##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476055
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.080360462551596	33.0	31.0	34.0	31.0	34.0
2	31.87331295753642	31.0	31.0	34.0	30.0	34.0
3	32.46351577023663	34.0	31.0	34.0	31.0	34.0
4	36.01554442238817	37.0	35.0	37.0	35.0	37.0
5	36.00902416737562	37.0	35.0	37.0	35.0	37.0
6	36.075982817111466	37.0	37.0	37.0	35.0	37.0
7	36.470214576046885	37.0	37.0	37.0	35.0	37.0
8	36.607807921353626	37.0	37.0	37.0	35.0	37.0
9	38.53523227358183	39.0	39.0	39.0	37.0	39.0
10	37.905733581203854	39.0	38.0	39.0	35.0	39.0
11	37.591458970076985	39.0	37.0	39.0	35.0	39.0
12	37.38864626986378	39.0	37.0	39.0	35.0	39.0
13	37.18535043219796	39.0	37.0	39.0	34.0	39.0
14	38.18972177584523	40.0	38.0	40.0	34.0	40.0
15	38.2125258636082	40.0	38.0	40.0	34.0	40.0
16	38.24011931394482	40.0	38.0	40.0	34.0	40.0
17	38.27747004022645	40.0	38.0	40.0	34.0	40.0
18	38.21639306382666	40.0	38.0	40.0	34.0	40.0
19	38.150839713898606	40.0	38.0	40.0	34.0	40.0
20	38.17361229269727	40.0	38.0	40.0	34.0	40.0
21	38.2588650471059	40.0	38.0	40.0	34.0	40.0
22	38.114001533436266	40.0	38.0	40.0	34.0	40.0
23	38.05243931898625	40.0	38.0	40.0	34.0	40.0
24	37.98832277783029	40.0	38.0	40.0	34.0	40.0
25	37.81775215048681	40.0	37.0	40.0	34.0	40.0
26	37.64982827614456	40.0	37.0	40.0	33.0	40.0
27	37.64868134984403	40.0	37.0	40.0	33.0	40.0
28	37.5930029093277	40.0	37.0	40.0	33.0	40.0
29	37.45637583892618	40.0	37.0	40.0	33.0	40.0
30	37.32219806534959	40.0	36.0	40.0	33.0	40.0
31	37.25722448036466	40.0	36.0	40.0	33.0	40.0
32	37.10158280030669	39.0	36.0	40.0	32.0	40.0
33	36.84308745838191	39.0	35.0	40.0	31.0	40.0
34	36.868502588986566	39.0	35.0	40.0	31.0	40.0
35	36.86327420151033	39.0	35.0	40.0	32.0	40.0
36	36.36891955761414	39.0	35.0	40.0	30.0	40.0
37	36.45802480805789	39.0	35.0	40.0	31.0	40.0
38	36.1049626618773	39.0	35.0	40.0	30.0	40.0
39	36.137826511642565	39.0	35.0	40.0	30.0	40.0
40	35.99921017529487	38.0	35.0	40.0	30.0	40.0
41	35.67704361890958	38.0	35.0	40.0	29.0	40.0
42	35.6976189725977	38.0	35.0	40.0	29.0	40.0
43	35.58041612838853	38.0	35.0	40.0	29.0	40.0
44	35.258152944512716	38.0	35.0	40.0	27.0	40.0
45	35.06303683397927	38.0	34.0	40.0	26.0	40.0
46	34.65830838873659	38.0	34.0	40.0	24.0	40.0
47	34.38495341924778	37.0	34.0	40.0	23.0	40.0
48	34.409633340685424	37.0	34.0	40.0	24.0	40.0
49	34.35113379756541	37.0	34.0	40.0	24.0	40.0
50	34.08141706315447	37.0	33.0	40.0	23.0	40.0
51	34.330814716786925	37.0	34.0	40.0	24.0	40.0
52	34.059875434561135	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	4.0
17	26.0
18	52.0
19	103.0
20	206.0
21	442.0
22	723.0
23	1126.0
24	1693.0
25	2477.0
26	3500.0
27	4760.0
28	5895.0
29	7263.0
30	8592.0
31	11100.0
32	14556.0
33	18664.0
34	27285.0
35	32713.0
36	49554.0
37	76479.0
38	153543.0
39	55297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.55236264717313	0.3484891451617985	0.05104452216655638	0.04810368549852433
8	98.92512419783428	0.911659367089937	0.07037002027076704	0.09284641480501203
9	91.86900673241537	5.862137778197897	1.064582873827604	1.2042726155591266
