FastQCFastQC Report
Fri 17 Jun 2016
SRR1527629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445011
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC96010.664424007844923No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT54760.37895905290686366No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49430.3420735205475945No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC32050.22179761953369215No Hit
ATCAACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG31490.2179222165090785No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT30970.21432362798622295No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC28070.19425457660875936No Hit
ATCAACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC26700.18477367992354382No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT24000.16608870105487086No Hit
ATCAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT23720.16415099954256404No Hit
ATCAACGGGGGGGTTGGATAGATGGCTCAGCAGTTAAGAGCACCGACTGCTC21680.15003345995290002No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA21540.1490646091967466No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC15940.11031057895061007No Hit
ATCAACGGGACGTGGCTGACGATGACTGGTCTTCCTGGCCATATTACTTTCT15640.10823447018742417No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT15510.1073348230567103No Hit
ATCAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC14570.10082968226539453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG351.0198164E-746.00000437
TTTACGA405.6134013E-946.00000433
CGAAACG206.311995E-446.00000425
CGAAACA405.6134013E-946.00000424
GACCGTA206.311995E-446.00000423
ATTACGC206.311995E-446.00000436
TAACGGA206.311995E-446.00000412
TTATCGC206.311995E-446.00000437
AATGCGG206.311995E-446.00000419
TTAGTCG206.311995E-446.00000416
ATGCGCG206.311995E-446.00000437
TTACGCC206.311995E-446.00000437
ATACGCG206.311995E-446.00000440
CAATCTA206.311995E-446.00000414
GCGTATT206.311995E-446.00000413
TCGCGTA206.311995E-446.00000411
TCGGTAC206.311995E-446.00000414
CGCCCAT351.0198164E-746.00000411
CGCTTAT206.311995E-446.00000428
ACGCGGT351.0198164E-746.00000426
Produced by FastQC (version 0.11.5)