##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129621
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.89286849306095	31.0	31.0	34.0	3.0	34.0
2	30.398801013791353	34.0	31.0	34.0	16.0	34.0
3	32.27626876624992	34.0	31.0	34.0	28.0	34.0
4	36.28627300661018	37.0	35.0	37.0	35.0	37.0
5	36.42134131713203	37.0	37.0	37.0	35.0	37.0
6	36.41965136979571	37.0	37.0	37.0	35.0	37.0
7	36.417785257179176	37.0	37.0	37.0	35.0	37.0
8	36.598576867816725	37.0	37.0	37.0	35.0	37.0
9	38.71920228111907	39.0	39.0	39.0	39.0	39.0
10	38.18650768709151	39.0	39.0	39.0	37.0	39.0
11	37.7928464502696	39.0	38.0	39.0	35.0	39.0
12	37.26350342282943	39.0	37.0	39.0	35.0	39.0
13	37.127958846374135	39.0	37.0	39.0	34.0	39.0
14	37.85719015492807	40.0	38.0	40.0	33.0	40.0
15	37.843841430001746	40.0	37.0	40.0	33.0	40.0
16	37.88219854269706	40.0	37.0	40.0	33.0	40.0
17	37.78697279884138	40.0	37.0	40.0	33.0	40.0
18	37.67899410510251	40.0	37.0	40.0	33.0	40.0
19	37.6146291543801	40.0	37.0	40.0	33.0	40.0
20	37.495878706220935	39.0	37.0	40.0	33.0	40.0
21	37.44955874580943	39.0	36.0	40.0	33.0	40.0
22	37.466182905594	39.0	36.0	40.0	33.0	40.0
23	37.428113500014604	39.0	36.0	40.0	33.0	40.0
24	37.36457803103873	39.0	36.0	40.0	33.0	40.0
25	37.337204248150485	39.0	36.0	40.0	33.0	40.0
26	37.21721444626118	39.0	36.0	40.0	33.0	40.0
27	37.0781669250129	39.0	35.0	40.0	33.0	40.0
28	36.949431712052096	39.0	35.0	40.0	32.0	40.0
29	37.147408732663436	39.0	36.0	40.0	33.0	40.0
30	37.41315184473376	40.0	36.0	40.0	33.0	40.0
31	37.50841653970668	40.0	36.0	40.0	33.0	40.0
32	37.49021840068483	40.0	36.0	40.0	33.0	40.0
33	37.396943753701464	40.0	36.0	40.0	33.0	40.0
34	37.01208635462691	40.0	35.0	40.0	32.0	40.0
35	37.008255866348094	40.0	35.0	40.0	32.0	40.0
36	37.087497488095565	40.0	35.0	40.0	33.0	40.0
37	37.06039459252263	39.0	35.0	40.0	33.0	40.0
38	36.995843738740696	39.0	35.0	40.0	33.0	40.0
39	36.91912862809739	39.0	35.0	40.0	33.0	40.0
40	36.78728175202125	39.0	35.0	40.0	32.0	40.0
41	36.65362099323578	39.0	35.0	40.0	32.0	40.0
42	36.59328659789434	39.0	35.0	40.0	32.0	40.0
43	36.46812072367635	39.0	35.0	40.0	32.0	40.0
44	36.12619630831934	38.0	35.0	40.0	31.0	40.0
45	36.097219332855886	38.0	35.0	40.0	31.0	40.0
46	35.94686093831471	38.0	35.0	40.0	31.0	40.0
47	35.88172935878494	38.0	35.0	40.0	31.0	40.0
48	35.80474778708965	37.0	35.0	40.0	31.0	40.0
49	35.7223909612162	37.0	35.0	40.0	30.0	40.0
50	35.191210149244746	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	15.0
15	27.0
16	54.0
17	107.0
18	143.0
19	239.0
20	414.0
21	706.0
22	1126.0
23	1703.0
24	2479.0
25	3601.0
26	4789.0
27	6870.0
28	9371.0
29	12328.0
30	16088.0
31	21831.0
32	31174.0
33	47973.0
34	85343.0
35	84068.0
36	115259.0
37	218682.0
38	352948.0
39	112279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.91344087972868	0.9146430528469283	0.11278118944318491	0.059134877981199006
8	99.23673515276363	0.4983972500511234	0.0985286215465187	0.16633897563873193
9	97.52129253971023	1.1834942870219305	0.5360204882876646	0.7591926849801837
10	52.4683057414832	30.0648624627198	8.166544354256871	9.300287441540126
11	35.80599156708312	24.00681290450514	22.48382422068995	17.703371307721795
12	32.54994374219318	17.706292641514278	24.301867617546062	25.44189599874648
13	27.347667934643567	18.614384824644723	26.758974912824744	27.27897232788696
14	26.402218089075895	19.764593611485623	27.698847666606763	26.13434063283172
15	27.73903813757003	20.945343615247946	26.53544861506647	24.780169632115552
16	32.401929496707304	20.937376341268443	24.418101292380364	22.24259286964389
17	30.80457958908342	22.1709759290948	22.894581456966538	24.12986302485524
18	29.624537787452603	21.494023216636375	23.877300439705	25.00413855620602
19	27.742225047161835	23.326938858254227	23.369165410345595	25.56167068423834
20	28.67112066790543	23.463710394902364	23.951130511915057	23.914038425277152