10	40.38314900589218	40.62009641743076	9.868607618867568	9.128146957809497
11	31.45203810484083	23.643696631691714	26.520885191837078	18.383380071630377
12	27.36910651080232	22.438793836846582	25.961916165148985	24.23018348720211
13	27.48547961895159	20.766718131308355	27.26533698837319	24.48246526136686
14	25.498944449695937	24.10477780928674	26.172816166199286	24.223461574818035
15	25.72223797670437	23.34394135131445	29.436934807952863	21.496885864028318
16	27.583157408282656	26.878406906764972	23.86026824631608	21.678167438636294
17	30.546260411086955	22.98999065234059	24.151831195975255	22.311917740597202
18	24.047851613784122	24.057514362836226	27.96672653369884	23.927907489680813
19	25.837350726281628	26.681160790244824	25.61615779689322	21.865330686580332
20	24.160863765741354	26.17974813834536	26.97986577181208	22.679522324101207
21	29.799708016930815	23.05783995546733	26.681370850006825	20.461081177595027
22	25.43865729800128	24.419027213242167	23.83968238963985	26.3026330991167
23	23.75124722983689	27.000451628488303	25.297707197697743	23.95059394397706
24	26.00749913350348	27.229836888594804	23.55505141212675	23.207612565774962
25	24.783901019840144	26.49189694468076	26.258310489334214	22.465891546144878
26	26.119671046412705	26.083120647824305	23.767421831511065	24.029786474251924
27	22.60663158668641	27.57244436042054	25.861297539149884	23.95962651374316
28	23.796200018905378	27.070821648759075	26.612471248070076	22.520507084265475
29	26.331411286511013	24.813519446282466	23.571015954038923	25.284053313167597
30	24.900484187751417	25.44306855300333	27.9083299198622	21.748117339383054
31	28.95316717606159	26.428879016080074	22.197645230067955	22.420308577790383
32	25.263887576015374	27.630210794971173	24.2474083876863	22.858493241327157
33	26.635577821890326	25.762569451008808	26.745649137179527	20.856203589921332
34	22.338385270609486	26.312715967692807	29.471174549159233	21.877724212538467
35	27.749944859312475	27.439266471311086	24.339834682967304	20.470953986409132
36	26.01737194231759	28.630935501150077	24.06885758998435	21.282834966547984
37	24.60724075999622	31.067628740376634	22.79715579082249	21.527974708804653
38	24.28059783008266	31.165096469945702	23.14774553360431	21.40656016636733
39	23.100482087153797	29.20460871117833	25.395805106552814	22.29910409511506
40	23.928747728728826	29.756645765720346	24.997951917320478	21.316654588230353
41	22.826774217264813	29.89990652340591	25.661530705485713	21.61178855384357
42	22.240077301992418	29.96691558748464	24.199724821711776	23.593282288811164
43	23.63865519740366	28.388946655323437	25.342659986766236	22.629738160506662
44	23.51766077449034	29.822394471227064	23.857537469410047	22.802407284872547
45	22.95953198685026	29.553517975864136	24.431420739200302	23.055529298085307
46	24.191112371469682	26.891220552247113	24.52594763210133	24.39171944418187
47	23.348352606316496	27.319742466731782	25.226917057902977	24.104987869048745
48	23.427965256115364	27.135520055455775	25.501045047315962	23.935469641112896
49	23.288485574145845	28.28895820861035	25.93019714108664	22.492359076157168
