##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1445011
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11494306963753	33.0	31.0	34.0	31.0	34.0
2	32.645149414087506	34.0	31.0	34.0	31.0	34.0
3	32.69822098240083	34.0	31.0	34.0	31.0	34.0
4	36.43246314387918	37.0	37.0	37.0	35.0	37.0
5	36.43921464957706	37.0	37.0	37.0	35.0	37.0
6	36.276350837467675	37.0	37.0	37.0	35.0	37.0
7	36.32924316839111	37.0	35.0	37.0	35.0	37.0
8	36.54018343112959	37.0	37.0	37.0	35.0	37.0
9	38.66209253770386	39.0	39.0	39.0	38.0	39.0
10	37.99075231953252	39.0	38.0	39.0	35.0	39.0
11	37.58254850655116	39.0	37.0	39.0	35.0	39.0
12	37.0975715755797	39.0	37.0	39.0	34.0	39.0
13	36.98084305240583	39.0	37.0	39.0	33.0	39.0
14	37.73363040142947	40.0	37.0	40.0	33.0	40.0
15	37.753780421048695	40.0	37.0	40.0	33.0	40.0
16	37.79806935725749	40.0	37.0	40.0	33.0	40.0
17	37.72951486182458	40.0	37.0	40.0	33.0	40.0
18	37.7048375410291	40.0	37.0	40.0	33.0	40.0
19	37.67104817887199	40.0	37.0	40.0	33.0	40.0
20	37.62810179299673	40.0	37.0	40.0	33.0	40.0
21	37.59899682424563	40.0	37.0	40.0	33.0	40.0
22	37.572428860403136	40.0	36.0	40.0	33.0	40.0
23	37.56815000024221	40.0	36.0	40.0	33.0	40.0
24	37.508418275016595	40.0	36.0	40.0	33.0	40.0
25	37.50272627682419	40.0	36.0	40.0	33.0	40.0
26	37.37590440487996	40.0	36.0	40.0	33.0	40.0
27	37.28806631921833	39.0	36.0	40.0	33.0	40.0
28	37.20445449896229	39.0	35.0	40.0	33.0	40.0
29	37.10783516526864	39.0	35.0	40.0	33.0	40.0
30	37.02471815093449	39.0	35.0	40.0	32.0	40.0
31	36.92383656595002	39.0	35.0	40.0	32.0	40.0
32	36.80826305128473	39.0	35.0	40.0	32.0	40.0
33	36.703199491214946	39.0	35.0	40.0	31.0	40.0
34	36.447806279675376	39.0	35.0	40.0	31.0	40.0
35	36.342188398565824	38.0	35.0	40.0	30.0	40.0
36	36.397620502542885	38.0	35.0	40.0	31.0	40.0
37	36.354907332885354	38.0	35.0	40.0	31.0	40.0
38	36.27033081409069	38.0	35.0	40.0	31.0	40.0
39	36.20078393866898	38.0	35.0	40.0	31.0	40.0
40	36.03750974906073	38.0	35.0	40.0	30.0	40.0
41	35.894884537211134	38.0	35.0	40.0	30.0	40.0
42	35.8435825056003	38.0	34.0	40.0	30.0	40.0
43	35.68872762906303	38.0	34.0	40.0	30.0	40.0
44	35.5755084217352	37.0	34.0	40.0	30.0	40.0
45	35.442392480057244	37.0	34.0	40.0	29.0	40.0
46	35.328267397272406	37.0	34.0	40.0	29.0	40.0
47	35.120156178741894	37.0	34.0	40.0	29.0	40.0
48	34.91369754278687	36.0	34.0	40.0	28.0	40.0
49	35.30865370575034	37.0	34.0	40.0	29.0	40.0
50	35.39959280586791	37.0	34.0	40.0	30.0	40.0
51	35.419067398102854	36.0	34.0	40.0	30.0	40.0
52	34.97252962088178	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	4.0
15	14.0
16	41.0
17	88.0
18	182.0
19	370.0
20	618.0
21	1089.0
22	1774.0
23	2804.0
24	4244.0
25	5940.0
26	8162.0
27	11006.0
28	14751.0
29	18850.0
30	24609.0
31	31533.0
32	41994.0
33	60299.0
34	114847.0
35	116759.0
36	148530.0
37	234892.0
38	468399.0
39	133211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.06062998828382	0.8335576684191331	0.0759163771071639	0.029895966189876756
8	99.33924378430338	0.45051560161133725	0.05674697286041421	0.1534936412248765
9	97.57441292834449	1.1360467152153169	0.4996501756734032	0.78989018076679
10	52.61440916366726	29.88212546478885	8.203605370478147	9.299860001065737
11	35.91017646232451	24.030405304873113	22.356092790989134	17.703325441813245
12	32.65885173192453	17.617443742642788	24.35995296921615	25.363751556216528
13	27.577575534027076	18.612245858336028	26.65654448305238	27.15363412458452
14	26.5800744769417	19.686770550535602	27.574668981758617	26.158485990764085
15	27.777435604296436	20.900671344370387	26.541943279324514	24.779949772008656
16	32.42847286283634	20.804547508634883	24.43552332819612	22.33145630033266
17	30.898519111619223	22.061977382871135	22.82605461134898	24.213448894160667
18	29.701504002391676	21.4722932905009	23.891582832241415	24.934619874866005
19	27.85563570104311	23.274701715073448	23.466464961166388	25.403197622717062