21	29.019202015543264	22.430532010293717	26.267925259888052	22.282340714274966
22	27.789320488907343	21.563515550790928	26.644600268585663	24.002563691716073
23	27.99514173337783	22.374318466105002	26.568911165780378	23.061628634736785
24	28.459545281116412	22.744973756684765	24.49901338590554	24.29646757629329
25	27.814815765641747	23.192557503799947	23.850388758707567	25.142237971850733
26	25.690209371107652	22.592179146811187	24.92951175659801	26.78809972548315
27	25.176674300495478	21.744549720658522	24.553456424765475	28.525319554080532
28	25.73323265059697	23.90987773775452	24.354451625810782	26.00243798583773
29	27.559508897231904	23.223983973385764	23.335437283832363	25.88106984554997
30	27.527462750781012	21.26677885768767	24.42252755570231	26.78323083582901
31	28.693783136113794	22.59518900587011	23.622790298693104	25.088237559322994
32	30.17144688351226	22.60696286630649	24.218830917626356	23.002759332554902
33	30.595748485554005	21.52164309976532	25.36912822973369	22.51348018494699
34	26.27934501925867	23.498500824612854	27.474524641450536	22.747629514677932
35	25.755983644071772	25.488283238360477	27.1855781717939	21.570154945773847
36	25.55759852198215	26.844224744405427	24.175984688669917	23.42219204494251
37	27.324562840103006	26.777211117711165	22.65653701551228	23.241689026673548
38	27.204876679877586	26.519602592373904	23.416437902623976	22.85908282512453
39	27.98965316685862	24.727320048051517	22.907948772198814	24.37507801289105
40	27.17247643236094	24.780700783714185	22.51409986181206	25.532722922112818
41	24.526279167968728	25.348767418452738	24.299211859552894	25.825741554025644
42	23.548429074884407	23.79576866931475	24.664201533080565	27.99160072272028
43	23.00284785782134	23.7870046679373	25.484742227702924	27.725405246538443
44	24.94668565828716	23.51487799890406	25.145070780376784	26.393365562432002
45	26.123097923993978	23.87136924685359	23.526209233008238	26.479323596144194
46	25.097886813364838	25.87699768329378	22.68769790929878	26.337417594042606
47	24.648178459855117	23.851628112437712	25.906830698083695	25.593362729623475
48	26.245970993811195	23.253020260777728	25.03361746993018	25.467391275480892
49	26.498976205293634	23.243459532002326	24.232729384457265	26.02483487824678
50	24.481131282084874	23.051536754362747	26.061926964884684	26.40540499866769
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	14.5
4	29.0
5	19.5
6	10.0
7	25.5
8	41.0
9	409.0
10	777.0
11	954.0
12	1131.0
13	1640.5
14	2150.0
15	2043.0
16	1936.0
17	2022.0
18	2108.0
19	2216.0
20	2324.0
21	2154.5
22	1985.0
23	2874.5
24	3764.0
25	4402.5
26	5041.0
27	6450.5
28	7860.0
29	9310.0
30	10760.0
31	10783.0
32	10806.0
33	13859.5
34	16913.0
35	17674.0
36	18435.0
37	22301.0
38	26167.0
39	27313.0
40	28459.0
41	32281.5
42	36104.0
43	40997.5
44	45891.0
45	50725.5
46	55560.0
47	60041.5
48	64523.0
49	68273.5
50	72024.0
51	77639.0
52	83254.0
53	85667.5
54	88081.0
55	93737.0
56	99393.0
57	103972.5
58	108552.0
59	100875.0
60	93198.0
61	89302.5
62	85407.0
63	72854.0
64	60301.0
65	48240.0
66	36179.0
67	27828.5
68	19478.0
69	17430.5
70	15383.0
71	12793.5
72	10204.0
73	9414.5
74	8625.0
75	6276.5
76	3928.0
77	2697.0
78	1466.0
79	1003.5
80	541.0
81	608.5
82	676.0
83	400.0
84	124.0
85	71.0
86	18.0
87	13.0
88	8.0
89	6.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1129621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.888014653493206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.24482724332147	5.976730099102604
2	9.350563492236212	1.6621589067482814
3	3.6118366991908553	0.9630617252529857
4	1.7588916143222535	0.6253221776801005
5	0.9729396367453329	0.43237508741784375
6	0.6179616536774291	0.329547133990913
7	0.4399726982895864	0.27373386526743526
8	0.33097946166550507	0.23534002442306418