50	24.11276008024283	29.028368570858408	24.330802113201205	22.528069235697558
51	22.18462152482381	28.54397075968113	25.80374116436126	23.4676665511338
52	23.272100912709664	28.41121299009568	25.187635882408543	23.129050214786105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	12.0
6	24.0
7	27.0
8	30.0
9	95.5
10	161.0
11	1172.0
12	2183.0
13	2505.5
14	3073.0
15	3318.0
16	2559.5
17	1801.0
18	1740.5
19	1680.0
20	1810.5
21	1941.0
22	1853.0
23	1765.0
24	2072.0
25	2379.0
26	2969.0
27	3559.0
28	3947.0
29	4335.0
30	5517.0
31	6699.0
32	7832.5
33	8966.0
34	9306.5
35	9647.0
36	11396.0
37	13145.0
38	14478.0
39	16531.5
40	17252.0
41	17781.0
42	18310.0
43	20601.5
44	22893.0
45	23645.0
46	24397.0
47	24394.5
48	24392.0
49	24871.5
50	25351.0
51	27034.5
52	28718.0
53	29711.0
54	30704.0
55	31315.0
56	31926.0
57	31895.5
58	31865.0
59	31323.5
60	30782.0
61	30821.5
62	30861.0
63	26929.5
64	21327.0
65	19656.0
66	16131.0
67	12606.0
68	11328.0
69	10050.0
70	7511.5
71	4973.0
72	4224.5
73	3476.0
74	3033.5
75	2591.0
76	1964.5
77	1338.0
78	824.0
79	310.0
80	280.5
81	251.0
82	161.5
83	72.0
84	41.0
85	10.0
86	5.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	476055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.775278066609951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0409725828573	12.153427650166472
2	9.254450791621727	2.919830691831826
3	3.2343972622804564	1.5307054857106845
4	1.561938241521192	0.9856004033147431
5	0.8428873886469859	0.6648391467372468
6	0.5632565014181281	0.5331316759618111
7	0.33688864032810023	0.37201583850605496
8	0.23968361762473533	0.3024860572832971
9	0.1970731967136713	0.2797996029870498
>10	3.2184183544388074	14.126939114177983
>50	2.1078842594442	23.834640955351798
>100	1.3701913474214038	37.695854470596885
>500	0.022636786109002785	2.4434151516106333
>1k	0.009321029574295264	2.1573137557635147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2000	0.42011952400457925	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG	1864	0.3915513963722679	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1451	0.3047967146653223	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	1448	0.30416653537931543	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1314	0.2760185272710086	No Hit
GAGATCGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAAAT	1135	0.23841782987259874	No Hit
GAGATCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	1058	0.22224322819842246	No Hit
GAGATCGGGGTGGTTATCCAGTCTGTGTGCCAAAGTCAGCCATATCTCCCAC	954	0.2003970129501843	No Hit
GAGATCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	946	0.19871653485416604	No Hit
GAGATCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	876	0.18401235151400572	No Hit
GAGATCGGGGCTGGAAGCAGAGACTCCTGTGCGTCTCTCTCACGCTACCCCT	846	0.17771055865393703	No Hit
GAGATCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	792	0.1663673315058134	No Hit
GAGATCGGAGATGGAGAAGACACCATACTGAAGCTAAAAGTGATACCTGAAA	776	0.16300637531377676	No Hit
GAGATCGGGGACTTCAAGTAGCCAAGCCGGCTTCGAGAGTCGTATCTATTGT	765	0.16069571793175158	No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	658	0.1382193233975066	No Hit
GAGATCGGGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTG	625	0.13128735125143104	No Hit
GAGATCGGGAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAGAC	604	0.12687609624938295	No Hit
GAGATCGGGGAGGGGGAGGTGGGGTAAGTGCCTCCAGGAAGGCAGGCAGCCT	593	0.12456543886735776	No Hit
GAGATCGGGACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCGCTGCC	584	0.12267490100933715	No Hit
GAGATCGGGAGCGTTCTTGTAATTTCACGCGCTTTTGTGATGGAGTGCTGTT	540	0.11343227148123641	No Hit
GAGATCGGGGTCCATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAG	538	0.11301215195723183	No Hit
GAGATCGGGCAGTCGGGCGTCTTGGCGCACGCCTTTAATCCCAGCATTCGGG	530	0.11133167386121352	No Hit
GAGATCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	504	0.10587012004915398	No Hit
GAGATCGGGTCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTA	501	0.10523994076314712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.7280461291237357	0.0	0.0	0.0	0.0
3	2.7280461291237357	0.0	0.0	0.0	0.0
4	2.7280461291237357	0.0	0.0	0.0	0.0
5	2.7280461291237357	0.0	0.0	0.0	0.0
6	2.7280461291237357	0.0	0.0	0.0	0.0
7	2.7414899538918824	0.0	0.0	0.0	0.0
8	2.76606694604615	0.0	0.0	0.0	0.0
9	2.7839220258163446	0.0	0.0	0.0	0.0
10	2.815851109640693	0.0	0.0	0.0	0.0
11	2.8503009106090684	0.0	0.0	0.0	0.0
12	2.8685761099032674	0.0	0.0	0.0	0.0
13	2.8990347753935994	0.0	0.0	0.0	0.0
14	2.976336767810442	0.0	0.0	0.0	0.0
15	3.004694835680751	0.0	0.0	0.0	0.0
16	3.047547027129218	0.0	0.0	0.0	0.0
17	3.0725441388074906	0.0	0.0	0.0	0.0
18	3.107624119061873	0.0	0.0	0.0	0.0
19	3.1353520076461754	0.0	0.0	0.0	0.0
20	3.172742645282583	0.0	0.0	0.0	0.0
21	3.2225268088771255	0.0	0.0	0.0	0.0
22	3.273571331043682	0.0	0.0	0.0	0.0
23	3.3302874667843003	0.0	0.0	0.0	0.0
24	3.3741899570427787	0.0	0.0	0.0	0.0
25	3.4138912520612115	0.0	0.0	0.0	0.0
26	3.4538026068416463	0.0	0.0	0.0	0.0
27	3.491613364002059	0.0	0.0	0.0	0.0
28	3.5552614718887523	0.0	0.0	0.0	0.0
29	3.5962231254791988	0.0	0.0	0.0	0.0
30	3.629202508113558	0.0	0.0	0.0	0.0
31	3.660921532175904	0.0	0.0	0.0	0.0
32	3.689069540284211	0.0	0.0	0.0	0.0
33	3.7151169507724946	0.0	0.0	0.0	0.0
34	3.753137767694909	0.0	0.0	0.0	0.0
35	3.790738465093319	0.0	0.0	0.0	0.0
36	3.8201468317736396	0.0	0.0	0.0	0.0
37	3.847454600833937	0.0	0.0	0.0	0.0
38	3.869930995368182	0.0	0.0	0.0	0.0
39	3.8963985253804707	0.0	0.0	0.0	0.0
40	3.920555398010734	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	40	5.5988494E-9	46.0	25
CAGGTCG	20	6.307712E-4	46.0	20
AACGTCT	25	3.414119E-5	46.0	17
CGGGTCG	25	3.414119E-5	46.0	6
CCTTATT	20	6.307712E-4	46.0	45
TCGTTGC	25	3.414119E-5	46.0	37
TCGTTAG	55	1.8189894E-12	46.0	28
ACCGGTA	25	3.414119E-5	46.0	24
CGAACCC	20	6.307712E-4	46.0	45
ACGCCTA	20	6.307712E-4	46.0	32
CGTATTT	20	6.307712E-4	46.0	13
CGTATTG	30	1.859089E-6	46.0	13
AGTCATA	25	3.414119E-5	46.0	42
CTCGTCA	25	3.414119E-5	46.0	28
GTACACC	20	6.307712E-4	46.0	21
GATCGAC	20	6.307712E-4	46.0	28
ATAGGCG	25	3.414119E-5	46.0	32
GTTTCGC	20	6.307712E-4	46.0	17
CGAAAGG	30	1.859089E-6	46.0	23
ACGTTGA	25	3.414119E-5	46.0	41
>>END_MODULE