FastQCFastQC Report
Fri 17 Jun 2016
SRR1527630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129621
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC73900.6542017189836237No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG41080.3636617945310861No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT36650.32444510149864425No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG24570.21750657964042805No Hit
ATCAACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA24530.2171524785746724No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23040.2039622138752732No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC22410.1983851220896212No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT21660.19174572710670215No Hit
ATCAACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20280.17952924033813109No Hit
ATCAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC18740.16589634930653732No Hit
ATCAACGGGGGGGTTGGATAGATGGCTCAGCAGTTAAGAGCACCGACTGC17400.15403396360372196No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC16310.14438470956187963No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC12250.10844345138767782No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT11680.10339751120065933No Hit
ATCAACGGGACGTGGCTGACGATGACTGGTCTTCCTGGCCATATTACTTT11410.10100732900680848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG254.4427914E-544.037
GATCGAC551.8189894E-1244.09
TAGCGGA207.856425E-444.038
ACCGATT302.5276786E-644.016
CGATTGG207.856425E-444.032
AGTATCG302.5276786E-644.028
ATATCGG351.4460602E-744.012
TACGCGT207.856425E-444.035
TACGATA207.856425E-444.037
CGCTAGT207.856425E-444.037
GTCGTGT302.5276786E-644.042
GATATCG207.856425E-444.011
CGAATAT351.4460602E-744.030
CTCGCGG207.856425E-444.043
GGTTATA207.856425E-444.08
TATCGGG207.856425E-444.012
CGATACC207.856425E-444.044
TCGGTCG207.856425E-444.014
TACGTTA207.856425E-444.044
TCTATCG207.856425E-444.037
Produced by FastQC (version 0.11.5)