20	28.609055571203264	23.376915469847635	24.006184035969277	24.007844922979828
21	29.091335636891348	22.417822424881194	26.299038554031767	22.19180338419569
22	27.873628643657383	21.52807141260516	26.63142356701783	23.966876376719622
23	27.917503742186046	22.48744127207336	26.46671893847175	23.128336047268842
24	28.282345255503245	22.739965301302203	24.59081626368242	24.38687317951213
25	27.752314688261887	23.14764385876647	23.900856118050314	25.199185334921324
26	25.640496854349205	22.437199440004264	24.944515993303856	26.97778771234268
27	25.122922939686966	21.819972304709097	24.491924282929332	28.5651804726746
28	25.707970389152745	23.900510099923114	24.24528256186285	26.146236949061286
29	27.579374828288504	23.20127666848211	23.27878472897438	25.940563774255004
30	27.571554818613837	21.22537475493266	24.343620913612423	26.859449512841078
31	28.77424462512742	22.572492527738543	23.52162025064169	25.13164259649234
32	30.175618040277897	22.403220459913452	24.24216839871807	23.17899310109058
33	30.509317922147304	21.511600949750555	25.40444328797497	22.574637840127167
34	26.22028482828158	23.365289260773796	27.527748923710615	22.886676987234004
35	25.790253499800347	25.53537654730656	27.009621380044855	21.664748572848232
36	25.46222831521698	26.842702235484712	24.30957272989617	23.385496719402134
37	27.350310828083664	26.8087232553939	22.689792672858548	23.151173243663887
38	27.250519200199857	26.444227760203905	23.479752057250774	22.825500982345464
39	28.020271125963745	24.775520739980525	22.87484316728385	24.32936496677188
40	27.24477529928838	24.768185155683938	22.470555587466116	25.51648395756157
41	24.521128212864816	25.31966884681155	24.275801360681683	25.88340157964196
42	23.61746727187544	23.786877746951408	24.656905725977172	27.938749255195983
43	23.14342243761466	23.60591026642704	25.5209129895897	27.729754306368605
44	24.712407033579677	23.494630836720273	25.194341081140557	26.59862104855949
45	26.165613964184352	23.781133846039925	23.465426906784792	26.587825282990924
46	24.881540694153887	25.689562224785835	22.888337874244556	26.54055920681573
47	24.638359154359378	23.71725889975924	25.94776095130072	25.696620994580666
48	26.201184627660272	23.157609180829766	25.08825192334176	25.5529542681682
49	26.483673826704436	23.02639910699642	24.373032454424223	26.116894611874926
50	24.460367429728908	22.941278647705797	26.2234681950518	26.37488572751349
51	24.442997319743586	23.239477069724728	25.839111259360653	26.478414351171033
52	24.052342854137443	24.18929682888227	26.06367702391193	25.69468329306836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	15.0
4	30.0
5	21.5
6	13.0
7	33.0
8	53.0
9	506.5
10	960.0
11	1130.0
12	1300.0
13	1947.5
14	2378.0
15	2161.0
16	2291.5
17	2422.0
18	2604.0
19	2786.0
20	2545.0
21	2304.0
22	2796.0
23	3288.0
24	3938.0
25	4588.0
26	6503.0
27	8418.0
28	9939.0
29	11460.0
30	12252.5
31	13045.0
32	14727.5
33	16410.0
34	18361.0
35	20312.0
36	23225.5
37	26139.0
38	29334.5
39	34360.0
40	36190.0
41	41821.0
42	47452.0
43	53385.5
44	59319.0
45	65466.0
46	71613.0
47	73367.5
48	75122.0
49	83271.0
50	91420.0
51	95516.0
52	99612.0
53	104884.5
54	110157.0
55	119684.5
56	129212.0
57	129754.5
58	130297.0
59	122023.5
60	113750.0
61	108390.0
62	103030.0
63	92499.5
64	68591.5
65	55214.0
66	42627.0
67	30040.0
68	24812.5
69	19585.0
70	17848.5
71	16112.0
72	14605.0
73	13098.0
74	9659.0
75	6220.0
76	4568.0
77	2916.0
78	1915.5
79	915.0
80	764.0
81	613.0
82	434.0
83	255.0
84	151.5
85	48.0
86	33.5
87	19.0
88	14.5
89	8.0
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1445011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.664430445837785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.51999242278191	5.098378551820685
2	9.604566930821882	1.4722707040735603
3	4.065142865279463	0.9347101422998448
4	2.1843624360198195	0.6696757583749877
5	1.2151173372454007	0.46565911574244945