9	0.32098008215893614	0.25675881063372696
>10	8.400478723418098	22.225678990817414
>50	4.865698067867214	29.85192225203907
>100	2.0138750326108856	30.365084374780203
>500	0.05099683548319522	2.9954305827618875
>1k	0.018998821062366843	3.150072441710549
>5k	9.999379506508866E-4	0.6567835273739125
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7390	0.6542017189836237	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4108	0.3636617945310861	No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3665	0.32444510149864425	No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	2457	0.21750657964042805	No Hit
ATCAACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	2453	0.2171524785746724	No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2304	0.2039622138752732	No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC	2241	0.1983851220896212	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2166	0.19174572710670215	No Hit
ATCAACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2028	0.17952924033813109	No Hit
ATCAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1874	0.16589634930653732	No Hit
ATCAACGGGGGGGTTGGATAGATGGCTCAGCAGTTAAGAGCACCGACTGC	1740	0.15403396360372196	No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1631	0.14438470956187963	No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1225	0.10844345138767782	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT	1168	0.10339751120065933	No Hit
ATCAACGGGACGTGGCTGACGATGACTGGTCTTCCTGGCCATATTACTTT	1141	0.10100732900680848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.03806586456873588	0.0	0.0	0.0	0.0
8	0.043200330022193284	0.0	0.0	0.0	0.0
9	0.04674134067975011	0.0	0.0	0.0	0.0
10	0.056656170520909224	0.0	0.0	0.0	0.0
11	0.06373819183602288	0.0	0.0	0.0	0.0
12	0.0715284152826479	0.0	0.0	0.0	0.0
13	0.08188587145600161	0.0	0.0	0.0	0.0
14	0.08932199383687095	0.0	0.0	0.0	0.0
15	0.09905977314515223	0.0	0.0	0.0	0.0
16	0.1187123822945926	0.0	0.0	0.0	0.0
17	0.12968951533301878	0.0	0.0	0.0	0.0
18	0.14066664837144494	0.0	0.0	0.0	0.0
19	0.1512896803441154	0.0	0.0	0.0	0.0
20	0.16031925752088533	0.0	0.0	8.852526643892066E-5	0.0
21	0.17023408736204443	0.0	0.0	8.852526643892066E-5	0.0
22	0.17961776560457002	0.0	0.0	8.852526643892066E-5	0.0
23	0.19165720184026325	0.0	0.0	8.852526643892066E-5	0.0
24	0.20130645588210558	0.0	0.0	8.852526643892066E-5	0.0
25	0.2107786593910701	0.0	0.0	8.852526643892066E-5	0.0
26	0.22007381236715678	0.0	0.0	8.852526643892066E-5	0.0
27	0.22892633901104883	0.0	0.0	8.852526643892066E-5	0.0
28	0.24016904784879176	0.0	0.0	8.852526643892066E-5	0.0
29	0.24981830189063411	0.0	0.0	8.852526643892066E-5	0.0
30	0.26114953599481594	0.0	0.0	8.852526643892066E-5	0.0
31	0.27177256796748644	0.0	0.0	8.852526643892066E-5	0.0
32	0.28168739780864555	0.0	0.0	8.852526643892066E-5	0.0
33	0.29107107605117116	0.0	0.0	8.852526643892066E-5	0.0
34	0.30134000695808594	0.0	0.0	8.852526643892066E-5	0.0
35	0.31293681686158453	0.0	0.0	8.852526643892066E-5	0.0
36	0.3264811826267394	0.0	0.0	8.852526643892066E-5	0.0
37	0.3382550430631159	0.0	0.0	8.852526643892066E-5	0.0
38	0.3499403782330534	0.0	0.0	8.852526643892066E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	25	4.4427914E-5	44.0	37
GATCGAC	55	1.8189894E-12	44.0	9
TAGCGGA	20	7.856425E-4	44.0	38
ACCGATT	30	2.5276786E-6	44.0	16
CGATTGG	20	7.856425E-4	44.0	32
AGTATCG	30	2.5276786E-6	44.0	28
ATATCGG	35	1.4460602E-7	44.0	12
TACGCGT	20	7.856425E-4	44.0	35
TACGATA	20	7.856425E-4	44.0	37
CGCTAGT	20	7.856425E-4	44.0	37
GTCGTGT	30	2.5276786E-6	44.0	42
GATATCG	20	7.856425E-4	44.0	11
CGAATAT	35	1.4460602E-7	44.0	30
CTCGCGG	20	7.856425E-4	44.0	43
GGTTATA	20	7.856425E-4	44.0	8
TATCGGG	20	7.856425E-4	44.0	12
CGATACC	20	7.856425E-4	44.0	44
TCGGTCG	20	7.856425E-4	44.0	14
TACGTTA	20	7.856425E-4	44.0	44
TCTATCG	20	7.856425E-4	44.0	37
>>END_MODULE