
GAGATCGGGAGCGTTCTTGTAATTTCACGCGCTTTTGTGATGGAGTGCTGTT5400.11343227148123641No Hit
GAGATCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG5380.11301215195723183No Hit
GAGATCGGGCAGTCGGGCGTCTTGGCGCACGCCTTTAATCCCAGCATTCGGG5300.11133167386121352No Hit
GAGATCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT5040.10587012004915398No Hit
GAGATCGGGTCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTA5010.10523994076314712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG405.5988494E-946.025
CAGGTCG206.307712E-446.020
AACGTCT253.414119E-546.017
CGGGTCG253.414119E-546.06
CCTTATT206.307712E-446.045
TCGTTGC253.414119E-546.037
TCGTTAG551.8189894E-1246.028
ACCGGTA253.414119E-546.024
CGAACCC206.307712E-446.045
ACGCCTA206.307712E-446.032
CGTATTT206.307712E-446.013
CGTATTG301.859089E-646.013
AGTCATA253.414119E-546.042
CTCGTCA253.414119E-546.028
GTACACC206.307712E-446.021
GATCGAC206.307712E-446.028
ATAGGCG253.414119E-546.032
GTTTCGC206.307712E-446.017
CGAAAGG301.859089E-646.023
ACGTTGA253.414119E-546.041
Produced by FastQC (version 0.11.5)