6	0.7714933490844402	0.35478342680904856
7	0.4887294484528993	0.2622083005149937
8	0.36406242082339063	0.22322648818753626
9	0.2728209314480882	0.18819153479272951
>10	6.0851713609251	13.759861230929754
>50	5.397698764231268	29.58082822750643
>100	2.92785635060645	38.45311030890716
>500	0.07769072698307766	4.076513811647805
>1k	0.02348789420418627	3.4166663929202494
>5k	0.0018067610926297132	1.0439160054727554
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9601	0.664424007844923	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5476	0.37895905290686366	No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4943	0.3420735205475945	No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	3205	0.22179761953369215	No Hit
ATCAACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	3149	0.2179222165090785	No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3097	0.21432362798622295	No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC	2807	0.19425457660875936	No Hit
ATCAACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2670	0.18477367992354382	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2400	0.16608870105487086	No Hit
ATCAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2372	0.16415099954256404	No Hit
ATCAACGGGGGGGTTGGATAGATGGCTCAGCAGTTAAGAGCACCGACTGCTC	2168	0.15003345995290002	No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2154	0.1490646091967466	No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	1594	0.11031057895061007	No Hit
ATCAACGGGACGTGGCTGACGATGACTGGTCTTCCTGGCCATATTACTTTCT	1564	0.10823447018742417	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT	1551	0.1073348230567103	No Hit
ATCAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1457	0.10082968226539453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.03356375833817182	0.0	0.0	0.0	0.0
8	0.03771597586454359	0.0	0.0	0.0	0.0
9	0.04207580426723395	0.0	0.0	0.0	0.0
10	0.052317940832284325	0.0	0.0	0.0	0.0
11	0.05875387799816057	0.0	0.0	0.0	0.0
12	0.06498220428771823	0.0	0.0	0.0	0.0
13	0.07460150822381283	0.0	0.0	0.0	0.0
14	0.08186788889496342	0.0	0.0	0.0	0.0
15	0.08920347319155356	0.0	0.0	0.0	0.0
16	0.10892650644181948	0.0	0.0	0.0	0.0
17	0.1181305886252769	0.0	0.0	0.0	0.0
18	0.12650422730345998	0.0	0.0	0.0	0.0
19	0.13750760374834517	0.0	0.0	0.0	0.0
20	0.1467116859318026	0.0	0.0	0.0	0.0
21	0.15695382249685297	0.0	0.0	0.0	0.0
22	0.16470462854608028	0.0	0.0	0.0	0.0
23	0.17446233973305394	0.0	0.0	0.0	0.0
24	0.18387403279282996	0.0	0.0	0.0	0.0
25	0.1924552823473316	0.0	0.0	0.0	0.0
26	0.2024898080360634	0.0	0.0	0.0	0.0
27	0.20934096695457682	0.0	0.0	0.0	0.0
28	0.22152080503193403	0.0	0.0	0.0	0.0
29	0.23100170171714957	0.0	0.0	0.0	0.0
30	0.2384064896391792	0.0	0.0	0.0	0.0
31	0.2470569428191204	0.0	0.0	0.0	0.0
32	0.25743748663504984	0.0	0.0	0.0	0.0
33	0.26553431081147477	0.0	0.0	0.0	0.0
34	0.2756380401256461	0.0	0.0	0.0	0.0
35	0.28774867457756376	0.0	0.0	0.0	0.0
36	0.2983368292698118	0.0	0.0	0.0	0.0
37	0.3102398528454109	0.0	0.0	0.0	0.0
38	0.31868269514903347	0.0	0.0	0.0	0.0
39	0.32802518458336993	0.0	0.0	0.0	0.0
40	0.3388901537773761	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	35	1.0198164E-7	46.000004	37
TTTACGA	40	5.6134013E-9	46.000004	33
CGAAACG	20	6.311995E-4	46.000004	25
CGAAACA	40	5.6134013E-9	46.000004	24
GACCGTA	20	6.311995E-4	46.000004	23
ATTACGC	20	6.311995E-4	46.000004	36
TAACGGA	20	6.311995E-4	46.000004	12
TTATCGC	20	6.311995E-4	46.000004	37
AATGCGG	20	6.311995E-4	46.000004	19
TTAGTCG	20	6.311995E-4	46.000004	16
ATGCGCG	20	6.311995E-4	46.000004	37
TTACGCC	20	6.311995E-4	46.000004	37
ATACGCG	20	6.311995E-4	46.000004	40
CAATCTA	20	6.311995E-4	46.000004	14
GCGTATT	20	6.311995E-4	46.000004	13
TCGCGTA	20	6.311995E-4	46.000004	11
TCGGTAC	20	6.311995E-4	46.000004	14
CGCCCAT	35	1.0198164E-7	46.000004	11
CGCTTAT	20	6.311995E-4	46.000004	28
ACGCGGT	35	1.0198164E-7	46.000004	26
>>END_MODULE